Rat bone marrow stromal cells and oligonucleotides in pain research
- Autores
- Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.
Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina - Fuente
- Protocol: Analgesia
- Materia
-
Sciatic Nerve Injury
Mechanical And Thermal Allodynia
Oligodeoxinucleotides
Bone Marrow Stromal Cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/14246
Ver los metadatos del registro completo
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Rat bone marrow stromal cells and oligonucleotides in pain researchCoronel, Maria FlorenciaChasseing, Norma AlejandraVillar, Marcelo JoseSciatic Nerve InjuryMechanical And Thermal AllodyniaOligodeoxinucleotidesBone Marrow Stromal CellsIn the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; ArgentinaFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; ArgentinaFil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; ArgentinaHumana Press2010-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14246Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-3361064-37451940-6029Protocol: Analgesiareponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicasenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-60327-323-7_24info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-60327-323-7_24info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/2025-09-03T10:10:19Zoai:ri.conicet.gov.ar:11336/14246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:19.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Rat bone marrow stromal cells and oligonucleotides in pain research |
title |
Rat bone marrow stromal cells and oligonucleotides in pain research |
spellingShingle |
Rat bone marrow stromal cells and oligonucleotides in pain research Coronel, Maria Florencia Sciatic Nerve Injury Mechanical And Thermal Allodynia Oligodeoxinucleotides Bone Marrow Stromal Cells |
title_short |
Rat bone marrow stromal cells and oligonucleotides in pain research |
title_full |
Rat bone marrow stromal cells and oligonucleotides in pain research |
title_fullStr |
Rat bone marrow stromal cells and oligonucleotides in pain research |
title_full_unstemmed |
Rat bone marrow stromal cells and oligonucleotides in pain research |
title_sort |
Rat bone marrow stromal cells and oligonucleotides in pain research |
dc.creator.none.fl_str_mv |
Coronel, Maria Florencia Chasseing, Norma Alejandra Villar, Marcelo Jose |
author |
Coronel, Maria Florencia |
author_facet |
Coronel, Maria Florencia Chasseing, Norma Alejandra Villar, Marcelo Jose |
author_role |
author |
author2 |
Chasseing, Norma Alejandra Villar, Marcelo Jose |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Sciatic Nerve Injury Mechanical And Thermal Allodynia Oligodeoxinucleotides Bone Marrow Stromal Cells |
topic |
Sciatic Nerve Injury Mechanical And Thermal Allodynia Oligodeoxinucleotides Bone Marrow Stromal Cells |
dc.description.none.fl_txt_mv |
In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities. Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina Fil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina |
description |
In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-13 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/14246 Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-336 1064-3745 1940-6029 |
url |
http://hdl.handle.net/11336/14246 |
identifier_str_mv |
Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-336 1064-3745 1940-6029 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-60327-323-7_24 info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-60327-323-7_24 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Humana Press |
publisher.none.fl_str_mv |
Humana Press |
dc.source.none.fl_str_mv |
Protocol: Analgesia reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270114386280448 |
score |
13.13397 |