Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats

Autores
Cots, Debora Soledad; Rolando, Alicia Nelida; Mugnaini, Maria Teresa; Soñez, Carlos Alberto; Bozzo, Aida Andrea; Borghi, Damiana; Gauana, Hector Fernando; Romanini, María Cristina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic stress during gestation can alter several mechanisms that maintain homeostasis in the placenta. The aim of this study was to determine both effects of chronic stress on the apoptotic and cell proliferation processes in placentas of rats, during the second half of pregnancy and the immunolocalization of apoptotic and proliferating marked nuclei in the different placental zones. Stress by immobilization was applied to rats from the 4th day of pregnancy until their sacrifice on days 12, 17 or 21. Placental sections were immunolabeled with anti-BrdU and TUNEL. The apoptotic index did not present significant differences between control and stress groups. The gestational day influenced significantly on the apoptotic index, which showed a significant decrease on day 17 of gestation and significant increase on day 21. The proliferating index presented significant differences between gestational stages studied, which showed a significant increase at day 17 and then a significant decrease at day 21. Moreover, the stress treatment applied highly influenced on the proliferation index. In conclusion, chronic stress by immobilization did not produce effects on apoptotic process. However it produces an increase in cellular proliferation index in the three gestation days analyzed, which could generate a deleterious environment for the fetus development
Fil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Mugnaini, Maria Teresa. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Borghi, Damiana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Gauana, Hector Fernando. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Materia
CHRONIC STRESS
APOPTOSIS
CELL PROLIFERATION
PLACENTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180724
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180724
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed RatsCots, Debora SoledadRolando, Alicia NelidaMugnaini, Maria TeresaSoñez, Carlos AlbertoBozzo, Aida AndreaBorghi, DamianaGauana, Hector FernandoRomanini, María CristinaCHRONIC STRESSAPOPTOSISCELL PROLIFERATIONPLACENTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chronic stress during gestation can alter several mechanisms that maintain homeostasis in the placenta. The aim of this study was to determine both effects of chronic stress on the apoptotic and cell proliferation processes in placentas of rats, during the second half of pregnancy and the immunolocalization of apoptotic and proliferating marked nuclei in the different placental zones. Stress by immobilization was applied to rats from the 4th day of pregnancy until their sacrifice on days 12, 17 or 21. Placental sections were immunolabeled with anti-BrdU and TUNEL. The apoptotic index did not present significant differences between control and stress groups. The gestational day influenced significantly on the apoptotic index, which showed a significant decrease on day 17 of gestation and significant increase on day 21. The proliferating index presented significant differences between gestational stages studied, which showed a significant increase at day 17 and then a significant decrease at day 21. Moreover, the stress treatment applied highly influenced on the proliferation index. In conclusion, chronic stress by immobilization did not produce effects on apoptotic process. However it produces an increase in cellular proliferation index in the three gestation days analyzed, which could generate a deleterious environment for the fetus developmentFil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaFil: Mugnaini, Maria Teresa. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaFil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaFil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaFil: Borghi, Damiana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Gauana, Hector Fernando. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaFil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; ArgentinaOpen Access Scientific Reports2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180724Cots, Debora Soledad; Rolando, Alicia Nelida; Mugnaini, Maria Teresa; Soñez, Carlos Alberto; Bozzo, Aida Andrea; et al.; Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats; Open Access Scientific Reports; Journal of Cytology and Histology; 2; 3; 3-2013; 1-52157-7099CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/scientificreports.664info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/scientific-reports/srep664.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:06Zoai:ri.conicet.gov.ar:11336/180724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:07.129CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
title Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
spellingShingle Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
Cots, Debora Soledad
CHRONIC STRESS
APOPTOSIS
CELL PROLIFERATION
PLACENTA
title_short Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
title_full Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
title_fullStr Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
