Analysis of lipid droplets in hepatocytes

Autores
Wang, Huajin; Quiroga, Ariel Dario; Lehner, Richard
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract The liver plays an important role in triacylglycerol (TG) metabolism. It can store large amounts of TG in cytosolic lipid droplets (CLDs), or it can package TG into very-low density lipoproteins (VLDL) that are secreted from the cell. TG packaged into VLDL is derived from TG stored within the endoplasmic reticulum in lumenal lipid droplets (LLDs). Therefore, the liver contains at least three kinds of LDs that differ in their protein composition, subcellular localization, and function. Hepatic LDs undergo tremendous changes in their size and protein composition depending on the energetic (fasting/feeding) and pathological (viral infection, nonalcoholic fatty liver disease, etc.) states. It is crucial to develop methodologies that allow the isolation and analyses of the various hepatic LDs in order to gain insight into the differential metabolism of these important lipid storage/transport particles in health and disease. Here, we present detailed protocols for the isolation and analysis of CLDs and LLDs and for monitoring CLD dynamics.
Fil: Wang, Huajin. University Of Yale. Yale School Of Forestry & Environmental Studies; Estados Unidos
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Lehner, Richard. University Of Alberta; Canadá
Materia
Cytosolic Lipid Droplets
Lumenal Lipid Droplets
Bodipy Fatty Acids
Subcellular Fractionation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6035
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of lipid droplets in hepatocytesWang, HuajinQuiroga, Ariel DarioLehner, RichardCytosolic Lipid DropletsLumenal Lipid DropletsBodipy Fatty AcidsSubcellular Fractionationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Abstract The liver plays an important role in triacylglycerol (TG) metabolism. It can store large amounts of TG in cytosolic lipid droplets (CLDs), or it can package TG into very-low density lipoproteins (VLDL) that are secreted from the cell. TG packaged into VLDL is derived from TG stored within the endoplasmic reticulum in lumenal lipid droplets (LLDs). Therefore, the liver contains at least three kinds of LDs that differ in their protein composition, subcellular localization, and function. Hepatic LDs undergo tremendous changes in their size and protein composition depending on the energetic (fasting/feeding) and pathological (viral infection, nonalcoholic fatty liver disease, etc.) states. It is crucial to develop methodologies that allow the isolation and analyses of the various hepatic LDs in order to gain insight into the differential metabolism of these important lipid storage/transport particles in health and disease. Here, we present detailed protocols for the isolation and analysis of CLDs and LLDs and for monitoring CLD dynamics.Fil: Wang, Huajin. University Of Yale. Yale School Of Forestry & Environmental Studies; Estados UnidosFil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Lehner, Richard. University Of Alberta; CanadáElsevier2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6035Wang, Huajin; Quiroga, Ariel Dario; Lehner, Richard; Analysis of lipid droplets in hepatocytes; Elsevier; Methods In Cell Biology; 116; 10-2013; 107-1270091-679Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-408051-5.00007-3info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/B9780124080515000073info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:34Zoai:ri.conicet.gov.ar:11336/6035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:34.965CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of lipid droplets in hepatocytes
title Analysis of lipid droplets in hepatocytes
spellingShingle Analysis of lipid droplets in hepatocytes
Wang, Huajin
Cytosolic Lipid Droplets
Lumenal Lipid Droplets
Bodipy Fatty Acids
Subcellular Fractionation
title_short Analysis of lipid droplets in hepatocytes
title_full Analysis of lipid droplets in hepatocytes
title_fullStr Analysis of lipid droplets in hepatocytes
title_full_unstemmed Analysis of lipid droplets in hepatocytes
title_sort Analysis of lipid droplets in hepatocytes
dc.creator.none.fl_str_mv Wang, Huajin
Quiroga, Ariel Dario
Lehner, Richard
author Wang, Huajin
author_facet Wang, Huajin
Quiroga, Ariel Dario
Lehner, Richard
author_role author
author2 Quiroga, Ariel Dario
Lehner, Richard
author2_role author
author
dc.subject.none.fl_str_mv Cytosolic Lipid Droplets
Lumenal Lipid Droplets
Bodipy Fatty Acids
Subcellular Fractionation
topic Cytosolic Lipid Droplets
Lumenal Lipid Droplets
Bodipy Fatty Acids
Subcellular Fractionation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Abstract The liver plays an important role in triacylglycerol (TG) metabolism. It can store large amounts of TG in cytosolic lipid droplets (CLDs), or it can package TG into very-low density lipoproteins (VLDL) that are secreted from the cell. TG packaged into VLDL is derived from TG stored within the endoplasmic reticulum in lumenal lipid droplets (LLDs). Therefore, the liver contains at least three kinds of LDs that differ in their protein composition, subcellular localization, and function. Hepatic LDs undergo tremendous changes in their size and protein composition depending on the energetic (fasting/feeding) and pathological (viral infection, nonalcoholic fatty liver disease, etc.) states. It is crucial to develop methodologies that allow the isolation and analyses of the various hepatic LDs in order to gain insight into the differential metabolism of these important lipid storage/transport particles in health and disease. Here, we present detailed protocols for the isolation and analysis of CLDs and LLDs and for monitoring CLD dynamics.
Fil: Wang, Huajin. University Of Yale. Yale School Of Forestry & Environmental Studies; Estados Unidos
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Lehner, Richard. University Of Alberta; Canadá
description Abstract The liver plays an important role in triacylglycerol (TG) metabolism. It can store large amounts of TG in cytosolic lipid droplets (CLDs), or it can package TG into very-low density lipoproteins (VLDL) that are secreted from the cell. TG packaged into VLDL is derived from TG stored within the endoplasmic reticulum in lumenal lipid droplets (LLDs). Therefore, the liver contains at least three kinds of LDs that differ in their protein composition, subcellular localization, and function. Hepatic LDs undergo tremendous changes in their size and protein composition depending on the energetic (fasting/feeding) and pathological (viral infection, nonalcoholic fatty liver disease, etc.) states. It is crucial to develop methodologies that allow the isolation and analyses of the various hepatic LDs in order to gain insight into the differential metabolism of these important lipid storage/transport particles in health and disease. Here, we present detailed protocols for the isolation and analysis of CLDs and LLDs and for monitoring CLD dynamics.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6035
Wang, Huajin; Quiroga, Ariel Dario; Lehner, Richard; Analysis of lipid droplets in hepatocytes; Elsevier; Methods In Cell Biology; 116; 10-2013; 107-127
0091-679X
url http://hdl.handle.net/11336/6035
identifier_str_mv Wang, Huajin; Quiroga, Ariel Dario; Lehner, Richard; Analysis of lipid droplets in hepatocytes; Elsevier; Methods In Cell Biology; 116; 10-2013; 107-127
0091-679X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-12-408051-5.00007-3
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/B9780124080515000073
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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