Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro

Autores
Abrahan, Carolina Elizabeth; Insua, María Fernanda; Politi, Luis Enrique; German, Olga Lorena; Rotstein, Nora Patricia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxidative damage is involved in triggering neuronal death in several retinal neurodegenerative diseases. the recent finding of stem cells in the retina suggests that both preventing neuronal death and replacing lost neurons might be useful strategies for treating these diseases. we have previously shown that oxidative stress induces apoptosis in cultured retinal neurons. we now investigated the response of müller cells, proposed as retina stem cells, to this damage. treatment of glial cell cultures prepared from rat retinas with the oxidant paraquat (pq) did not induce glial cell apoptosis. instead, pq promoted their rapid dedifferentiation and proliferation. pq decreased expression of a marker of differentiated glial cells, simultaneously increasing the expression of smooth muscle actin, shown to increase with glial dedifferentiation, the levels of cell‐cycle markers, and the number of glial cells in the cultures. in addition, glial cells protected neurons in coculture from apoptosis induced by pq and h2o2. in pure neuronal cultures, pq induced apoptosis of photoreceptors and amacrine neurons, simultaneously decreasing the percentage of neurons preserving mitochondrial membrane potential; coculturing neurons with glial cells completely prevented pq‐induced apoptosis and preserved mitochondrial potential in both neuronal types. these results demonstrate that oxidative damage activated different responses in müller glial cells; they rapidly dedifferentiated and enhanced their proliferation, concurrently preventing neuronal apoptosis. glial cells might not only preserve neuronal survival but also activate their cell cycle in order to provide a pool of new progenitor cells that might eventually be manipulated to preserve retinal functionality.
Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Insua, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
Apoptosis
Cell Survival
Photoreceptor
Proliferation
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41485
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41485
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitroAbrahan, Carolina ElizabethInsua, María FernandaPoliti, Luis EnriqueGerman, Olga LorenaRotstein, Nora PatriciaApoptosisCell SurvivalPhotoreceptorProliferationOxidative Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oxidative damage is involved in triggering neuronal death in several retinal neurodegenerative diseases. the recent finding of stem cells in the retina suggests that both preventing neuronal death and replacing lost neurons might be useful strategies for treating these diseases. we have previously shown that oxidative stress induces apoptosis in cultured retinal neurons. we now investigated the response of müller cells, proposed as retina stem cells, to this damage. treatment of glial cell cultures prepared from rat retinas with the oxidant paraquat (pq) did not induce glial cell apoptosis. instead, pq promoted their rapid dedifferentiation and proliferation. pq decreased expression of a marker of differentiated glial cells, simultaneously increasing the expression of smooth muscle actin, shown to increase with glial dedifferentiation, the levels of cell‐cycle markers, and the number of glial cells in the cultures. in addition, glial cells protected neurons in coculture from apoptosis induced by pq and h2o2. in pure neuronal cultures, pq induced apoptosis of photoreceptors and amacrine neurons, simultaneously decreasing the percentage of neurons preserving mitochondrial membrane potential; coculturing neurons with glial cells completely prevented pq‐induced apoptosis and preserved mitochondrial potential in both neuronal types. these results demonstrate that oxidative damage activated different responses in müller glial cells; they rapidly dedifferentiated and enhanced their proliferation, concurrently preventing neuronal apoptosis. glial cells might not only preserve neuronal survival but also activate their cell cycle in order to provide a pool of new progenitor cells that might eventually be manipulated to preserve retinal functionality.Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Insua, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaWiley-liss, Div John Wiley & Sons Inc2009-03-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41485Abrahan, Carolina Elizabeth; Insua, María Fernanda; Politi, Luis Enrique; German, Olga Lorena; Rotstein, Nora Patricia; Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 87; 4; 14-3-2009; 964-9770360-4012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jnr.21903info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.21903info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:15Zoai:ri.conicet.gov.ar:11336/41485instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:16.086CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
title Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
spellingShingle Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
Abrahan, Carolina Elizabeth
Apoptosis
Cell Survival
Photoreceptor
Proliferation
Oxidative Stress
title_short Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
