Bone marrow stromal cells attenuate injury-induced changes in galanin, NPY and NPY Y1 receptor expression after a sciatic nerve constriction

• Autores: Coronel, Maria Florencia; Musolino, P. L.; Brumovsky, Pablo Rodolfo; Hökfelt, T.; Villar, M. J.
• Año de publicación: 2009
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Pain; Bone Marrow Cells; Neuropeptide Y; Sciatic Nerve; Stromal Cells
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Single ligature nerve constriction (SLNC) of the rat sciatic nerve triggers neuropathic pain-related behaviors and induces changes in neuropeptide expression in primary afferent neurons. Bone marrow stromal cells (MSCs) injected into the lumbar 4 (L4) dorsal root ganglia (DRGs) of animals subjected to a sciatic nerve SLNC selectively migrate to the other ipsilateral lumbar DRGs (L3, L5 and L6) and prevent mechanical and thermal allodynia. In this study, we have evaluated the effect of MSC administration on the expression of the neuropeptides galanin and NPY, as well as the NPY Y(1)-receptor (Y(1)R) in DRG neurons. Animals were subjected to a sciatic nerve SLNC either alone or followed by the administration of MSCs, phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs), directly into the ipsilateral L4 DRG. Seven days after injury, the ipsilateral and contralateral L4-5 DRGs were dissected out and processed for standard immunohistochemistry, using specific antibodies. As previously reported, SLNC induced an ipsilateral increase in the number of galanin and NPY immunoreactive neurons and a decrease in Y(1)R-positive DRG neurons. The intraganglionic injection of PBS or BNMCs did not modify this pattern of expression. In contrast, MSC administration partially prevented the injury-induced changes in galanin, NPY and Y(1)R expression. The large number of Y(1)R-immunoreactive neurons together with high levels of NPY expression in animals injected with MSCs could explain, at least in part, the analgesic effects exerted by these cells. Our results support MSC participation in the modulation of neuropathic pain and give insight into one of the possible mechanisms involved.
Fil: Coronel, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Austral; Argentina
Fil: Musolino, P. L.. Universidad Austral; Argentina
Fil: Brumovsky, Pablo Rodolfo. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Hökfelt, T.. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Villar, M. J.. Universidad Austral; Argentina