Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor

Autores
German, Olga Lorena; Monaco, Sandra Tanya; Agnolazza, Daniela Luciana; Rotstein, Nora Patricia; Politi, Luis Enrique
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Docosahexaenoic acid (DHA), the major omega-3 polyunsaturated fatty acid in the retina, promotes the survival of rat retina photoreceptors (PR) during early development in vitro and upon oxidative stress by activating the ERK/MAPK signaling pathway. We investigated if DHA activates this pathway by direct activation of tyrosine kinase receptors (TRK) or of retinoid nuclear receptors (RXR). Using retinal neuronal cultures we determined that DHA prevented PR apoptosis at early culture times in spite of the presence of a TRK inhibitor (K252a), implying TRK are not involved in its effects. On the contrary, RXR antagonists (HX531 or PA452) inhibited DHA protection during early development in vitro and upon paraquat and H2O2-induced apoptosis. Moreover, RXR agonists (HX630 or PA024) decreased photoreceptors apoptosis in H2O2-treated neuronal cultures, as we previously showed for DHA. To evaluate whether DHA has to be released from phospholipids to exert its protective effect, DHA-supplemented cultures were treated with a phospholipase A2 inhibitor (BEL) prior to H2O2 treatment; BEL addition blocked DHA protection on PR upon oxidative stress. These results suggest a new pathway for DHA actions in PR: it is first released from phospholipids and then activates RXR to promote PR survival.
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Monaco, Sandra Tanya. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia Molecular
Foz do Iguacu
Brasil
Sociedade Brasileira de Bioquimica e Biologia Molecular
Materia
PHOTORECEPTOR
DHA
APOPTOSIS
RXR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253064
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/253064
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptorGerman, Olga LorenaMonaco, Sandra TanyaAgnolazza, Daniela LucianaRotstein, Nora PatriciaPoliti, Luis EnriquePHOTORECEPTORDHAAPOPTOSISRXRhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Docosahexaenoic acid (DHA), the major omega-3 polyunsaturated fatty acid in the retina, promotes the survival of rat retina photoreceptors (PR) during early development in vitro and upon oxidative stress by activating the ERK/MAPK signaling pathway. We investigated if DHA activates this pathway by direct activation of tyrosine kinase receptors (TRK) or of retinoid nuclear receptors (RXR). Using retinal neuronal cultures we determined that DHA prevented PR apoptosis at early culture times in spite of the presence of a TRK inhibitor (K252a), implying TRK are not involved in its effects. On the contrary, RXR antagonists (HX531 or PA452) inhibited DHA protection during early development in vitro and upon paraquat and H2O2-induced apoptosis. Moreover, RXR agonists (HX630 or PA024) decreased photoreceptors apoptosis in H2O2-treated neuronal cultures, as we previously showed for DHA. To evaluate whether DHA has to be released from phospholipids to exert its protective effect, DHA-supplemented cultures were treated with a phospholipase A2 inhibitor (BEL) prior to H2O2 treatment; BEL addition blocked DHA protection on PR upon oxidative stress. These results suggest a new pathway for DHA actions in PR: it is first released from phospholipids and then activates RXR to promote PR survival.Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Monaco, Sandra Tanya. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia MolecularFoz do IguacuBrasilSociedade Brasileira de Bioquimica e Biologia MolecularSociedade Brasileira de Bioquimica e Biologia Molecular2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253064Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor; XLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia Molecular; Foz do Iguacu; Brasil; 2012; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www2.sbbq.org.br/reuniao/reuniao2012.htmlinfo:eu-repo/semantics/altIdentifier/url/https://www2.sbbq.org.br/reuniao/cdrom/ra2012/abstract.htmInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:41Zoai:ri.conicet.gov.ar:11336/253064instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:41.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
title Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
spellingShingle Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
German, Olga Lorena
PHOTORECEPTOR
DHA
APOPTOSIS
RXR
title_short Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
