Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction

Autores
Musolino, Patricia Leonor; Coronel, Maria Florencia; Hökfelt, Tomas; Villar, Marcelo José
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peripheral nerve injury, i.e. a single ligature nerve constriction (SLNC), triggers neuropathic pain. Bone marrow stromal cells (MSCs) have been observed to migrate to the injured tissues and mediate functional recovery following brain, spinal cord and peripheral nerve lesions. We have recently shown MSC selective migration to the ipsilateral lumbar (L3-6) dorsal root ganglia (DRGs) after a sciatic nerve SLNC. In this study, we have analyzed the thermal and mechanical sensitivities of animals subjected to a SLNC of the sciatic nerve and an ipsilateral intraganglionic MSC injection, using the von Frey and Choi tests. Control animals were subjected to the nerve lesion either alone or followed by the administration of phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs). All the animals were tested both before surgery and after 1, 3, 7, 14, 21, 28 and 56 days. Animals subjected to the sciatic nerve constriction developed ipsilateral mechanical and thermal allodynia already 3 days after the lesion. The allodynic responses were maintained even after 56 days. MSC administration prevented the generation of mechanical allodynia and reduced the number of allodynic responses to cold stimuli. On the contrary, the injection of either PBS or BNMCs could not counteract allodynia. These results suggest that MSCs may modulate pain generation after sciatic nerve constriction. The underlying mechanisms by which MSCs exert their actions on pain behavior need to be clarified.
Fil: Musolino, Patricia Leonor. Universidad Austral; Argentina
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Hökfelt, Tomas. Karolinska Institutet; Suecia
Fil: Villar, Marcelo José. Universidad Austral; Argentina
Materia
Pain
Bone Marrow Transplantation
Neuralgia
Sciatic Nerve
Stromal Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27524
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constrictionMusolino, Patricia LeonorCoronel, Maria FlorenciaHökfelt, TomasVillar, Marcelo JoséPainBone Marrow TransplantationNeuralgiaSciatic NerveStromal Cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Peripheral nerve injury, i.e. a single ligature nerve constriction (SLNC), triggers neuropathic pain. Bone marrow stromal cells (MSCs) have been observed to migrate to the injured tissues and mediate functional recovery following brain, spinal cord and peripheral nerve lesions. We have recently shown MSC selective migration to the ipsilateral lumbar (L3-6) dorsal root ganglia (DRGs) after a sciatic nerve SLNC. In this study, we have analyzed the thermal and mechanical sensitivities of animals subjected to a SLNC of the sciatic nerve and an ipsilateral intraganglionic MSC injection, using the von Frey and Choi tests. Control animals were subjected to the nerve lesion either alone or followed by the administration of phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs). All the animals were tested both before surgery and after 1, 3, 7, 14, 21, 28 and 56 days. Animals subjected to the sciatic nerve constriction developed ipsilateral mechanical and thermal allodynia already 3 days after the lesion. The allodynic responses were maintained even after 56 days. MSC administration prevented the generation of mechanical allodynia and reduced the number of allodynic responses to cold stimuli. On the contrary, the injection of either PBS or BNMCs could not counteract allodynia. These results suggest that MSCs may modulate pain generation after sciatic nerve constriction. The underlying mechanisms by which MSCs exert their actions on pain behavior need to be clarified.Fil: Musolino, Patricia Leonor. Universidad Austral; ArgentinaFil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Hökfelt, Tomas. Karolinska Institutet; SueciaFil: Villar, Marcelo José. Universidad Austral; ArgentinaElsevier Ireland2007-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27524Musolino, Patricia Leonor; Coronel, Maria Florencia; Hökfelt, Tomas; Villar, Marcelo José; Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction; Elsevier Ireland; Neuroscience Letters; 418; 1; 11-5-2007; 97-1010304-39401872-7972CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394007002704?via%3Dihub#!info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2007.03.001info:eu-repo/semantics/altIdentifier/pmid/17379405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:10Zoai:ri.conicet.gov.ar:11336/27524instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:11.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
title Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
spellingShingle Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
Musolino, Patricia Leonor
Pain
Bone Marrow Transplantation
Neuralgia
Sciatic Nerve
Stromal Cells
title_short Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
