Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system

Autores
Caruso, Benjamin; Tohmé Chapini, María Julieta; Wilke, Natalia; Delgui, Laura Ruth
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.
Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XLVIII Reunion Anual de la Sociedad Argentina de Biofisica
San Luis
Argentina
Sociedad Argentina de Biofísica
Materia
Ursolic Acid
Lipid Droplets
Langmuir Films
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228602
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228602
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model systemCaruso, BenjaminTohmé Chapini, María JulietaWilke, NataliaDelgui, Laura RuthUrsolic AcidLipid DropletsLangmuir Filmshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaXLVIII Reunion Anual de la Sociedad Argentina de BiofisicaSan LuisArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAndujar, Sebastian AntonioCelej, Maria SoledadAcierno, Juan Pablo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228602Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79978-987-27591-7-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:00Zoai:ri.conicet.gov.ar:11336/228602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:00.842CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
title Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
spellingShingle Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
Caruso, Benjamin
Ursolic Acid
Lipid Droplets
Langmuir Films
title_short Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
title_full Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
title_fullStr Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
title_full_unstemmed Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
title_sort Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
dc.creator.none.fl_str_mv Caruso, Benjamin
Tohmé Chapini, María Julieta
Wilke, Natalia
Delgui, Laura Ruth
author Caruso, Benjamin
author_facet Caruso, Benjamin
Tohmé Chapini, María Julieta
Wilke, Natalia
Delgui, Laura Ruth
author_role author
author2 Tohmé Chapini, María Julieta
Wilke, Natalia
Delgui, Laura Ruth
author2_role author
author
author
dc.contributor.none.fl_str_mv Andujar, Sebastian Antonio
Celej, Maria Soledad
Acierno, Juan Pablo
dc.subject.none.fl_str_mv Ursolic Acid
Lipid Droplets
Langmuir Films
topic Ursolic Acid
Lipid Droplets
Langmuir Films
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.
Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XLVIII Reunion Anual de la Sociedad Argentina de Biofisica
San Luis
Argentina
Sociedad Argentina de Biofísica
description According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228602
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79
978-987-27591-7-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228602
identifier_str_mv Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79
978-987-27591-7-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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