Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

• Autores: Bossi, M.; Föiling, J.; Belov, V.N.; Boyarskiy, V.P.; Medda, R.; Egner, A.; Eggeling, C.; Schönle, A.; Hell, S.W.
• Año de publicación: 2008
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Nano Lett. 2008;8(8):2463-2468
• Materia: Imaging techniques; Mammals; Molecular structure; Optical switches; Biological specimens; Color channels; Far fields; Functional imaging; Mammalian cells; Molecular species; Multicolor imaging; New class; Photoswitching; Single-molecule levels; Spatial resolutions; Switchable; Fluorescence; fluorescent dye; animal; article; cell line; chemical structure; chemistry; color; methodology; microscopy; Animals; Cell Line; Color; Fluorescent Dyes; Microscopy; Molecular Structure
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society.
Fil:Bossi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.