Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid

Autores
Chucair, Ana Julia; Rotstein, Nora Patricia; Sangiovanni, John Paul; During, Alexandrine; Chew, Emily Y.; Politi, Luis Enrique
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: Oxidative stress has been proposed as a major pathogenic factor in age-related macular degeneration (AMD), the leading cause of vision loss among elderly people of western European ancestry. Lutein (LUT) and zeaxanthin (ZEA), major components in macular pigment, are among the retinal antioxidants. Though xanthophyll intake may reduce the likelihood of having advanced AMD, direct evidence of neuroprotection is lacking. Prior work has shown that docosahexaenoic acid (DHA), the major polyunsaturated fatty acid in the retina, delays apoptosis and promotes differentiation of photoreceptors. This study was conducted to investigate whether LUT, ZEA, and β-carotene (BC), major dietary carotenoids protect photoreceptors from oxidative stress and whether this protection is synergistic with that of DHA. Methods: Pure rat retinal neurons in culture, supplemented with LUT, ZEA, or BC, with or without DHA, were subjected to oxidative stress induced with paraquat and hydrogen peroxide. Apoptosis, preservation of mitochondrial membrane potential, cytochrome c translocation, and opsin expression were evaluated. Results: Pretreatment with DHA, LUT, ZEA, and BC reduced oxidative stress-induced apoptosis in photoreceptors, preserved mitochondrial potential, and prevented cytochrome c release from mitochondria. ZEA and LUT also enhanced photoreceptor differentiation. In control cultures, photoreceptors failed to grow their characteristic outer segments; addition of DHA, ZEA, or LUT increased opsin expression and promoted the development of outer-segment–like processes. Conclusions: These results show for the first time the direct neuroprotection of photoreceptors by xanthophylls and suggest that ZEA and LUT, along with DHA, are important environmental influences that together promote photoreceptor survival and differentiation.
Fil: Chucair, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sangiovanni, John Paul. National Institutes of Health; Estados Unidos
Fil: During, Alexandrine. Université Catholique de Louvain; Bélgica
Fil: Chew, Emily Y.. National Institutes of Health; Estados Unidos
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
Carotenoid
Photoreceptor
Oxidative Stress
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44699
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44699
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic AcidChucair, Ana JuliaRotstein, Nora PatriciaSangiovanni, John PaulDuring, AlexandrineChew, Emily Y.Politi, Luis EnriqueCarotenoidPhotoreceptorOxidative StressApoptosishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Purpose: Oxidative stress has been proposed as a major pathogenic factor in age-related macular degeneration (AMD), the leading cause of vision loss among elderly people of western European ancestry. Lutein (LUT) and zeaxanthin (ZEA), major components in macular pigment, are among the retinal antioxidants. Though xanthophyll intake may reduce the likelihood of having advanced AMD, direct evidence of neuroprotection is lacking. Prior work has shown that docosahexaenoic acid (DHA), the major polyunsaturated fatty acid in the retina, delays apoptosis and promotes differentiation of photoreceptors. This study was conducted to investigate whether LUT, ZEA, and β-carotene (BC), major dietary carotenoids protect photoreceptors from oxidative stress and whether this protection is synergistic with that of DHA. Methods: Pure rat retinal neurons in culture, supplemented with LUT, ZEA, or BC, with or without DHA, were subjected to oxidative stress induced with paraquat and hydrogen peroxide. Apoptosis, preservation of mitochondrial membrane potential, cytochrome c translocation, and opsin expression were evaluated. Results: Pretreatment with DHA, LUT, ZEA, and BC reduced oxidative stress-induced apoptosis in photoreceptors, preserved mitochondrial potential, and prevented cytochrome c release from mitochondria. ZEA and LUT also enhanced photoreceptor differentiation. In control cultures, photoreceptors failed to grow their characteristic outer segments; addition of DHA, ZEA, or LUT increased opsin expression and promoted the development of outer-segment–like processes. Conclusions: These results show for the first time the direct neuroprotection of photoreceptors by xanthophylls and suggest that ZEA and LUT, along with DHA, are important environmental influences that together promote photoreceptor survival and differentiation.Fil: Chucair, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Sangiovanni, John Paul. National Institutes of Health; Estados UnidosFil: During, Alexandrine. Université Catholique de Louvain; BélgicaFil: Chew, Emily Y.. National Institutes of Health; Estados UnidosFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAssociation for Research in Vision and Ophthalmology2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44699Chucair, Ana Julia; Rotstein, Nora Patricia; Sangiovanni, John Paul; During, Alexandrine; Chew, Emily Y.; et al.; Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 48; 11; 11-2007; 5168-51770146-04041552-5783CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2183112info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.07-0037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:44Zoai:ri.conicet.gov.ar:11336/44699instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:44.798CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
title Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
spellingShingle Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
Chucair, Ana Julia
Carotenoid
Photoreceptor
Oxidative Stress
Apoptosis
title_short Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
title_full Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
title_fullStr Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
title_full_unstemmed Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
title_sort Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
dc.creator.none.fl_str_mv Chucair, Ana Julia
Rotstein, Nora Patricia
Sangiovanni, John Paul
During, Alexandrine
Chew, Emily Y.
