Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species

Autores
Bossi, Mariano Luis; Folling, Jonas; Belov, Vladimir N.; Boyarskiy, Vadim P.; Medda, Rebecca; Egner, Alexander; Eggeling, Christian; Schonle, Andreas; Hell, Stefan W.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Folling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Boyarskiy, Vadim P.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Schonle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
Materia
multicolor imaging
rhodamines
single molecules
fluorescence nanoscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103124
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103124
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular SpeciesBossi, Mariano LuisFolling, JonasBelov, Vladimir N.Boyarskiy, Vadim P.Medda, RebeccaEgner, AlexanderEggeling, ChristianSchonle, AndreasHell, Stefan W.multicolor imagingrhodaminessingle moleculesfluorescence nanoscopyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Folling, Jonas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Boyarskiy, Vadim P.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Schonle, Andreas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; AlemaniaAmerican Chemical Society2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103124Bossi, Mariano Luis; Folling, Jonas; Belov, Vladimir N.; Boyarskiy, Vadim P.; Medda, Rebecca; et al.; Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species; American Chemical Society; Nano Letters; 8; 8; 8-2008; 2463-24681530-6984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/nl801471dinfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/nl801471dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:44Zoai:ri.conicet.gov.ar:11336/103124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:44.551CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
title Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
spellingShingle Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
Bossi, Mariano Luis
multicolor imaging
rhodamines
single molecules
fluorescence nanoscopy
title_short Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
title_full Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
title_fullStr Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
title_full_unstemmed Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
title_sort Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
dc.creator.none.fl_str_mv Bossi, Mariano Luis
Folling, Jonas
Belov, Vladimir N.
Boyarskiy, Vadim P.
Medda, Rebecca
Egner, Alexander
Eggeling, Christian
Schonle, Andreas
Hell, Stefan W.
author Bossi, Mariano Luis
author_facet Bossi, Mariano Luis
Folling, Jonas
Belov, Vladimir N.
Boyarskiy, Vadim P.
Medda, Rebecca
Egner, Alexander
Eggeling, Christian
Schonle, Andreas
Hell, Stefan W.
author_role author
author2 Folling, Jonas
Belov, Vladimir N.
Boyarskiy, Vadim P.
Medda, Rebecca
Egner, Alexander
Eggeling, Christian
Schonle, Andreas
Hell, Stefan W.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv multicolor imaging
rhodamines
single molecules
fluorescence nanoscopy
topic multicolor imaging
rhodamines
single molecules
fluorescence nanoscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Folling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Boyarskiy, Vadim P.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Schonle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
description By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103124
Bossi, Mariano Luis; Folling, Jonas; Belov, Vladimir N.; Boyarskiy, Vadim P.; Medda, Rebecca; et al.; Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species; American Chemical Society; Nano Letters; 8; 8; 8-2008; 2463-2468
1530-6984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103124
identifier_str_mv Bossi, Mariano Luis; Folling, Jonas; Belov, Vladimir N.; Boyarskiy, Vadim P.; Medda, Rebecca; et al.; Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species; American Chemical Society; Nano Letters; 8; 8; 8-2008; 2463-2468
1530-6984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/nl801471d
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/nl801471d
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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