Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles

Autores
Sánchez, Gabriel Antonio; Takara, Delia; Toma, A. F.; Alonso, Guillermo Luis
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We compared the sarcoplasmic reticulum (SR) Ca-ATPase from masseter (M) and medial pterygoid (MP) muscles with that from fast muscles (FM) to examine whether its calcium transport capability and enzymatic activity are different. SR vesicles from FM, M, and MP muscles were obtained according to Champeil et al.(1985). Assays for characterization of the enzyme properties were performed. The results showed similar optimal conditions for the Ca-ATPase activity and calcium transport in M, MP, and FM. However, the maximal values of calcium transport, Ca-ATPase activity, and Ki for thapsigargin were significantly lower in the masticatory muscles. These findings are likely related to different Ca-ATPase isoforms. Since the local anesthetics used in dentistry inhibit Ca-ATPase and calcium transport in FM, it will be important for the effects of these drugs on the Ca-ATPase of masticatory muscles to be assessed.
Fil: Sánchez, Gabriel Antonio. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Takara, Delia. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Toma, A. F.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Materia
SR Ca-ATPase
MASTICATORY MUSCLES
CALCIUM TRANSPORTt
THAPSIGARGIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/251024
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory MusclesSánchez, Gabriel AntonioTakara, DeliaToma, A. F.Alonso, Guillermo LuisSR Ca-ATPaseMASTICATORY MUSCLESCALCIUM TRANSPORTtTHAPSIGARGINhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We compared the sarcoplasmic reticulum (SR) Ca-ATPase from masseter (M) and medial pterygoid (MP) muscles with that from fast muscles (FM) to examine whether its calcium transport capability and enzymatic activity are different. SR vesicles from FM, M, and MP muscles were obtained according to Champeil et al.(1985). Assays for characterization of the enzyme properties were performed. The results showed similar optimal conditions for the Ca-ATPase activity and calcium transport in M, MP, and FM. However, the maximal values of calcium transport, Ca-ATPase activity, and Ki for thapsigargin were significantly lower in the masticatory muscles. These findings are likely related to different Ca-ATPase isoforms. Since the local anesthetics used in dentistry inhibit Ca-ATPase and calcium transport in FM, it will be important for the effects of these drugs on the Ca-ATPase of masticatory muscles to be assessed.Fil: Sánchez, Gabriel Antonio. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaFil: Takara, Delia. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaFil: Toma, A. F.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaFil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaSAGE Publications2004-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/251024Sánchez, Gabriel Antonio; Takara, Delia; Toma, A. F.; Alonso, Guillermo Luis; Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles; SAGE Publications; Journal Of Dental Research; 83; 7; 7-2004; 557-5610022-0345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/154405910408300709info:eu-repo/semantics/altIdentifier/doi/10.1177/154405910408300709info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:11Zoai:ri.conicet.gov.ar:11336/251024instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:12.028CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
title Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
spellingShingle Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
Sánchez, Gabriel Antonio
SR Ca-ATPase
MASTICATORY MUSCLES
CALCIUM TRANSPORTt
THAPSIGARGIN
title_short Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
title_full Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
title_fullStr Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
title_full_unstemmed Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
title_sort Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles
dc.creator.none.fl_str_mv Sánchez, Gabriel Antonio
Takara, Delia
Toma, A. F.
Alonso, Guillermo Luis
author Sánchez, Gabriel Antonio
author_facet Sánchez, Gabriel Antonio
Takara, Delia
Toma, A. F.
Alonso, Guillermo Luis
author_role author
author2 Takara, Delia
Toma, A. F.
Alonso, Guillermo Luis
author2_role author
author
author
dc.subject.none.fl_str_mv SR Ca-ATPase
MASTICATORY MUSCLES
CALCIUM TRANSPORTt
THAPSIGARGIN
topic SR Ca-ATPase
MASTICATORY MUSCLES
CALCIUM TRANSPORTt
THAPSIGARGIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We compared the sarcoplasmic reticulum (SR) Ca-ATPase from masseter (M) and medial pterygoid (MP) muscles with that from fast muscles (FM) to examine whether its calcium transport capability and enzymatic activity are different. SR vesicles from FM, M, and MP muscles were obtained according to Champeil et al.(1985). Assays for characterization of the enzyme properties were performed. The results showed similar optimal conditions for the Ca-ATPase activity and calcium transport in M, MP, and FM. However, the maximal values of calcium transport, Ca-ATPase activity, and Ki for thapsigargin were significantly lower in the masticatory muscles. These findings are likely related to different Ca-ATPase isoforms. Since the local anesthetics used in dentistry inhibit Ca-ATPase and calcium transport in FM, it will be important for the effects of these drugs on the Ca-ATPase of masticatory muscles to be assessed.
Fil: Sánchez, Gabriel Antonio. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Takara, Delia. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Toma, A. F.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
description We compared the sarcoplasmic reticulum (SR) Ca-ATPase from masseter (M) and medial pterygoid (MP) muscles with that from fast muscles (FM) to examine whether its calcium transport capability and enzymatic activity are different. SR vesicles from FM, M, and MP muscles were obtained according to Champeil et al.(1985). Assays for characterization of the enzyme properties were performed. The results showed similar optimal conditions for the Ca-ATPase activity and calcium transport in M, MP, and FM. However, the maximal values of calcium transport, Ca-ATPase activity, and Ki for thapsigargin were significantly lower in the masticatory muscles. These findings are likely related to different Ca-ATPase isoforms. Since the local anesthetics used in dentistry inhibit Ca-ATPase and calcium transport in FM, it will be important for the effects of these drugs on the Ca-ATPase of masticatory muscles to be assessed.
publishDate 2004
dc.date.none.fl_str_mv 2004-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/251024
Sánchez, Gabriel Antonio; Takara, Delia; Toma, A. F.; Alonso, Guillermo Luis; Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles; SAGE Publications; Journal Of Dental Research; 83; 7; 7-2004; 557-561
0022-0345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/251024
identifier_str_mv Sánchez, Gabriel Antonio; Takara, Delia; Toma, A. F.; Alonso, Guillermo Luis; Characteristics of the Sarcoplasmic Reticulum Ca 2+ -dependent ATPase from Masticatory Muscles; SAGE Publications; Journal Of Dental Research; 83; 7; 7-2004; 557-561
0022-0345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/154405910408300709
info:eu-repo/semantics/altIdentifier/doi/10.1177/154405910408300709
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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