Fluorescence nanoscopy by ground-state depletion and single-molecule return

Autores
Fölling, Jonas; Bossi, Mariano Luis; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; Hein, Birka; Jakobs, Stefan; Eggeling, Christian; Hell, Stefan W
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bock, Hannes. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Wurm, Christian A. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hein, Birka. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W. Max Planck Institute for Biophysical Chemistry; Alemania
Materia
fluorescence nanoscopy
single molecules
super-resolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103015
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluorescence nanoscopy by ground-state depletion and single-molecule returnFölling, JonasBossi, Mariano LuisBock, HannesMedda, RebeccaWurm, Christian AHein, BirkaJakobs, StefanEggeling, ChristianHell, Stefan Wfluorescence nanoscopysingle moleculessuper-resolutionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bock, Hannes. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Wurm, Christian A. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hein, Birka. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W. Max Planck Institute for Biophysical Chemistry; AlemaniaNature Publishing Group2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103015Fölling, Jonas; Bossi, Mariano Luis; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; et al.; Fluorescence nanoscopy by ground-state depletion and single-molecule return; Nature Publishing Group; Nature Methods; 5; 11; 9-2008; 943-9451548-7091CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/nmeth.1257info:eu-repo/semantics/altIdentifier/doi/10.1038/nmeth.1257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:38Zoai:ri.conicet.gov.ar:11336/103015instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:38.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluorescence nanoscopy by ground-state depletion and single-molecule return
title Fluorescence nanoscopy by ground-state depletion and single-molecule return
spellingShingle Fluorescence nanoscopy by ground-state depletion and single-molecule return
Fölling, Jonas
fluorescence nanoscopy
single molecules
super-resolution
title_short Fluorescence nanoscopy by ground-state depletion and single-molecule return
title_full Fluorescence nanoscopy by ground-state depletion and single-molecule return
title_fullStr Fluorescence nanoscopy by ground-state depletion and single-molecule return
title_full_unstemmed Fluorescence nanoscopy by ground-state depletion and single-molecule return
title_sort Fluorescence nanoscopy by ground-state depletion and single-molecule return
dc.creator.none.fl_str_mv Fölling, Jonas
Bossi, Mariano Luis
Bock, Hannes
Medda, Rebecca
Wurm, Christian A
Hein, Birka
Jakobs, Stefan
Eggeling, Christian
Hell, Stefan W
author Fölling, Jonas
author_facet Fölling, Jonas
Bossi, Mariano Luis
Bock, Hannes
Medda, Rebecca
Wurm, Christian A
Hein, Birka
Jakobs, Stefan
Eggeling, Christian
Hell, Stefan W
author_role author
author2 Bossi, Mariano Luis
Bock, Hannes
Medda, Rebecca
Wurm, Christian A
Hein, Birka
Jakobs, Stefan
Eggeling, Christian
Hell, Stefan W
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv fluorescence nanoscopy
single molecules
super-resolution
topic fluorescence nanoscopy
single molecules
super-resolution
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bock, Hannes. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Wurm, Christian A. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hein, Birka. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W. Max Planck Institute for Biophysical Chemistry; Alemania
description We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103015
Fölling, Jonas; Bossi, Mariano Luis; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; et al.; Fluorescence nanoscopy by ground-state depletion and single-molecule return; Nature Publishing Group; Nature Methods; 5; 11; 9-2008; 943-945
1548-7091
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103015
identifier_str_mv Fölling, Jonas; Bossi, Mariano Luis; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; et al.; Fluorescence nanoscopy by ground-state depletion and single-molecule return; Nature Publishing Group; Nature Methods; 5; 11; 9-2008; 943-945
1548-7091
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/nmeth.1257
info:eu-repo/semantics/altIdentifier/doi/10.1038/nmeth.1257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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