Rat bone marrow stromal cells and oligonucleotides in pain research

Autores
Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.
Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
Fuente
Protocol: Analgesia
Materia
Sciatic Nerve Injury
Mechanical And Thermal Allodynia
Oligodeoxinucleotides
Bone Marrow Stromal Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14246
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14246
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rat bone marrow stromal cells and oligonucleotides in pain researchCoronel, Maria FlorenciaChasseing, Norma AlejandraVillar, Marcelo JoseSciatic Nerve InjuryMechanical And Thermal AllodyniaOligodeoxinucleotidesBone Marrow Stromal CellsIn the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; ArgentinaFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; ArgentinaFil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; ArgentinaHumana Press2010-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14246Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-3361064-37451940-6029Protocol: Analgesiareponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicasenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-60327-323-7_24info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-60327-323-7_24info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/2025-09-29T10:42:25Zoai:ri.conicet.gov.ar:11336/14246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:26.22CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rat bone marrow stromal cells and oligonucleotides in pain research
title Rat bone marrow stromal cells and oligonucleotides in pain research
spellingShingle Rat bone marrow stromal cells and oligonucleotides in pain research
Coronel, Maria Florencia
Sciatic Nerve Injury
Mechanical And Thermal Allodynia
Oligodeoxinucleotides
Bone Marrow Stromal Cells
title_short Rat bone marrow stromal cells and oligonucleotides in pain research
title_full Rat bone marrow stromal cells and oligonucleotides in pain research
title_fullStr Rat bone marrow stromal cells and oligonucleotides in pain research
title_full_unstemmed Rat bone marrow stromal cells and oligonucleotides in pain research
title_sort Rat bone marrow stromal cells and oligonucleotides in pain research
dc.creator.none.fl_str_mv Coronel, Maria Florencia
Chasseing, Norma Alejandra
Villar, Marcelo Jose
author Coronel, Maria Florencia
author_facet Coronel, Maria Florencia
Chasseing, Norma Alejandra
Villar, Marcelo Jose
author_role author
author2 Chasseing, Norma Alejandra
Villar, Marcelo Jose
author2_role author
author
dc.subject.none.fl_str_mv Sciatic Nerve Injury
Mechanical And Thermal Allodynia
Oligodeoxinucleotides
Bone Marrow Stromal Cells
topic Sciatic Nerve Injury
Mechanical And Thermal Allodynia
Oligodeoxinucleotides
Bone Marrow Stromal Cells
dc.description.none.fl_txt_mv In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.
Fil: Coronel, Maria Florencia. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina
Fil: Villar, Marcelo Jose. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina
description In the last years, significant progress has been made in the medical treatment of pain. However, pathological pains, such us neuropathic pain, remain refractory to the currently available analgesics. Therefore, new therapeutic strategies are being evaluated. We have recently shown that both bone marrow stromal cells (MSCs) and the oligonucleotide IMT504 can prevent the development of mechanical and thermal allodynia when they are administered to rats subjected to a sciatic nerve crush. This chapter summarizes the laboratory techniques used to isolate and culture MSCs, administer both MSCs and IMT504, perform the nerve injury and determine mechanical and thermal sensitivities.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14246
Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-336
1064-3745
1940-6029
url http://hdl.handle.net/11336/14246
identifier_str_mv Coronel, Maria Florencia; Chasseing, Norma Alejandra; Villar, Marcelo Jose; Rat bone marrow stromal cells and oligonucleotides in pain research; Humana Press; Methods In Molecular Biology (clifton, N.j.); 617; 13-2-2010; 327-336
1064-3745
1940-6029
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-60327-323-7_24
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-60327-323-7_24
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv Protocol: Analgesia
reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614457025626112
score 13.070432

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