Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance

Autores
Llorente, Briardo; López, Maria G.; Carrari, Fernando Oscar; Asis, Ramón; Di Paola Naranjo, Romina Daniela; Flawia, Mirtha Maria; Alonso, Guillermo Daniel; Bravo Almonacid, Fernando Felix
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Land plants synthesize phenolic compounds involved in plant defense against invading pathogens through the phenylpropanoid pathway. Although not considered as part of the phenylpropanoid pathway, plant polyphenol oxidases (PPOs) are enzymes that catalyze cresolase and catecholase reactions on several phenolic compounds. Here, transgenic potato (Solanum tuberosum) tubers with downregulated PPO genes (-PPO) were challenged with the oomycete pathogen Phytophthora infestans to investigate the interactions between PPO, phenylpropanoid metabolism, and disease resistance. We found that pathogen invasiveness was reduced in -PPO lines, while microscopic evidences suggested that the mechanism underlying the defense response involved the participation of phenolic compounds. Detailed metabolite-profiling analyses demonstrated that the concentration of metabolites related to the phenylpropanoid pathway and chlorogenate in particular was largely altered in PPO-downregulated tubers. Silencing of PPO caused a shift in metabolism from phenylpropanoid precursors to downstream phenylpropanoid products. The presented results suggest that downregulation of PPO redirects the phenylpropanoid metabolism leading to the accumulation of defensive phenolic compounds in the plant cells, consequently enhancing resistance to the pathogen. These results emphasize the importance of components acting in parallel to canonical metabolic pathway constituents in influencing plant metabolism and reveal new scenarios for modulating the levels of phenolics in crops.
Fil: Llorente, Briardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina
Fil: López, Maria G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Di Paola Naranjo, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Flawia, Mirtha Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Materia
Chlorogenate
Host Plant Resistance
Phenylpropanoid Metabolism
Phytophthora Infestans
Polyphenol Oxidase
Solanum Tuberosum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32003
Acceder
id CONICETDig_ded7d667ec8a94b99a14f3c879394d68
oai_identifier_str oai:ri.conicet.gov.ar:11336/32003
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistanceLlorente, BriardoLópez, Maria G.Carrari, Fernando OscarAsis, RamónDi Paola Naranjo, Romina DanielaFlawia, Mirtha MariaAlonso, Guillermo DanielBravo Almonacid, Fernando FelixChlorogenateHost Plant ResistancePhenylpropanoid MetabolismPhytophthora InfestansPolyphenol OxidaseSolanum Tuberosumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Land plants synthesize phenolic compounds involved in plant defense against invading pathogens through the phenylpropanoid pathway. Although not considered as part of the phenylpropanoid pathway, plant polyphenol oxidases (PPOs) are enzymes that catalyze cresolase and catecholase reactions on several phenolic compounds. Here, transgenic potato (Solanum tuberosum) tubers with downregulated PPO genes (-PPO) were challenged with the oomycete pathogen Phytophthora infestans to investigate the interactions between PPO, phenylpropanoid metabolism, and disease resistance. We found that pathogen invasiveness was reduced in -PPO lines, while microscopic evidences suggested that the mechanism underlying the defense response involved the participation of phenolic compounds. Detailed metabolite-profiling analyses demonstrated that the concentration of metabolites related to the phenylpropanoid pathway and chlorogenate in particular was largely altered in PPO-downregulated tubers. Silencing of PPO caused a shift in metabolism from phenylpropanoid precursors to downstream phenylpropanoid products. The presented results suggest that downregulation of PPO redirects the phenylpropanoid metabolism leading to the accumulation of defensive phenolic compounds in the plant cells, consequently enhancing resistance to the pathogen. These results emphasize the importance of components acting in parallel to canonical metabolic pathway constituents in influencing plant metabolism and reveal new scenarios for modulating the levels of phenolics in crops.Fil: Llorente, Briardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; ArgentinaFil: López, Maria G.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Di Paola Naranjo, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Flawia, Mirtha Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32003Bravo Almonacid, Fernando Felix; Alonso, Guillermo Daniel; Flawia, Mirtha Maria; Di Paola Naranjo, Romina Daniela; Asis, Ramón; Carrari, Fernando Oscar; et al.; Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance; Springer; Molecular Breeding; 34; 4; 12-2014; 2049-20631380-3743CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11032-014-0162-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11032-014-0162-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:27Zoai:ri.conicet.gov.ar:11336/32003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:27.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
title Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
spellingShingle Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
Llorente, Briardo
Chlorogenate
Host Plant Resistance
Phenylpropanoid Metabolism
Phytophthora Infestans
Polyphenol Oxidase
Solanum Tuberosum
title_short Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
title_full Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
title_fullStr Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
title_full_unstemmed Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
title_sort Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance
dc.creator.none.fl_str_mv Llorente, Briardo
López, Maria G.
