Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats.
- Autores
- Pampillo, Macarena; del Carmen Díaz, María; Duvilanski, Beatriz Haydee; Rettori, Valeria; Seilicovich, Adriana; Lasaga, Mercedes Isabel
- Año de publicación
- 2001
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In order to determine whether ionotropic (iGluRs) and metabotropic (mGluRs) glutamate receptor activation modulates oxytocin release in male rats, we investigated the effect of agonists of both types of glutamate receptors on oxytocin release from hypothalamus and posterior pituitary. Kainate and quisqualate (1 mM) increased hypothalamic oxytocin release. Their effects were prevented by selective AMPA/kainate receptor antagonists. NMDA (0.01-1 mM) did not modify hypothalamic oxytocin release. Group I mGluR agonists, such as quisqualate and 3-HPG, significantly increased hypothalamic oxytocin release. These effects were blocked by AIDA (a selective antagonist of group I mGluRs). In the posterior pituitary, oxytocin release was not modified by kainate, quisqualate, trans-ACPD (a broad-spectrum mGluR agonist) and L-SOP (a group III mGluR agonist). However, NMDA (0.1 mM) significantly decreased oxytocin release from posterior pituitary. D-Aspartate significantly increased oxytocin release from the hypothalamus, while it decreased oxytocin release from posterior pituitary. AP-5 (a specific NMDA receptor antagonist) reduced the D-Aspartate effect in the hypothalamus, but not in the posterior pituitary. Our data indicate that the activation of non-NMDA receptors and group I mGluRs stimulates oxytocin release from hypothalamic nuclei, whereas NMDA inhibits oxytocinergic terminals in the posterior pituitary. D-Aspartate also has a dual effect on oxytocin release: stimulatory at the hypothalamus and inhibitory at the posterior pituitary. These results suggest that excitatory amino acids differentially modulate the secretion of oxytocin at the hypothalamic and posterior pituitary levels.
Fil: Pampillo, Macarena. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: del Carmen Díaz, María. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rettori, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina - Materia
-
GLUTAMATE AGONISTS
HYPOTHALAMUS
OXYTOCIN RELEASE
POSTERIOR PITUITARY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/123581
Ver los metadatos del registro completo
id |
CONICETDig_58ef21579e66954905137499ab770493 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/123581 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats.Pampillo, Macarenadel Carmen Díaz, MaríaDuvilanski, Beatriz HaydeeRettori, ValeriaSeilicovich, AdrianaLasaga, Mercedes IsabelGLUTAMATE AGONISTSHYPOTHALAMUSOXYTOCIN RELEASEPOSTERIOR PITUITARYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In order to determine whether ionotropic (iGluRs) and metabotropic (mGluRs) glutamate receptor activation modulates oxytocin release in male rats, we investigated the effect of agonists of both types of glutamate receptors on oxytocin release from hypothalamus and posterior pituitary. Kainate and quisqualate (1 mM) increased hypothalamic oxytocin release. Their effects were prevented by selective AMPA/kainate receptor antagonists. NMDA (0.01-1 mM) did not modify hypothalamic oxytocin release. Group I mGluR agonists, such as quisqualate and 3-HPG, significantly increased hypothalamic oxytocin release. These effects were blocked by AIDA (a selective antagonist of group I mGluRs). In the posterior pituitary, oxytocin release was not modified by kainate, quisqualate, trans-ACPD (a broad-spectrum mGluR agonist) and L-SOP (a group III mGluR agonist). However, NMDA (0.1 mM) significantly decreased oxytocin release from posterior pituitary. D-Aspartate significantly increased oxytocin release from the hypothalamus, while it decreased oxytocin release from posterior pituitary. AP-5 (a specific NMDA receptor antagonist) reduced the D-Aspartate effect in the hypothalamus, but not in the posterior pituitary. Our data indicate that the activation of non-NMDA receptors and group I mGluRs stimulates oxytocin release from hypothalamic nuclei, whereas NMDA inhibits oxytocinergic terminals in the posterior pituitary. D-Aspartate also has a dual effect on oxytocin release: stimulatory at the hypothalamus and inhibitory at the posterior pituitary. These results suggest that excitatory amino acids differentially modulate the secretion of oxytocin at the hypothalamic and posterior pituitary levels.Fil: Pampillo, Macarena. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: del Carmen Díaz, María. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rettori, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaHumana Press2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123581Pampillo, Macarena; del Carmen Díaz, María; Duvilanski, Beatriz Haydee; Rettori, Valeria; Seilicovich, Adriana; et al.; Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats.; Humana Press; Endocrine; 15; 3; 12-2001; 309-3150969-711X1355-008XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1385%2FENDO%3A15%3A3%3A309info:eu-repo/semantics/altIdentifier/doi/10.1385/ENDO:15:3:309info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:47Zoai:ri.conicet.gov.ar:11336/123581instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:47.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
title |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
spellingShingle |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. Pampillo, Macarena GLUTAMATE AGONISTS HYPOTHALAMUS OXYTOCIN RELEASE POSTERIOR PITUITARY |
title_short |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
title_full |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
