Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system
- Autores
- Caruso, Benjamin; Tohmé Chapini, María Julieta; Wilke, Natalia; Delgui, Laura Ruth
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.
Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XLVIII Reunion Anual de la Sociedad Argentina de Biofisica
San Luis
Argentina
Sociedad Argentina de Biofísica - Materia
-
Ursolic Acid
Lipid Droplets
Langmuir Films - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/228602
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Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model systemCaruso, BenjaminTohmé Chapini, María JulietaWilke, NataliaDelgui, Laura RuthUrsolic AcidLipid DropletsLangmuir Filmshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii.Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaXLVIII Reunion Anual de la Sociedad Argentina de BiofisicaSan LuisArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAndujar, Sebastian AntonioCelej, Maria SoledadAcierno, Juan Pablo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228602Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79978-987-27591-7-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:59Zoai:ri.conicet.gov.ar:11336/228602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:59.995CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
title |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
spellingShingle |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system Caruso, Benjamin Ursolic Acid Lipid Droplets Langmuir Films |
title_short |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
title_full |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
title_fullStr |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
title_full_unstemmed |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
title_sort |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system |
dc.creator.none.fl_str_mv |
Caruso, Benjamin Tohmé Chapini, María Julieta Wilke, Natalia Delgui, Laura Ruth |
author |
Caruso, Benjamin |
author_facet |
Caruso, Benjamin Tohmé Chapini, María Julieta Wilke, Natalia Delgui, Laura Ruth |
author_role |
author |
author2 |
Tohmé Chapini, María Julieta Wilke, Natalia Delgui, Laura Ruth |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Andujar, Sebastian Antonio Celej, Maria Soledad Acierno, Juan Pablo |
dc.subject.none.fl_str_mv |
Ursolic Acid Lipid Droplets Langmuir Films |
topic |
Ursolic Acid Lipid Droplets Langmuir Films |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii. Fil: Caruso, Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Wilke, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina XLVIII Reunion Anual de la Sociedad Argentina de Biofisica San Luis Argentina Sociedad Argentina de Biofísica |
description |
According to the latest Lipid Droplet (LD) biogenesis models, interfacial tension plays a critical role on such process, determining the shape of the budding LD from the ER. We have previously presented triglyceride (TG) “lenses” at the air-water interface as a model system for studying LD biogenesis. The performance of Langmuir monolayers of mixed phosphatidylcholine (EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface) and the characterization of their lenses´ thickness by Brewster Angle Microscopy (BAM) allow evaluating the proneness of TG lenses to form a LD. This is done by characterizing the associated thermodynamics and the resulting shape of the TG lenses. Here, we evaluated the modulation of TG lenses tendency to detach from the interface by the presence of a third component with interfacial activity. For this purpose, we choose Ursolic Acid (UA), a natural pentacyclic triterpenoid with many biological effects and a clear interfacial activity. We found that UA slightly diminished the surface pressure at which TG phase separation occurs (i.e., lenses appearance) in composition dependent manner (both %UA and PC/TG relation). Such effect may be correlated with the dependency of UA interfacial partitioning on lipid composition. On the other hand, BAM analysis showed a lower reflectivity of lenses in the presence of UA. Assuming a negligible effect of UA on the refractive index of TG lenses, this implies a thinning of such structures. Additionally, the decreased radii of lenses observed allowed us concluding that the contact angle of the lenses is lower in the presence of UA. In conclusion, this experimental model allowed the detection of an interfacial active compound, like UA, affecting both the energy necessary to form the TG lenses and its shape, leading to structures of lower curvature and radii. These results are in accordance with observed effects of UA on LD in cells: a decrease in LD number and radii. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/228602 Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79 978-987-27591-7-9 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/228602 |
identifier_str_mv |
Assesing the effect of Ursolic Acid on Lipid Droplet biogenesis using a Langmuir model system; XLVIII Reunion Anual de la Sociedad Argentina de Biofisica; San Luis; Argentina; 2019; 79-79 978-987-27591-7-9 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Internacional |
dc.publisher.none.fl_str_mv |
Sociedad Argentina de Biofísica |
publisher.none.fl_str_mv |
Sociedad Argentina de Biofísica |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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