title_full Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
title_fullStr Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
title_full_unstemmed Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
title_sort Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro
dc.creator.none.fl_str_mv Abrahan, Carolina Elizabeth
Insua, María Fernanda
Politi, Luis Enrique
German, Olga Lorena
Rotstein, Nora Patricia
author Abrahan, Carolina Elizabeth
author_facet Abrahan, Carolina Elizabeth
Insua, María Fernanda
Politi, Luis Enrique
German, Olga Lorena
Rotstein, Nora Patricia
author_role author
author2 Insua, María Fernanda
Politi, Luis Enrique
German, Olga Lorena
Rotstein, Nora Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Cell Survival
Photoreceptor
Proliferation
Oxidative Stress
topic Apoptosis
Cell Survival
Photoreceptor
Proliferation
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxidative damage is involved in triggering neuronal death in several retinal neurodegenerative diseases. the recent finding of stem cells in the retina suggests that both preventing neuronal death and replacing lost neurons might be useful strategies for treating these diseases. we have previously shown that oxidative stress induces apoptosis in cultured retinal neurons. we now investigated the response of müller cells, proposed as retina stem cells, to this damage. treatment of glial cell cultures prepared from rat retinas with the oxidant paraquat (pq) did not induce glial cell apoptosis. instead, pq promoted their rapid dedifferentiation and proliferation. pq decreased expression of a marker of differentiated glial cells, simultaneously increasing the expression of smooth muscle actin, shown to increase with glial dedifferentiation, the levels of cell‐cycle markers, and the number of glial cells in the cultures. in addition, glial cells protected neurons in coculture from apoptosis induced by pq and h2o2. in pure neuronal cultures, pq induced apoptosis of photoreceptors and amacrine neurons, simultaneously decreasing the percentage of neurons preserving mitochondrial membrane potential; coculturing neurons with glial cells completely prevented pq‐induced apoptosis and preserved mitochondrial potential in both neuronal types. these results demonstrate that oxidative damage activated different responses in müller glial cells; they rapidly dedifferentiated and enhanced their proliferation, concurrently preventing neuronal apoptosis. glial cells might not only preserve neuronal survival but also activate their cell cycle in order to provide a pool of new progenitor cells that might eventually be manipulated to preserve retinal functionality.
Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Insua, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Oxidative damage is involved in triggering neuronal death in several retinal neurodegenerative diseases. the recent finding of stem cells in the retina suggests that both preventing neuronal death and replacing lost neurons might be useful strategies for treating these diseases. we have previously shown that oxidative stress induces apoptosis in cultured retinal neurons. we now investigated the response of müller cells, proposed as retina stem cells, to this damage. treatment of glial cell cultures prepared from rat retinas with the oxidant paraquat (pq) did not induce glial cell apoptosis. instead, pq promoted their rapid dedifferentiation and proliferation. pq decreased expression of a marker of differentiated glial cells, simultaneously increasing the expression of smooth muscle actin, shown to increase with glial dedifferentiation, the levels of cell‐cycle markers, and the number of glial cells in the cultures. in addition, glial cells protected neurons in coculture from apoptosis induced by pq and h2o2. in pure neuronal cultures, pq induced apoptosis of photoreceptors and amacrine neurons, simultaneously decreasing the percentage of neurons preserving mitochondrial membrane potential; coculturing neurons with glial cells completely prevented pq‐induced apoptosis and preserved mitochondrial potential in both neuronal types. these results demonstrate that oxidative damage activated different responses in müller glial cells; they rapidly dedifferentiated and enhanced their proliferation, concurrently preventing neuronal apoptosis. glial cells might not only preserve neuronal survival but also activate their cell cycle in order to provide a pool of new progenitor cells that might eventually be manipulated to preserve retinal functionality.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41485
Abrahan, Carolina Elizabeth; Insua, María Fernanda; Politi, Luis Enrique; German, Olga Lorena; Rotstein, Nora Patricia; Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 87; 4; 14-3-2009; 964-977
0360-4012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41485
identifier_str_mv Abrahan, Carolina Elizabeth; Insua, María Fernanda; Politi, Luis Enrique; German, Olga Lorena; Rotstein, Nora Patricia; Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro; Wiley-liss, Div John Wiley & Sons Inc; Journal of Neuroscience Research; 87; 4; 14-3-2009; 964-977
0360-4012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jnr.21903
info:eu-repo/semantics/altIdentifier/doi/10.1002/jnr.21903
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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