Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets

Autores
Caruso, Benjamin; Wilke, Natalia; Perillo, Maria Angelica
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lipid Droplets (LD) are intracellular structures consisting of an apolar lipid core, composed mainly of triglycerides (TG) and steryl esters, coated by a lipid-protein mixed monolayer. Current models of LD biogenesis assume the presence of nanoscopic TG blisters inside the endoplasmic reticulum (ER) bilayer. However, the mechanism of formation of such initial structure needs further empirical support. Here, we study mixtures of egg-phosphatidylcholine (PC) and TG at the air-water interface in order to gain insight into those factors that affect the stability of TG bulk structures in contact with interfaces. Brewster Angle Microscopy (BAM) imaging revealed the appearance of microscopic Collapsed Structures (CS) at surface pressures (π) beyond collapse, whose lateral size (~1 mm lateral radius) did not vary with composition or lateral packing and behaved as equilibrium and highly stable structures. By Surface Spectral Fluorescence Microscopy (SSFM), we were able to characterize the solvatochromism of Nile Red (NR) both in monolayers and inside CS. This allowed concluding that CS corresponded to a TG liquid phase and to characterize them as lenses forming a three-phase (oil-water-air) system. Then, lenses ́ thickness could be determined, observing that they were dramatically flattened when the coating monolayer contained PC (6-12 nm against 30-50 nm for lenses on PC/TG and TG films, respectively). The shape of lenses were brought together with interfacial tensions into the Neumann ́s triangle, which allowed estimating the oil-water interfacial tension acting at each individual microscopic lens, at varying compression states of the surrounding monolayer. Thus, this model would be useful to evaluate the modulating effects that specific factors could exert on the aggregation of neutral lipids into nascent lipid droplet in the ER, such as endogenous proteins or those associated to pathogens infections.
Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Primeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Biofísica
Materia
Lipid Droplets
Monocapas
Triglicéridos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237828
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid dropletsCaruso, BenjaminWilke, NataliaPerillo, Maria AngelicaLipid DropletsMonocapasTriglicéridoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lipid Droplets (LD) are intracellular structures consisting of an apolar lipid core, composed mainly of triglycerides (TG) and steryl esters, coated by a lipid-protein mixed monolayer. Current models of LD biogenesis assume the presence of nanoscopic TG blisters inside the endoplasmic reticulum (ER) bilayer. However, the mechanism of formation of such initial structure needs further empirical support. Here, we study mixtures of egg-phosphatidylcholine (PC) and TG at the air-water interface in order to gain insight into those factors that affect the stability of TG bulk structures in contact with interfaces. Brewster Angle Microscopy (BAM) imaging revealed the appearance of microscopic Collapsed Structures (CS) at surface pressures (π) beyond collapse, whose lateral size (~1 mm lateral radius) did not vary with composition or lateral packing and behaved as equilibrium and highly stable structures. By Surface Spectral Fluorescence Microscopy (SSFM), we were able to characterize the solvatochromism of Nile Red (NR) both in monolayers and inside CS. This allowed concluding that CS corresponded to a TG liquid phase and to characterize them as lenses forming a three-phase (oil-water-air) system. Then, lenses ́ thickness could be determined, observing that they were dramatically flattened when the coating monolayer contained PC (6-12 nm against 30-50 nm for lenses on PC/TG and TG films, respectively). The shape of lenses were brought together with interfacial tensions into the Neumann ́s triangle, which allowed estimating the oil-water interfacial tension acting at each individual microscopic lens, at varying compression states of the surrounding monolayer. Thus, this model would be useful to evaluate the modulating effects that specific factors could exert on the aggregation of neutral lipids into nascent lipid droplet in the ER, such as endogenous proteins or those associated to pathogens infections.Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaPrimeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19Ciudad Autónoma de Buenos AiresArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAmbroggio, Ernesto EstebanCelej, Maria SoledadHollmann, AxelAcierno, Juan Pablo2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237828Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets; Primeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 36-36978-987-27591-8-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:02Zoai:ri.conicet.gov.ar:11336/237828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:03.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
title Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
spellingShingle Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
Caruso, Benjamin
Lipid Droplets
Monocapas
Triglicéridos
title_short Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