Carrari, Fernando Oscar
Asis, Ramón
Di Paola Naranjo, Romina Daniela
Flawia, Mirtha Maria
Alonso, Guillermo Daniel
Bravo Almonacid, Fernando Felix
author Llorente, Briardo
author_facet Llorente, Briardo
López, Maria G.
Carrari, Fernando Oscar
Asis, Ramón
Di Paola Naranjo, Romina Daniela
Flawia, Mirtha Maria
Alonso, Guillermo Daniel
Bravo Almonacid, Fernando Felix
author_role author
author2 López, Maria G.
Carrari, Fernando Oscar
Asis, Ramón
Di Paola Naranjo, Romina Daniela
Flawia, Mirtha Maria
Alonso, Guillermo Daniel
Bravo Almonacid, Fernando Felix
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chlorogenate
Host Plant Resistance
Phenylpropanoid Metabolism
Phytophthora Infestans
Polyphenol Oxidase
Solanum Tuberosum
topic Chlorogenate
Host Plant Resistance
Phenylpropanoid Metabolism
Phytophthora Infestans
Polyphenol Oxidase
Solanum Tuberosum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Land plants synthesize phenolic compounds involved in plant defense against invading pathogens through the phenylpropanoid pathway. Although not considered as part of the phenylpropanoid pathway, plant polyphenol oxidases (PPOs) are enzymes that catalyze cresolase and catecholase reactions on several phenolic compounds. Here, transgenic potato (Solanum tuberosum) tubers with downregulated PPO genes (-PPO) were challenged with the oomycete pathogen Phytophthora infestans to investigate the interactions between PPO, phenylpropanoid metabolism, and disease resistance. We found that pathogen invasiveness was reduced in -PPO lines, while microscopic evidences suggested that the mechanism underlying the defense response involved the participation of phenolic compounds. Detailed metabolite-profiling analyses demonstrated that the concentration of metabolites related to the phenylpropanoid pathway and chlorogenate in particular was largely altered in PPO-downregulated tubers. Silencing of PPO caused a shift in metabolism from phenylpropanoid precursors to downstream phenylpropanoid products. The presented results suggest that downregulation of PPO redirects the phenylpropanoid metabolism leading to the accumulation of defensive phenolic compounds in the plant cells, consequently enhancing resistance to the pathogen. These results emphasize the importance of components acting in parallel to canonical metabolic pathway constituents in influencing plant metabolism and reveal new scenarios for modulating the levels of phenolics in crops.
Fil: Llorente, Briardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina
Fil: López, Maria G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Di Paola Naranjo, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Flawia, Mirtha Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Alonso, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
description Land plants synthesize phenolic compounds involved in plant defense against invading pathogens through the phenylpropanoid pathway. Although not considered as part of the phenylpropanoid pathway, plant polyphenol oxidases (PPOs) are enzymes that catalyze cresolase and catecholase reactions on several phenolic compounds. Here, transgenic potato (Solanum tuberosum) tubers with downregulated PPO genes (-PPO) were challenged with the oomycete pathogen Phytophthora infestans to investigate the interactions between PPO, phenylpropanoid metabolism, and disease resistance. We found that pathogen invasiveness was reduced in -PPO lines, while microscopic evidences suggested that the mechanism underlying the defense response involved the participation of phenolic compounds. Detailed metabolite-profiling analyses demonstrated that the concentration of metabolites related to the phenylpropanoid pathway and chlorogenate in particular was largely altered in PPO-downregulated tubers. Silencing of PPO caused a shift in metabolism from phenylpropanoid precursors to downstream phenylpropanoid products. The presented results suggest that downregulation of PPO redirects the phenylpropanoid metabolism leading to the accumulation of defensive phenolic compounds in the plant cells, consequently enhancing resistance to the pathogen. These results emphasize the importance of components acting in parallel to canonical metabolic pathway constituents in influencing plant metabolism and reveal new scenarios for modulating the levels of phenolics in crops.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32003
Bravo Almonacid, Fernando Felix; Alonso, Guillermo Daniel; Flawia, Mirtha Maria; Di Paola Naranjo, Romina Daniela; Asis, Ramón; Carrari, Fernando Oscar; et al.; Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance; Springer; Molecular Breeding; 34; 4; 12-2014; 2049-2063
1380-3743
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32003
identifier_str_mv Bravo Almonacid, Fernando Felix; Alonso, Guillermo Daniel; Flawia, Mirtha Maria; Di Paola Naranjo, Romina Daniela; Asis, Ramón; Carrari, Fernando Oscar; et al.; Downregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance; Springer; Molecular Breeding; 34; 4; 12-2014; 2049-2063
1380-3743
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11032-014-0162-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11032-014-0162-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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