Local anesthetics inhibit Ca-ATPase in masticatory muscles

Autores
Sanchez, Gabriel; Takara, Delia; Alonso, Guillermo Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Local anesthetics have myotoxic effects and inhibit Ca-ATPase activity and Ca transport in skeletal muscles. Such effects have not been fully elucidated in masticatory muscles. We tested the hypothesis that local anesthetics increase myoplasmic calcium in masticatory muscles by inhibiting Ca-ATPase at a concentration similar to that of dental cartridges. The effects of lidocaine and bupivacaine on Ca-ATPase from rabbit masseter and medial pterygoid muscles were tested with radioisotopic and colorimetric methods. Bupivacaine had an action similar to that of lidocaine on Ca-ATPase activity, but less effect on calcium transport. The pre-exposure of the membranes to the anesthetics enhanced the Ca-ATPase activity in the absence of calcium ionophore, supporting their permeabilizing effect. The results demonstrate that amide-type anesthetics do not inhibit calcium binding, but do reduce calcium transport and enzyme phosphorylation by ATP, and suggest that the myoplasmic calcium increase induced by lidocaine and bupivacaine might promote masticatory muscle contraction and eventual rigidity.
Fil: Sanchez, Gabriel. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Takara, Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Materia
Bupivacaine.
Lidocaine
Local Anesthetics
Masticatory Muscles
Serca
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67276
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Local anesthetics inhibit Ca-ATPase in masticatory musclesSanchez, GabrielTakara, DeliaAlonso, Guillermo LuisBupivacaine.LidocaineLocal AnestheticsMasticatory MusclesSercahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Local anesthetics have myotoxic effects and inhibit Ca-ATPase activity and Ca transport in skeletal muscles. Such effects have not been fully elucidated in masticatory muscles. We tested the hypothesis that local anesthetics increase myoplasmic calcium in masticatory muscles by inhibiting Ca-ATPase at a concentration similar to that of dental cartridges. The effects of lidocaine and bupivacaine on Ca-ATPase from rabbit masseter and medial pterygoid muscles were tested with radioisotopic and colorimetric methods. Bupivacaine had an action similar to that of lidocaine on Ca-ATPase activity, but less effect on calcium transport. The pre-exposure of the membranes to the anesthetics enhanced the Ca-ATPase activity in the absence of calcium ionophore, supporting their permeabilizing effect. The results demonstrate that amide-type anesthetics do not inhibit calcium binding, but do reduce calcium transport and enzyme phosphorylation by ATP, and suggest that the myoplasmic calcium increase induced by lidocaine and bupivacaine might promote masticatory muscle contraction and eventual rigidity.Fil: Sanchez, Gabriel. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaFil: Takara, Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaFil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; ArgentinaSAGE Publications2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67276Sanchez, Gabriel; Takara, Delia; Alonso, Guillermo Luis; Local anesthetics inhibit Ca-ATPase in masticatory muscles; SAGE Publications; Journal Of Dental Research; 89; 4; 4-2010; 372-3770022-0345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/0022034510363220info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/0022034510363220info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:42Zoai:ri.conicet.gov.ar:11336/67276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:42.38CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Local anesthetics inhibit Ca-ATPase in masticatory muscles
title Local anesthetics inhibit Ca-ATPase in masticatory muscles
spellingShingle Local anesthetics inhibit Ca-ATPase in masticatory muscles
Sanchez, Gabriel
Bupivacaine.
Lidocaine
Local Anesthetics
Masticatory Muscles
Serca
title_short Local anesthetics inhibit Ca-ATPase in masticatory muscles
title_full Local anesthetics inhibit Ca-ATPase in masticatory muscles
title_fullStr Local anesthetics inhibit Ca-ATPase in masticatory muscles
title_full_unstemmed Local anesthetics inhibit Ca-ATPase in masticatory muscles
title_sort Local anesthetics inhibit Ca-ATPase in masticatory muscles
dc.creator.none.fl_str_mv Sanchez, Gabriel
Takara, Delia
Alonso, Guillermo Luis
author Sanchez, Gabriel
author_facet Sanchez, Gabriel
Takara, Delia
Alonso, Guillermo Luis
author_role author
author2 Takara, Delia
Alonso, Guillermo Luis
author2_role author
author
dc.subject.none.fl_str_mv Bupivacaine.
Lidocaine
Local Anesthetics
Masticatory Muscles
Serca
topic Bupivacaine.
Lidocaine
Local Anesthetics
Masticatory Muscles
Serca
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Local anesthetics have myotoxic effects and inhibit Ca-ATPase activity and Ca transport in skeletal muscles. Such effects have not been fully elucidated in masticatory muscles. We tested the hypothesis that local anesthetics increase myoplasmic calcium in masticatory muscles by inhibiting Ca-ATPase at a concentration similar to that of dental cartridges. The effects of lidocaine and bupivacaine on Ca-ATPase from rabbit masseter and medial pterygoid muscles were tested with radioisotopic and colorimetric methods. Bupivacaine had an action similar to that of lidocaine on Ca-ATPase activity, but less effect on calcium transport. The pre-exposure of the membranes to the anesthetics enhanced the Ca-ATPase activity in the absence of calcium ionophore, supporting their permeabilizing effect. The results demonstrate that amide-type anesthetics do not inhibit calcium binding, but do reduce calcium transport and enzyme phosphorylation by ATP, and suggest that the myoplasmic calcium increase induced by lidocaine and bupivacaine might promote masticatory muscle contraction and eventual rigidity.
Fil: Sanchez, Gabriel. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Takara, Delia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
Fil: Alonso, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentina
description Local anesthetics have myotoxic effects and inhibit Ca-ATPase activity and Ca transport in skeletal muscles. Such effects have not been fully elucidated in masticatory muscles. We tested the hypothesis that local anesthetics increase myoplasmic calcium in masticatory muscles by inhibiting Ca-ATPase at a concentration similar to that of dental cartridges. The effects of lidocaine and bupivacaine on Ca-ATPase from rabbit masseter and medial pterygoid muscles were tested with radioisotopic and colorimetric methods. Bupivacaine had an action similar to that of lidocaine on Ca-ATPase activity, but less effect on calcium transport. The pre-exposure of the membranes to the anesthetics enhanced the Ca-ATPase activity in the absence of calcium ionophore, supporting their permeabilizing effect. The results demonstrate that amide-type anesthetics do not inhibit calcium binding, but do reduce calcium transport and enzyme phosphorylation by ATP, and suggest that the myoplasmic calcium increase induced by lidocaine and bupivacaine might promote masticatory muscle contraction and eventual rigidity.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67276
Sanchez, Gabriel; Takara, Delia; Alonso, Guillermo Luis; Local anesthetics inhibit Ca-ATPase in masticatory muscles; SAGE Publications; Journal Of Dental Research; 89; 4; 4-2010; 372-377
0022-0345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67276
identifier_str_mv Sanchez, Gabriel; Takara, Delia; Alonso, Guillermo Luis; Local anesthetics inhibit Ca-ATPase in masticatory muscles; SAGE Publications; Journal Of Dental Research; 89; 4; 4-2010; 372-377
0022-0345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/0022034510363220
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/0022034510363220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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