Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells

Autores
Martinez, María Julia; Barbini, Luciana; Andreu, Adriana Balbina
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Polyphenols are plant metabolites that have been largely studied for their beneficial effect on human health. Potato is one of the most important crops worldwide and is a relevant source of human dietary nutrients and antioxidants. Particularly, pigmented potatoes contain the highest levels of polyphenolic compounds. Hepatocellular carcinoma is one of the most frequent types of cancers worldwide and despite the existence of treatments; it is yet associated with a high mortality rate. Thus, new drugs are needed, and polyphenols are a potential source of anti-hepatocellular carcinoma compounds. The objectives of this study were to determine the content of different groups of polyphenols (phenolic acids, anthocyanins and flavan-3-ols) in five potato polyphenolic extracts, and to study their antioxidant and cytotoxic activities on a human hepatocellular carcinoma cell line. Methods: four Andean varieties and one industrial potato variety were selected for this study. Polyphenolic quantification and composition were determined by spectrophotometric assays and HPLC-DAD analysis. The antioxidant and cytotoxic activities were evaluated through DPPH and MTS assays respectively. Results showed that pigmented varieties possessed higher levels of the analyzed phenolic compounds. HPLC analysis showed that chlorogenic acid was the main phenolic acid in all the potato polyphenolic extracts. Also, pigmented potatoes presented higher levels of antioxidant activity compared to non-pigmented ones, showing a positive correlation with the total phenolic content. Finally, treatment with three of the studied potato polyphenolic extracts reduced the viability of Hep3B. Furthermore, one extract from a non-pigmented variety affected cell viability to a similar extent as extracts from pigmented potatoes, suggesting that other compounds, besides anthocyanins, may be responsible of the cytotoxic effect of this polyphenolic extracts. Conclusion: These results suggested that polyphenolic compounds present in the Andean potato varieties could be used as a potential source of anti-hepatocarcinoma drugs.
Fil: Martinez, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Barbini, Luciana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
POLYPHENOL EXTRACT
ANDEAN
INDUSTRIAL
SOLANUM TUBEROSUM TUBERS
CYTOTOXIC ACTIVITY
HUMAN HEPATOCARCINOMA CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87410
Acceder
id CONICETDig_69b9e4bfd22010bf1a85f096c8bd9f16
oai_identifier_str oai:ri.conicet.gov.ar:11336/87410
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cellsMartinez, María JuliaBarbini, LucianaAndreu, Adriana BalbinaPOLYPHENOL EXTRACTANDEANINDUSTRIALSOLANUM TUBEROSUM TUBERSCYTOTOXIC ACTIVITYHUMAN HEPATOCARCINOMA CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Polyphenols are plant metabolites that have been largely studied for their beneficial effect on human health. Potato is one of the most important crops worldwide and is a relevant source of human dietary nutrients and antioxidants. Particularly, pigmented potatoes contain the highest levels of polyphenolic compounds. Hepatocellular carcinoma is one of the most frequent types of cancers worldwide and despite the existence of treatments; it is yet associated with a high mortality rate. Thus, new drugs are needed, and polyphenols are a potential source of anti-hepatocellular carcinoma compounds. The objectives of this study were to determine the content of different groups of polyphenols (phenolic acids, anthocyanins and flavan-3-ols) in five potato polyphenolic extracts, and to study their antioxidant and cytotoxic activities on a human hepatocellular carcinoma cell line. Methods: four Andean varieties and one industrial potato variety were selected for this study. Polyphenolic quantification and composition were determined by spectrophotometric assays and HPLC-DAD analysis. The antioxidant and cytotoxic activities were evaluated through DPPH and MTS assays respectively. Results showed that pigmented varieties possessed higher levels of the analyzed phenolic compounds. HPLC analysis showed that chlorogenic acid was the main phenolic acid in all the potato polyphenolic extracts. Also, pigmented potatoes presented higher levels of antioxidant activity compared to non-pigmented ones, showing a positive correlation with the total phenolic content. Finally, treatment with three of the studied potato polyphenolic extracts reduced the viability of Hep3B. Furthermore, one extract from a non-pigmented variety affected cell viability to a similar extent as extracts from pigmented potatoes, suggesting that other compounds, besides anthocyanins, may be responsible of the cytotoxic effect of this polyphenolic extracts. Conclusion: These results suggested that polyphenolic compounds present in the Andean potato varieties could be used as a potential source of anti-hepatocarcinoma drugs.Fil: Martinez, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Barbini, Luciana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaSift Desk Journals2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87410Martinez, María Julia; Barbini, Luciana; Andreu, Adriana Balbina; Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells; Sift Desk Journals; Journal of Food Science & Technology; 8; 74; 1-20182472-6419CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.25177/JFST.2.2.5info:eu-repo/semantics/altIdentifier/url/https://www.siftdesk.org/article-details/Chemical-characterization-of-polyphenol-extracts-from-Andean-and-industrial-Solanum-tuberosum-tubers-and-their-cytotoxic-activity-on-human-hepatocarcinoma-cells/260info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:56Zoai:ri.conicet.gov.ar:11336/87410instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:56.838CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
title Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
spellingShingle Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
Martinez, María Julia
POLYPHENOL EXTRACT
ANDEAN
INDUSTRIAL
SOLANUM TUBEROSUM TUBERS
CYTOTOXIC ACTIVITY
HUMAN HEPATOCARCINOMA CELLS
title_short Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
