Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity

Autores
Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to environmental salinity of the coexistent Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities were studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+ ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemide-insensitive and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed Michaelis-Menten kinetics (Km = 0.021 and 0.224 mM, respectively). These characteristics appeared to be quite different from those previously described for Na+-K+ ATPase activity in chela muscle of this crab. Na+-K+ ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10‰), a salinity at which N. granulata exhibits a strong hyperregulatory capacity, Na+-K+ ATPase activity was higher (117 ± 26 nmol Pi min- 1 mg prot- 1) than in 35‰ salinity (23 ± 6 nmol Pi min- 1 mg prot- 1) (a salinity at which this crab is osmoionoconforming). On the contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35‰ salinity (108 ±15 nmol Pi min- 1 mg prot- 1) than in crabs acclimated to 10‰ salinity (36 ± 11 nmol Pi min- 1 mg prot- 1). Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to low salinity. The response to low salinity suggests that Na+-K+ ATPase could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive, furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible differential functional roles of Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are discussed.
Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Materia
CRABS
MUSCLE
NA+ ATPASES
NEOHELICE GRANULATA
OSMOIONOREGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160062

id CONICETDig_bf9f410351bda52b1e9033843df93ef8
oai_identifier_str oai:ri.conicet.gov.ar:11336/160062
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinityPinoni, Silvina AndreaLopez Mañanes, Alejandra AntoniaCRABSMUSCLENA+ ATPASESNEOHELICE GRANULATAOSMOIONOREGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to environmental salinity of the coexistent Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities were studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+ ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemide-insensitive and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed Michaelis-Menten kinetics (Km = 0.021 and 0.224 mM, respectively). These characteristics appeared to be quite different from those previously described for Na+-K+ ATPase activity in chela muscle of this crab. Na+-K+ ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10‰), a salinity at which N. granulata exhibits a strong hyperregulatory capacity, Na+-K+ ATPase activity was higher (117 ± 26 nmol Pi min- 1 mg prot- 1) than in 35‰ salinity (23 ± 6 nmol Pi min- 1 mg prot- 1) (a salinity at which this crab is osmoionoconforming). On the contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35‰ salinity (108 ±15 nmol Pi min- 1 mg prot- 1) than in crabs acclimated to 10‰ salinity (36 ± 11 nmol Pi min- 1 mg prot- 1). Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to low salinity. The response to low salinity suggests that Na+-K+ ATPase could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive, furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible differential functional roles of Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are discussed.Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaElsevier Science2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160062Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 372; 1-2; 4-2009; 91-970022-0981CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0022098109000914info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2009.02.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:38Zoai:ri.conicet.gov.ar:11336/160062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:39.171CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
title Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
spellingShingle Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
Pinoni, Silvina Andrea
CRABS
MUSCLE
NA+ ATPASES
NEOHELICE GRANULATA
OSMOIONOREGULATION
title_short Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
title_full Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
title_fullStr Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
title_full_unstemmed Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
title_sort Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
dc.creator.none.fl_str_mv Pinoni, Silvina Andrea
Lopez Mañanes, Alejandra Antonia
author Pinoni, Silvina Andrea
author_facet Pinoni, Silvina Andrea
Lopez Mañanes, Alejandra Antonia
author_role author
author2 Lopez Mañanes, Alejandra Antonia
author2_role author
dc.subject.none.fl_str_mv CRABS
MUSCLE
NA+ ATPASES
NEOHELICE GRANULATA
OSMOIONOREGULATION
topic CRABS
MUSCLE
NA+ ATPASES
NEOHELICE GRANULATA
OSMOIONOREGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to environmental salinity of the coexistent Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities were studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+ ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemide-insensitive and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed Michaelis-Menten kinetics (Km = 0.021 and 0.224 mM, respectively). These characteristics appeared to be quite different from those previously described for Na+-K+ ATPase activity in chela muscle of this crab. Na+-K+ ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10‰), a salinity at which N. granulata exhibits a strong hyperregulatory capacity, Na+-K+ ATPase activity was higher (117 ± 26 nmol Pi min- 1 mg prot- 1) than in 35‰ salinity (23 ± 6 nmol Pi min- 1 mg prot- 1) (a salinity at which this crab is osmoionoconforming). On the contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35‰ salinity (108 ±15 nmol Pi min- 1 mg prot- 1) than in crabs acclimated to 10‰ salinity (36 ± 11 nmol Pi min- 1 mg prot- 1). Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to low salinity. The response to low salinity suggests that Na+-K+ ATPase could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive, furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible differential functional roles of Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are discussed.
Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
description The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to environmental salinity of the coexistent Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities were studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+ ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemide-insensitive and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed Michaelis-Menten kinetics (Km = 0.021 and 0.224 mM, respectively). These characteristics appeared to be quite different from those previously described for Na+-K+ ATPase activity in chela muscle of this crab. Na+-K+ ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10‰), a salinity at which N. granulata exhibits a strong hyperregulatory capacity, Na+-K+ ATPase activity was higher (117 ± 26 nmol Pi min- 1 mg prot- 1) than in 35‰ salinity (23 ± 6 nmol Pi min- 1 mg prot- 1) (a salinity at which this crab is osmoionoconforming). On the contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35‰ salinity (108 ±15 nmol Pi min- 1 mg prot- 1) than in crabs acclimated to 10‰ salinity (36 ± 11 nmol Pi min- 1 mg prot- 1). Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to low salinity. The response to low salinity suggests that Na+-K+ ATPase could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive, furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible differential functional roles of Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are discussed.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160062
Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 372; 1-2; 4-2009; 91-97
0022-0981
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160062
identifier_str_mv Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 372; 1-2; 4-2009; 91-97
0022-0981
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0022098109000914
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2009.02.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269173128888320
score 13.13397