Politi, Luis Enrique
author Chucair, Ana Julia
author_facet Chucair, Ana Julia
Rotstein, Nora Patricia
Sangiovanni, John Paul
During, Alexandrine
Chew, Emily Y.
Politi, Luis Enrique
author_role author
author2 Rotstein, Nora Patricia
Sangiovanni, John Paul
During, Alexandrine
Chew, Emily Y.
Politi, Luis Enrique
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Carotenoid
Photoreceptor
Oxidative Stress
Apoptosis
topic Carotenoid
Photoreceptor
Oxidative Stress
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Purpose: Oxidative stress has been proposed as a major pathogenic factor in age-related macular degeneration (AMD), the leading cause of vision loss among elderly people of western European ancestry. Lutein (LUT) and zeaxanthin (ZEA), major components in macular pigment, are among the retinal antioxidants. Though xanthophyll intake may reduce the likelihood of having advanced AMD, direct evidence of neuroprotection is lacking. Prior work has shown that docosahexaenoic acid (DHA), the major polyunsaturated fatty acid in the retina, delays apoptosis and promotes differentiation of photoreceptors. This study was conducted to investigate whether LUT, ZEA, and β-carotene (BC), major dietary carotenoids protect photoreceptors from oxidative stress and whether this protection is synergistic with that of DHA. Methods: Pure rat retinal neurons in culture, supplemented with LUT, ZEA, or BC, with or without DHA, were subjected to oxidative stress induced with paraquat and hydrogen peroxide. Apoptosis, preservation of mitochondrial membrane potential, cytochrome c translocation, and opsin expression were evaluated. Results: Pretreatment with DHA, LUT, ZEA, and BC reduced oxidative stress-induced apoptosis in photoreceptors, preserved mitochondrial potential, and prevented cytochrome c release from mitochondria. ZEA and LUT also enhanced photoreceptor differentiation. In control cultures, photoreceptors failed to grow their characteristic outer segments; addition of DHA, ZEA, or LUT increased opsin expression and promoted the development of outer-segment–like processes. Conclusions: These results show for the first time the direct neuroprotection of photoreceptors by xanthophylls and suggest that ZEA and LUT, along with DHA, are important environmental influences that together promote photoreceptor survival and differentiation.
Fil: Chucair, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sangiovanni, John Paul. National Institutes of Health; Estados Unidos
Fil: During, Alexandrine. Université Catholique de Louvain; Bélgica
Fil: Chew, Emily Y.. National Institutes of Health; Estados Unidos
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Purpose: Oxidative stress has been proposed as a major pathogenic factor in age-related macular degeneration (AMD), the leading cause of vision loss among elderly people of western European ancestry. Lutein (LUT) and zeaxanthin (ZEA), major components in macular pigment, are among the retinal antioxidants. Though xanthophyll intake may reduce the likelihood of having advanced AMD, direct evidence of neuroprotection is lacking. Prior work has shown that docosahexaenoic acid (DHA), the major polyunsaturated fatty acid in the retina, delays apoptosis and promotes differentiation of photoreceptors. This study was conducted to investigate whether LUT, ZEA, and β-carotene (BC), major dietary carotenoids protect photoreceptors from oxidative stress and whether this protection is synergistic with that of DHA. Methods: Pure rat retinal neurons in culture, supplemented with LUT, ZEA, or BC, with or without DHA, were subjected to oxidative stress induced with paraquat and hydrogen peroxide. Apoptosis, preservation of mitochondrial membrane potential, cytochrome c translocation, and opsin expression were evaluated. Results: Pretreatment with DHA, LUT, ZEA, and BC reduced oxidative stress-induced apoptosis in photoreceptors, preserved mitochondrial potential, and prevented cytochrome c release from mitochondria. ZEA and LUT also enhanced photoreceptor differentiation. In control cultures, photoreceptors failed to grow their characteristic outer segments; addition of DHA, ZEA, or LUT increased opsin expression and promoted the development of outer-segment–like processes. Conclusions: These results show for the first time the direct neuroprotection of photoreceptors by xanthophylls and suggest that ZEA and LUT, along with DHA, are important environmental influences that together promote photoreceptor survival and differentiation.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44699
Chucair, Ana Julia; Rotstein, Nora Patricia; Sangiovanni, John Paul; During, Alexandrine; Chew, Emily Y.; et al.; Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 48; 11; 11-2007; 5168-5177
0146-0404
1552-5783
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44699
identifier_str_mv Chucair, Ana Julia; Rotstein, Nora Patricia; Sangiovanni, John Paul; During, Alexandrine; Chew, Emily Y.; et al.; Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 48; 11; 11-2007; 5168-5177
0146-0404
1552-5783
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2183112
info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.07-0037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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