title_full Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
title_fullStr Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
title_full_unstemmed Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
title_sort Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells
dc.creator.none.fl_str_mv Martinez, María Julia
Barbini, Luciana
Andreu, Adriana Balbina
author Martinez, María Julia
author_facet Martinez, María Julia
Barbini, Luciana
Andreu, Adriana Balbina
author_role author
author2 Barbini, Luciana
Andreu, Adriana Balbina
author2_role author
author
dc.subject.none.fl_str_mv POLYPHENOL EXTRACT
ANDEAN
INDUSTRIAL
SOLANUM TUBEROSUM TUBERS
CYTOTOXIC ACTIVITY
HUMAN HEPATOCARCINOMA CELLS
topic POLYPHENOL EXTRACT
ANDEAN
INDUSTRIAL
SOLANUM TUBEROSUM TUBERS
CYTOTOXIC ACTIVITY
HUMAN HEPATOCARCINOMA CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Polyphenols are plant metabolites that have been largely studied for their beneficial effect on human health. Potato is one of the most important crops worldwide and is a relevant source of human dietary nutrients and antioxidants. Particularly, pigmented potatoes contain the highest levels of polyphenolic compounds. Hepatocellular carcinoma is one of the most frequent types of cancers worldwide and despite the existence of treatments; it is yet associated with a high mortality rate. Thus, new drugs are needed, and polyphenols are a potential source of anti-hepatocellular carcinoma compounds. The objectives of this study were to determine the content of different groups of polyphenols (phenolic acids, anthocyanins and flavan-3-ols) in five potato polyphenolic extracts, and to study their antioxidant and cytotoxic activities on a human hepatocellular carcinoma cell line. Methods: four Andean varieties and one industrial potato variety were selected for this study. Polyphenolic quantification and composition were determined by spectrophotometric assays and HPLC-DAD analysis. The antioxidant and cytotoxic activities were evaluated through DPPH and MTS assays respectively. Results showed that pigmented varieties possessed higher levels of the analyzed phenolic compounds. HPLC analysis showed that chlorogenic acid was the main phenolic acid in all the potato polyphenolic extracts. Also, pigmented potatoes presented higher levels of antioxidant activity compared to non-pigmented ones, showing a positive correlation with the total phenolic content. Finally, treatment with three of the studied potato polyphenolic extracts reduced the viability of Hep3B. Furthermore, one extract from a non-pigmented variety affected cell viability to a similar extent as extracts from pigmented potatoes, suggesting that other compounds, besides anthocyanins, may be responsible of the cytotoxic effect of this polyphenolic extracts. Conclusion: These results suggested that polyphenolic compounds present in the Andean potato varieties could be used as a potential source of anti-hepatocarcinoma drugs.
Fil: Martinez, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Barbini, Luciana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Background: Polyphenols are plant metabolites that have been largely studied for their beneficial effect on human health. Potato is one of the most important crops worldwide and is a relevant source of human dietary nutrients and antioxidants. Particularly, pigmented potatoes contain the highest levels of polyphenolic compounds. Hepatocellular carcinoma is one of the most frequent types of cancers worldwide and despite the existence of treatments; it is yet associated with a high mortality rate. Thus, new drugs are needed, and polyphenols are a potential source of anti-hepatocellular carcinoma compounds. The objectives of this study were to determine the content of different groups of polyphenols (phenolic acids, anthocyanins and flavan-3-ols) in five potato polyphenolic extracts, and to study their antioxidant and cytotoxic activities on a human hepatocellular carcinoma cell line. Methods: four Andean varieties and one industrial potato variety were selected for this study. Polyphenolic quantification and composition were determined by spectrophotometric assays and HPLC-DAD analysis. The antioxidant and cytotoxic activities were evaluated through DPPH and MTS assays respectively. Results showed that pigmented varieties possessed higher levels of the analyzed phenolic compounds. HPLC analysis showed that chlorogenic acid was the main phenolic acid in all the potato polyphenolic extracts. Also, pigmented potatoes presented higher levels of antioxidant activity compared to non-pigmented ones, showing a positive correlation with the total phenolic content. Finally, treatment with three of the studied potato polyphenolic extracts reduced the viability of Hep3B. Furthermore, one extract from a non-pigmented variety affected cell viability to a similar extent as extracts from pigmented potatoes, suggesting that other compounds, besides anthocyanins, may be responsible of the cytotoxic effect of this polyphenolic extracts. Conclusion: These results suggested that polyphenolic compounds present in the Andean potato varieties could be used as a potential source of anti-hepatocarcinoma drugs.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87410
Martinez, María Julia; Barbini, Luciana; Andreu, Adriana Balbina; Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells; Sift Desk Journals; Journal of Food Science & Technology; 8; 74; 1-2018
2472-6419
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87410
identifier_str_mv Martinez, María Julia; Barbini, Luciana; Andreu, Adriana Balbina; Chemical characterization of polyphenol extracts from Andean and industrial Solanum tuberosum tubers and their cytotoxic activity on human hepatocarcinoma cells; Sift Desk Journals; Journal of Food Science & Technology; 8; 74; 1-2018
2472-6419
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.25177/JFST.2.2.5
info:eu-repo/semantics/altIdentifier/url/https://www.siftdesk.org/article-details/Chemical-characterization-of-polyphenol-extracts-from-Andean-and-industrial-Solanum-tuberosum-tubers-and-their-cytotoxic-activity-on-human-hepatocarcinoma-cells/260
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sift Desk Journals
publisher.none.fl_str_mv Sift Desk Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268945800757248
score 13.13397

Publicaciones similares