title_full Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
title_fullStr Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
title_full_unstemmed Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
title_sort Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets
dc.creator.none.fl_str_mv Caruso, Benjamin
Wilke, Natalia
Perillo, Maria Angelica
author Caruso, Benjamin
author_facet Caruso, Benjamin
Wilke, Natalia
Perillo, Maria Angelica
author_role author
author2 Wilke, Natalia
Perillo, Maria Angelica
author2_role author
author
dc.contributor.none.fl_str_mv Ambroggio, Ernesto Esteban
Celej, Maria Soledad
Hollmann, Axel
Acierno, Juan Pablo
dc.subject.none.fl_str_mv Lipid Droplets
Monocapas
Triglicéridos
topic Lipid Droplets
Monocapas
Triglicéridos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lipid Droplets (LD) are intracellular structures consisting of an apolar lipid core, composed mainly of triglycerides (TG) and steryl esters, coated by a lipid-protein mixed monolayer. Current models of LD biogenesis assume the presence of nanoscopic TG blisters inside the endoplasmic reticulum (ER) bilayer. However, the mechanism of formation of such initial structure needs further empirical support. Here, we study mixtures of egg-phosphatidylcholine (PC) and TG at the air-water interface in order to gain insight into those factors that affect the stability of TG bulk structures in contact with interfaces. Brewster Angle Microscopy (BAM) imaging revealed the appearance of microscopic Collapsed Structures (CS) at surface pressures (π) beyond collapse, whose lateral size (~1 mm lateral radius) did not vary with composition or lateral packing and behaved as equilibrium and highly stable structures. By Surface Spectral Fluorescence Microscopy (SSFM), we were able to characterize the solvatochromism of Nile Red (NR) both in monolayers and inside CS. This allowed concluding that CS corresponded to a TG liquid phase and to characterize them as lenses forming a three-phase (oil-water-air) system. Then, lenses ́ thickness could be determined, observing that they were dramatically flattened when the coating monolayer contained PC (6-12 nm against 30-50 nm for lenses on PC/TG and TG films, respectively). The shape of lenses were brought together with interfacial tensions into the Neumann ́s triangle, which allowed estimating the oil-water interfacial tension acting at each individual microscopic lens, at varying compression states of the surrounding monolayer. Thus, this model would be useful to evaluate the modulating effects that specific factors could exert on the aggregation of neutral lipids into nascent lipid droplet in the ER, such as endogenous proteins or those associated to pathogens infections.
Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Primeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Biofísica
description Lipid Droplets (LD) are intracellular structures consisting of an apolar lipid core, composed mainly of triglycerides (TG) and steryl esters, coated by a lipid-protein mixed monolayer. Current models of LD biogenesis assume the presence of nanoscopic TG blisters inside the endoplasmic reticulum (ER) bilayer. However, the mechanism of formation of such initial structure needs further empirical support. Here, we study mixtures of egg-phosphatidylcholine (PC) and TG at the air-water interface in order to gain insight into those factors that affect the stability of TG bulk structures in contact with interfaces. Brewster Angle Microscopy (BAM) imaging revealed the appearance of microscopic Collapsed Structures (CS) at surface pressures (π) beyond collapse, whose lateral size (~1 mm lateral radius) did not vary with composition or lateral packing and behaved as equilibrium and highly stable structures. By Surface Spectral Fluorescence Microscopy (SSFM), we were able to characterize the solvatochromism of Nile Red (NR) both in monolayers and inside CS. This allowed concluding that CS corresponded to a TG liquid phase and to characterize them as lenses forming a three-phase (oil-water-air) system. Then, lenses ́ thickness could be determined, observing that they were dramatically flattened when the coating monolayer contained PC (6-12 nm against 30-50 nm for lenses on PC/TG and TG films, respectively). The shape of lenses were brought together with interfacial tensions into the Neumann ́s triangle, which allowed estimating the oil-water interfacial tension acting at each individual microscopic lens, at varying compression states of the surrounding monolayer. Thus, this model would be useful to evaluate the modulating effects that specific factors could exert on the aggregation of neutral lipids into nascent lipid droplet in the ER, such as endogenous proteins or those associated to pathogens infections.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237828
Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets; Primeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 36-36
978-987-27591-8-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237828
identifier_str_mv Triglyceride lenses at the air-water interface as a tool for studying the biogenesis of lipid droplets; Primeras Jornadas Virtuales SAB: Biofísica en tiempos de COVID-19; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 36-36
978-987-27591-8-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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