title_full Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
title_fullStr Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
title_full_unstemmed Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
title_sort Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction
dc.creator.none.fl_str_mv Musolino, Patricia Leonor
Coronel, Maria Florencia
Hökfelt, Tomas
Villar, Marcelo José
author Musolino, Patricia Leonor
author_facet Musolino, Patricia Leonor
Coronel, Maria Florencia
Hökfelt, Tomas
Villar, Marcelo José
author_role author
author2 Coronel, Maria Florencia
Hökfelt, Tomas
Villar, Marcelo José
author2_role author
author
author
dc.subject.none.fl_str_mv Pain
Bone Marrow Transplantation
Neuralgia
Sciatic Nerve
Stromal Cells
topic Pain
Bone Marrow Transplantation
Neuralgia
Sciatic Nerve
Stromal Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Peripheral nerve injury, i.e. a single ligature nerve constriction (SLNC), triggers neuropathic pain. Bone marrow stromal cells (MSCs) have been observed to migrate to the injured tissues and mediate functional recovery following brain, spinal cord and peripheral nerve lesions. We have recently shown MSC selective migration to the ipsilateral lumbar (L3-6) dorsal root ganglia (DRGs) after a sciatic nerve SLNC. In this study, we have analyzed the thermal and mechanical sensitivities of animals subjected to a SLNC of the sciatic nerve and an ipsilateral intraganglionic MSC injection, using the von Frey and Choi tests. Control animals were subjected to the nerve lesion either alone or followed by the administration of phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs). All the animals were tested both before surgery and after 1, 3, 7, 14, 21, 28 and 56 days. Animals subjected to the sciatic nerve constriction developed ipsilateral mechanical and thermal allodynia already 3 days after the lesion. The allodynic responses were maintained even after 56 days. MSC administration prevented the generation of mechanical allodynia and reduced the number of allodynic responses to cold stimuli. On the contrary, the injection of either PBS or BNMCs could not counteract allodynia. These results suggest that MSCs may modulate pain generation after sciatic nerve constriction. The underlying mechanisms by which MSCs exert their actions on pain behavior need to be clarified.
Fil: Musolino, Patricia Leonor. Universidad Austral; Argentina
Fil: Coronel, Maria Florencia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Hökfelt, Tomas. Karolinska Institutet; Suecia
Fil: Villar, Marcelo José. Universidad Austral; Argentina
description Peripheral nerve injury, i.e. a single ligature nerve constriction (SLNC), triggers neuropathic pain. Bone marrow stromal cells (MSCs) have been observed to migrate to the injured tissues and mediate functional recovery following brain, spinal cord and peripheral nerve lesions. We have recently shown MSC selective migration to the ipsilateral lumbar (L3-6) dorsal root ganglia (DRGs) after a sciatic nerve SLNC. In this study, we have analyzed the thermal and mechanical sensitivities of animals subjected to a SLNC of the sciatic nerve and an ipsilateral intraganglionic MSC injection, using the von Frey and Choi tests. Control animals were subjected to the nerve lesion either alone or followed by the administration of phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs). All the animals were tested both before surgery and after 1, 3, 7, 14, 21, 28 and 56 days. Animals subjected to the sciatic nerve constriction developed ipsilateral mechanical and thermal allodynia already 3 days after the lesion. The allodynic responses were maintained even after 56 days. MSC administration prevented the generation of mechanical allodynia and reduced the number of allodynic responses to cold stimuli. On the contrary, the injection of either PBS or BNMCs could not counteract allodynia. These results suggest that MSCs may modulate pain generation after sciatic nerve constriction. The underlying mechanisms by which MSCs exert their actions on pain behavior need to be clarified.
publishDate 2007
dc.date.none.fl_str_mv 2007-05-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27524
Musolino, Patricia Leonor; Coronel, Maria Florencia; Hökfelt, Tomas; Villar, Marcelo José; Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction; Elsevier Ireland; Neuroscience Letters; 418; 1; 11-5-2007; 97-101
0304-3940
1872-7972
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27524
identifier_str_mv Musolino, Patricia Leonor; Coronel, Maria Florencia; Hökfelt, Tomas; Villar, Marcelo José; Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction; Elsevier Ireland; Neuroscience Letters; 418; 1; 11-5-2007; 97-101
0304-3940
1872-7972
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304394007002704?via%3Dihub#!
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neulet.2007.03.001
info:eu-repo/semantics/altIdentifier/pmid/17379405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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