title_fullStr |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
title_full_unstemmed |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
title_sort |
Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. |
dc.creator.none.fl_str_mv |
Pampillo, Macarena del Carmen Díaz, María Duvilanski, Beatriz Haydee Rettori, Valeria Seilicovich, Adriana Lasaga, Mercedes Isabel |
author |
Pampillo, Macarena |
author_facet |
Pampillo, Macarena del Carmen Díaz, María Duvilanski, Beatriz Haydee Rettori, Valeria Seilicovich, Adriana Lasaga, Mercedes Isabel |
author_role |
author |
author2 |
del Carmen Díaz, María Duvilanski, Beatriz Haydee Rettori, Valeria Seilicovich, Adriana Lasaga, Mercedes Isabel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
GLUTAMATE AGONISTS HYPOTHALAMUS OXYTOCIN RELEASE POSTERIOR PITUITARY |
topic |
GLUTAMATE AGONISTS HYPOTHALAMUS OXYTOCIN RELEASE POSTERIOR PITUITARY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In order to determine whether ionotropic (iGluRs) and metabotropic (mGluRs) glutamate receptor activation modulates oxytocin release in male rats, we investigated the effect of agonists of both types of glutamate receptors on oxytocin release from hypothalamus and posterior pituitary. Kainate and quisqualate (1 mM) increased hypothalamic oxytocin release. Their effects were prevented by selective AMPA/kainate receptor antagonists. NMDA (0.01-1 mM) did not modify hypothalamic oxytocin release. Group I mGluR agonists, such as quisqualate and 3-HPG, significantly increased hypothalamic oxytocin release. These effects were blocked by AIDA (a selective antagonist of group I mGluRs). In the posterior pituitary, oxytocin release was not modified by kainate, quisqualate, trans-ACPD (a broad-spectrum mGluR agonist) and L-SOP (a group III mGluR agonist). However, NMDA (0.1 mM) significantly decreased oxytocin release from posterior pituitary. D-Aspartate significantly increased oxytocin release from the hypothalamus, while it decreased oxytocin release from posterior pituitary. AP-5 (a specific NMDA receptor antagonist) reduced the D-Aspartate effect in the hypothalamus, but not in the posterior pituitary. Our data indicate that the activation of non-NMDA receptors and group I mGluRs stimulates oxytocin release from hypothalamic nuclei, whereas NMDA inhibits oxytocinergic terminals in the posterior pituitary. D-Aspartate also has a dual effect on oxytocin release: stimulatory at the hypothalamus and inhibitory at the posterior pituitary. These results suggest that excitatory amino acids differentially modulate the secretion of oxytocin at the hypothalamic and posterior pituitary levels. Fil: Pampillo, Macarena. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: del Carmen Díaz, María. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Duvilanski, Beatriz Haydee. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rettori, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Seilicovich, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina |
description |
In order to determine whether ionotropic (iGluRs) and metabotropic (mGluRs) glutamate receptor activation modulates oxytocin release in male rats, we investigated the effect of agonists of both types of glutamate receptors on oxytocin release from hypothalamus and posterior pituitary. Kainate and quisqualate (1 mM) increased hypothalamic oxytocin release. Their effects were prevented by selective AMPA/kainate receptor antagonists. NMDA (0.01-1 mM) did not modify hypothalamic oxytocin release. Group I mGluR agonists, such as quisqualate and 3-HPG, significantly increased hypothalamic oxytocin release. These effects were blocked by AIDA (a selective antagonist of group I mGluRs). In the posterior pituitary, oxytocin release was not modified by kainate, quisqualate, trans-ACPD (a broad-spectrum mGluR agonist) and L-SOP (a group III mGluR agonist). However, NMDA (0.1 mM) significantly decreased oxytocin release from posterior pituitary. D-Aspartate significantly increased oxytocin release from the hypothalamus, while it decreased oxytocin release from posterior pituitary. AP-5 (a specific NMDA receptor antagonist) reduced the D-Aspartate effect in the hypothalamus, but not in the posterior pituitary. Our data indicate that the activation of non-NMDA receptors and group I mGluRs stimulates oxytocin release from hypothalamic nuclei, whereas NMDA inhibits oxytocinergic terminals in the posterior pituitary. D-Aspartate also has a dual effect on oxytocin release: stimulatory at the hypothalamus and inhibitory at the posterior pituitary. These results suggest that excitatory amino acids differentially modulate the secretion of oxytocin at the hypothalamic and posterior pituitary levels. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/123581 Pampillo, Macarena; del Carmen Díaz, María; Duvilanski, Beatriz Haydee; Rettori, Valeria; Seilicovich, Adriana; et al.; Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats.; Humana Press; Endocrine; 15; 3; 12-2001; 309-315 0969-711X 1355-008X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/123581 |
identifier_str_mv |
Pampillo, Macarena; del Carmen Díaz, María; Duvilanski, Beatriz Haydee; Rettori, Valeria; Seilicovich, Adriana; et al.; Differental effects of glatamate agonist and D-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats.; Humana Press; Endocrine; 15; 3; 12-2001; 309-315 0969-711X 1355-008X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1385%2FENDO%3A15%3A3%3A309 info:eu-repo/semantics/altIdentifier/doi/10.1385/ENDO:15:3:309 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Humana Press |
publisher.none.fl_str_mv |
Humana Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269718227976192 |
score |
13.13397 |