title_full Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
title_fullStr Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
title_full_unstemmed Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
title_sort Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor
dc.creator.none.fl_str_mv German, Olga Lorena
Monaco, Sandra Tanya
Agnolazza, Daniela Luciana
Rotstein, Nora Patricia
Politi, Luis Enrique
author German, Olga Lorena
author_facet German, Olga Lorena
Monaco, Sandra Tanya
Agnolazza, Daniela Luciana
Rotstein, Nora Patricia
Politi, Luis Enrique
author_role author
author2 Monaco, Sandra Tanya
Agnolazza, Daniela Luciana
Rotstein, Nora Patricia
Politi, Luis Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHOTORECEPTOR
DHA
APOPTOSIS
RXR
topic PHOTORECEPTOR
DHA
APOPTOSIS
RXR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Docosahexaenoic acid (DHA), the major omega-3 polyunsaturated fatty acid in the retina, promotes the survival of rat retina photoreceptors (PR) during early development in vitro and upon oxidative stress by activating the ERK/MAPK signaling pathway. We investigated if DHA activates this pathway by direct activation of tyrosine kinase receptors (TRK) or of retinoid nuclear receptors (RXR). Using retinal neuronal cultures we determined that DHA prevented PR apoptosis at early culture times in spite of the presence of a TRK inhibitor (K252a), implying TRK are not involved in its effects. On the contrary, RXR antagonists (HX531 or PA452) inhibited DHA protection during early development in vitro and upon paraquat and H2O2-induced apoptosis. Moreover, RXR agonists (HX630 or PA024) decreased photoreceptors apoptosis in H2O2-treated neuronal cultures, as we previously showed for DHA. To evaluate whether DHA has to be released from phospholipids to exert its protective effect, DHA-supplemented cultures were treated with a phospholipase A2 inhibitor (BEL) prior to H2O2 treatment; BEL addition blocked DHA protection on PR upon oxidative stress. These results suggest a new pathway for DHA actions in PR: it is first released from phospholipids and then activates RXR to promote PR survival.
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Monaco, Sandra Tanya. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia Molecular
Foz do Iguacu
Brasil
Sociedade Brasileira de Bioquimica e Biologia Molecular
description Docosahexaenoic acid (DHA), the major omega-3 polyunsaturated fatty acid in the retina, promotes the survival of rat retina photoreceptors (PR) during early development in vitro and upon oxidative stress by activating the ERK/MAPK signaling pathway. We investigated if DHA activates this pathway by direct activation of tyrosine kinase receptors (TRK) or of retinoid nuclear receptors (RXR). Using retinal neuronal cultures we determined that DHA prevented PR apoptosis at early culture times in spite of the presence of a TRK inhibitor (K252a), implying TRK are not involved in its effects. On the contrary, RXR antagonists (HX531 or PA452) inhibited DHA protection during early development in vitro and upon paraquat and H2O2-induced apoptosis. Moreover, RXR agonists (HX630 or PA024) decreased photoreceptors apoptosis in H2O2-treated neuronal cultures, as we previously showed for DHA. To evaluate whether DHA has to be released from phospholipids to exert its protective effect, DHA-supplemented cultures were treated with a phospholipase A2 inhibitor (BEL) prior to H2O2 treatment; BEL addition blocked DHA protection on PR upon oxidative stress. These results suggest a new pathway for DHA actions in PR: it is first released from phospholipids and then activates RXR to promote PR survival.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253064
Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor; XLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia Molecular; Foz do Iguacu; Brasil; 2012; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253064
identifier_str_mv Unesterified docosahexaenoic acid promotes photoreceptors survival activating a retinoid orphan receptor; XLI Reuniao Anual da Sociedade Brasileira de Bioquimica e Biologia Molecular; Foz do Iguacu; Brasil; 2012; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://www2.sbbq.org.br/reuniao/cdrom/ra2012/abstract.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedade Brasileira de Bioquimica e Biologia Molecular
publisher.none.fl_str_mv Sociedade Brasileira de Bioquimica e Biologia Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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