title_full_unstemmed Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
title_sort Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats
dc.creator.none.fl_str_mv Cots, Debora Soledad
Rolando, Alicia Nelida
Mugnaini, Maria Teresa
Soñez, Carlos Alberto
Bozzo, Aida Andrea
Borghi, Damiana
Gauana, Hector Fernando
Romanini, María Cristina
author Cots, Debora Soledad
author_facet Cots, Debora Soledad
Rolando, Alicia Nelida
Mugnaini, Maria Teresa
Soñez, Carlos Alberto
Bozzo, Aida Andrea
Borghi, Damiana
Gauana, Hector Fernando
Romanini, María Cristina
author_role author
author2 Rolando, Alicia Nelida
Mugnaini, Maria Teresa
Soñez, Carlos Alberto
Bozzo, Aida Andrea
Borghi, Damiana
Gauana, Hector Fernando
Romanini, María Cristina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHRONIC STRESS
APOPTOSIS
CELL PROLIFERATION
PLACENTA
topic CHRONIC STRESS
APOPTOSIS
CELL PROLIFERATION
PLACENTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chronic stress during gestation can alter several mechanisms that maintain homeostasis in the placenta. The aim of this study was to determine both effects of chronic stress on the apoptotic and cell proliferation processes in placentas of rats, during the second half of pregnancy and the immunolocalization of apoptotic and proliferating marked nuclei in the different placental zones. Stress by immobilization was applied to rats from the 4th day of pregnancy until their sacrifice on days 12, 17 or 21. Placental sections were immunolabeled with anti-BrdU and TUNEL. The apoptotic index did not present significant differences between control and stress groups. The gestational day influenced significantly on the apoptotic index, which showed a significant decrease on day 17 of gestation and significant increase on day 21. The proliferating index presented significant differences between gestational stages studied, which showed a significant increase at day 17 and then a significant decrease at day 21. Moreover, the stress treatment applied highly influenced on the proliferation index. In conclusion, chronic stress by immobilization did not produce effects on apoptotic process. However it produces an increase in cellular proliferation index in the three gestation days analyzed, which could generate a deleterious environment for the fetus development
Fil: Cots, Debora Soledad. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rolando, Alicia Nelida. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Mugnaini, Maria Teresa. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Soñez, Carlos Alberto. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Bozzo, Aida Andrea. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Borghi, Damiana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Gauana, Hector Fernando. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
Fil: Romanini, María Cristina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal. Laboratorio de Biología Cecular y Embriologia; Argentina
description Chronic stress during gestation can alter several mechanisms that maintain homeostasis in the placenta. The aim of this study was to determine both effects of chronic stress on the apoptotic and cell proliferation processes in placentas of rats, during the second half of pregnancy and the immunolocalization of apoptotic and proliferating marked nuclei in the different placental zones. Stress by immobilization was applied to rats from the 4th day of pregnancy until their sacrifice on days 12, 17 or 21. Placental sections were immunolabeled with anti-BrdU and TUNEL. The apoptotic index did not present significant differences between control and stress groups. The gestational day influenced significantly on the apoptotic index, which showed a significant decrease on day 17 of gestation and significant increase on day 21. The proliferating index presented significant differences between gestational stages studied, which showed a significant increase at day 17 and then a significant decrease at day 21. Moreover, the stress treatment applied highly influenced on the proliferation index. In conclusion, chronic stress by immobilization did not produce effects on apoptotic process. However it produces an increase in cellular proliferation index in the three gestation days analyzed, which could generate a deleterious environment for the fetus development
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180724
Cots, Debora Soledad; Rolando, Alicia Nelida; Mugnaini, Maria Teresa; Soñez, Carlos Alberto; Bozzo, Aida Andrea; et al.; Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats; Open Access Scientific Reports; Journal of Cytology and Histology; 2; 3; 3-2013; 1-5
2157-7099
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180724
identifier_str_mv Cots, Debora Soledad; Rolando, Alicia Nelida; Mugnaini, Maria Teresa; Soñez, Carlos Alberto; Bozzo, Aida Andrea; et al.; Determination of Cell Proliferation and Apoptosis in Placentas of Chronically Stressed Rats; Open Access Scientific Reports; Journal of Cytology and Histology; 2; 3; 3-2013; 1-5
2157-7099
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/scientificreports.664
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/scientific-reports/srep664.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Access Scientific Reports
publisher.none.fl_str_mv Open Access Scientific Reports
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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