Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury

Autores
Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; Montaner, Alejandro Daniel; Villar, Marcelo Jose
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina
Fil: Hernando Insúa, Andrés. Immunotech S.a.; Argentina
Fil: Rodriguez, Juan Manuel. Immunotech S.a.; Argentina
Fil: Elias, Fernanda. Immunotech S.a.; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Montaner, Alejandro Daniel. Immunotech S.a.; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina
Materia
Pain
Hyperalgesia
Oligodeoxyribonucleotides
Sciatic Neurophaty
Stromal Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25981
Acceder
id CONICETDig_dc9bfee3f8b80f138db32f9842a0fec9
oai_identifier_str oai:ri.conicet.gov.ar:11336/25981
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injuryCoronel, Maria FlorenciaHernando Insúa, AndrésRodriguez, Juan ManuelElias, FernandaChasseing, Norma AlejandraMontaner, Alejandro DanielVillar, Marcelo JosePainHyperalgesiaOligodeoxyribonucleotidesSciatic NeurophatyStromal Cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.Fil: Coronel, Maria Florencia. Universidad Austral; ArgentinaFil: Hernando Insúa, Andrés. Immunotech S.a.; ArgentinaFil: Rodriguez, Juan Manuel. Immunotech S.a.; ArgentinaFil: Elias, Fernanda. Immunotech S.a.; ArgentinaFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Montaner, Alejandro Daniel. Immunotech S.a.; ArgentinaFil: Villar, Marcelo Jose. Universidad Austral; ArgentinaElsevier Ireland2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25981Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-730304-39401872-7972CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394008010185info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2008.07.045info:eu-repo/semantics/altIdentifier/pmid/18672022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:59Zoai:ri.conicet.gov.ar:11336/25981instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:00.121CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
title Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
spellingShingle Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
Coronel, Maria Florencia
Pain
Hyperalgesia
Oligodeoxyribonucleotides
Sciatic Neurophaty
Stromal Cells
title_short Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
title_full Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
title_fullStr Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
title_full_unstemmed Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
title_sort Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
dc.creator.none.fl_str_mv Coronel, Maria Florencia
Hernando Insúa, Andrés
Rodriguez, Juan Manuel
Elias, Fernanda
Chasseing, Norma Alejandra
Montaner, Alejandro Daniel
Villar, Marcelo Jose
author Coronel, Maria Florencia
author_facet Coronel, Maria Florencia
Hernando Insúa, Andrés
Rodriguez, Juan Manuel
Elias, Fernanda
Chasseing, Norma Alejandra
Montaner, Alejandro Daniel
Villar, Marcelo Jose
author_role author
author2 Hernando Insúa, Andrés
Rodriguez, Juan Manuel
Elias, Fernanda
Chasseing, Norma Alejandra
Montaner, Alejandro Daniel
Villar, Marcelo Jose
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pain
Hyperalgesia
Oligodeoxyribonucleotides
Sciatic Neurophaty
Stromal Cells
topic Pain
Hyperalgesia
Oligodeoxyribonucleotides
Sciatic Neurophaty
Stromal Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina
Fil: Hernando Insúa, Andrés. Immunotech S.a.; Argentina
Fil: Rodriguez, Juan Manuel. Immunotech S.a.; Argentina
Fil: Elias, Fernanda. Immunotech S.a.; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Montaner, Alejandro Daniel. Immunotech S.a.; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina
description We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.
publishDate 2008
dc.date.none.fl_str_mv 2008-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25981
Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-73
0304-3940
1872-7972
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25981
identifier_str_mv Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-73
0304-3940
1872-7972
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394008010185
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2008.07.045
info:eu-repo/semantics/altIdentifier/pmid/18672022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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