Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury
- Autores
- Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; Montaner, Alejandro Daniel; Villar, Marcelo Jose
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina
Fil: Hernando Insúa, Andrés. Immunotech S.a.; Argentina
Fil: Rodriguez, Juan Manuel. Immunotech S.a.; Argentina
Fil: Elias, Fernanda. Immunotech S.a.; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Montaner, Alejandro Daniel. Immunotech S.a.; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina - Materia
-
Pain
Hyperalgesia
Oligodeoxyribonucleotides
Sciatic Neurophaty
Stromal Cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/25981
Ver los metadatos del registro completo
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Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injuryCoronel, Maria FlorenciaHernando Insúa, AndrésRodriguez, Juan ManuelElias, FernandaChasseing, Norma AlejandraMontaner, Alejandro DanielVillar, Marcelo JosePainHyperalgesiaOligodeoxyribonucleotidesSciatic NeurophatyStromal Cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain.Fil: Coronel, Maria Florencia. Universidad Austral; ArgentinaFil: Hernando Insúa, Andrés. Immunotech S.a.; ArgentinaFil: Rodriguez, Juan Manuel. Immunotech S.a.; ArgentinaFil: Elias, Fernanda. Immunotech S.a.; ArgentinaFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Montaner, Alejandro Daniel. Immunotech S.a.; ArgentinaFil: Villar, Marcelo Jose. Universidad Austral; ArgentinaElsevier Ireland2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25981Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-730304-39401872-7972CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394008010185info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2008.07.045info:eu-repo/semantics/altIdentifier/pmid/18672022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:17Zoai:ri.conicet.gov.ar:11336/25981instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:17.498CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
title |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
spellingShingle |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury Coronel, Maria Florencia Pain Hyperalgesia Oligodeoxyribonucleotides Sciatic Neurophaty Stromal Cells |
title_short |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
title_full |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
title_fullStr |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
title_full_unstemmed |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
title_sort |
Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury |
dc.creator.none.fl_str_mv |
Coronel, Maria Florencia Hernando Insúa, Andrés Rodriguez, Juan Manuel Elias, Fernanda Chasseing, Norma Alejandra Montaner, Alejandro Daniel Villar, Marcelo Jose |
author |
Coronel, Maria Florencia |
author_facet |
Coronel, Maria Florencia Hernando Insúa, Andrés Rodriguez, Juan Manuel Elias, Fernanda Chasseing, Norma Alejandra Montaner, Alejandro Daniel Villar, Marcelo Jose |
author_role |
author |
author2 |
Hernando Insúa, Andrés Rodriguez, Juan Manuel Elias, Fernanda Chasseing, Norma Alejandra Montaner, Alejandro Daniel Villar, Marcelo Jose |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Pain Hyperalgesia Oligodeoxyribonucleotides Sciatic Neurophaty Stromal Cells |
topic |
Pain Hyperalgesia Oligodeoxyribonucleotides Sciatic Neurophaty Stromal Cells |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain. Fil: Coronel, Maria Florencia. Universidad Austral; Argentina Fil: Hernando Insúa, Andrés. Immunotech S.a.; Argentina Fil: Rodriguez, Juan Manuel. Immunotech S.a.; Argentina Fil: Elias, Fernanda. Immunotech S.a.; Argentina Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Montaner, Alejandro Daniel. Immunotech S.a.; Argentina Fil: Villar, Marcelo Jose. Universidad Austral; Argentina |
description |
We have recently shown that the administration of bone marrow stromal cells (MSCs) prevents the development of mechanical and thermal allodynia in animals subjected to a sciatic nerve injury. Furthermore, exogenously administered MSCs have been shown to participate in the repair and regeneration of damaged tissues in a variety of animal models. However, some limitations of this therapeutic approach, basically related to the ex vivo cell manipulation procedure, have arisen. IMT504, the prototype of the PyNTTTTGT class of immunostimulatory oligonucleotides, stimulates MSC expansion both in vitro and in vivo. In this study, we evaluated the effect of IMT504 systemic administration on the development of mechanical and thermal allodynia in rats subjected to a sciatic nerve crush. Animals were treated with IMT504, MSCs or saline either immediately after performing the lesion or 4 days after it, and were evaluated using the von Frey and Choi tests at different times after injury. Control animals developed both mechanical and thermal allodynia. Animals receiving either IMT504 or MSCs immediately after injury did not develop mechanical allodynia and presented a significantly lower number of nociceptive responses to cold stimulation as compared to controls. Moreover, injury-induced allodynia was significantly reduced after IMT504 delayed treatment. Our results show that the administration of IMT504 reduces neuropathic pain-associated behaviors, suggesting that IMT504 could represent a possible therapeutic approach for the treatment of neuropathic pain. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/25981 Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-73 0304-3940 1872-7972 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/25981 |
identifier_str_mv |
Coronel, Maria Florencia; Hernando Insúa, Andrés; Rodriguez, Juan Manuel; Elias, Fernanda; Chasseing, Norma Alejandra; et al.; Oligonucleotide IMT504 reduces neuropathic pain after peripheral nerve injury; Elsevier Ireland; Neuroscience Letters; 444; 1; 10-2008; 69-73 0304-3940 1872-7972 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394008010185 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2008.07.045 info:eu-repo/semantics/altIdentifier/pmid/18672022 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Ireland |
publisher.none.fl_str_mv |
Elsevier Ireland |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |