Actinobacteria resistant to contaminants of emerging concern

Autores
Gonzalez Holc, Victoria Guadalupe; Aparicio, Juan Daniel; Costa Gutierrez, Stefanie Bernardette; Raimondo, Enzo Emanuel; Polti, Marta Alejandra
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Contaminants of Emerging Concern (CECs) are synthetic or naturally occurring chemicals that are not commonly monitored in the environment, but have the potential to enter the environment and cause adverse ecological and/or health effects. A promising technology to clean up environments contaminated with CECs is bioremediation using actinobacteria, which are microorganisms with great metabolic diversity and ability to detoxify different organic and inorganic compounds. In this context, the objective of the present work was to select actinobacteria resistant to CECs of regional relevance. The resistance to CECs of 12 actinobacterial strains, previously isolated from contaminated environments, was qualitatively evaluated. The CECs studied were Diclofenac (DIC), Sildenafil (SIL) and Ivermectin (IVE). These CECs were selected because they were detected in several domestic and hospital effluents in the northwestern region of Argentina, and they belong to different chemical groups. The qualitative screening assay was carried out in Petri dish plates containing 20 ml of casein starch agar medium (CSA). For the DIC and SIL assays, rectangular troughs (1.5 x 6 cm) were cut in the centre of plates and filled with 1 mL of the solution to be tested. For the IVE assays, due to its insolubility, the solution was added directly to the CSA medium. The concentrations tested were: 1, 5 and 10 mg/mL. The strains were inoculated perpendicular to the rectangular troughs (DIC, SIL) or equidistant (IVE). Plates were incubated at 30 ºC for 7 days. Control plates were also performed, using sterile distilled water instead of CECs. For each strain, growth, spore formation and pigment production were evaluated in comparison to that observed on control plates. For DIC and SIL, 2 strains, different for each CEC, were able to grow, form spores and produce pigments, at levels comparable to their corresponding controls. For IVE, 12 strains were able to grow and produce pigments, but only 9 of them formed spores. None of the strains studied showed tolerance to the three CECs, although several strains showed tolerance to 2 CECs. These results demonstrate the great potential of actinobacteria to grow in presence of several types of CECs, also indicating that the metabolic pathways involved in each type of tolerance may be different. The strains with the highest resistance to each CEC were selected for degradation tests in liquid culture media, in order to evaluate their ability to use the CEC as the only source of carbon and energy.
Fil: Gonzalez Holc, Victoria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Materia
Actinobacteria
Emerging concern
Bioremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210386
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Actinobacteria resistant to contaminants of emerging concernGonzalez Holc, Victoria GuadalupeAparicio, Juan DanielCosta Gutierrez, Stefanie BernardetteRaimondo, Enzo EmanuelPolti, Marta AlejandraActinobacteriaEmerging concernBioremediationhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The Contaminants of Emerging Concern (CECs) are synthetic or naturally occurring chemicals that are not commonly monitored in the environment, but have the potential to enter the environment and cause adverse ecological and/or health effects. A promising technology to clean up environments contaminated with CECs is bioremediation using actinobacteria, which are microorganisms with great metabolic diversity and ability to detoxify different organic and inorganic compounds. In this context, the objective of the present work was to select actinobacteria resistant to CECs of regional relevance. The resistance to CECs of 12 actinobacterial strains, previously isolated from contaminated environments, was qualitatively evaluated. The CECs studied were Diclofenac (DIC), Sildenafil (SIL) and Ivermectin (IVE). These CECs were selected because they were detected in several domestic and hospital effluents in the northwestern region of Argentina, and they belong to different chemical groups. The qualitative screening assay was carried out in Petri dish plates containing 20 ml of casein starch agar medium (CSA). For the DIC and SIL assays, rectangular troughs (1.5 x 6 cm) were cut in the centre of plates and filled with 1 mL of the solution to be tested. For the IVE assays, due to its insolubility, the solution was added directly to the CSA medium. The concentrations tested were: 1, 5 and 10 mg/mL. The strains were inoculated perpendicular to the rectangular troughs (DIC, SIL) or equidistant (IVE). Plates were incubated at 30 ºC for 7 days. Control plates were also performed, using sterile distilled water instead of CECs. For each strain, growth, spore formation and pigment production were evaluated in comparison to that observed on control plates. For DIC and SIL, 2 strains, different for each CEC, were able to grow, form spores and produce pigments, at levels comparable to their corresponding controls. For IVE, 12 strains were able to grow and produce pigments, but only 9 of them formed spores. None of the strains studied showed tolerance to the three CECs, although several strains showed tolerance to 2 CECs. These results demonstrate the great potential of actinobacteria to grow in presence of several types of CECs, also indicating that the metabolic pathways involved in each type of tolerance may be different. The strains with the highest resistance to each CEC were selected for degradation tests in liquid culture media, in order to evaluate their ability to use the CEC as the only source of carbon and energy.Fil: Gonzalez Holc, Victoria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaXL Reunión Científica Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210386Actinobacteria resistant to contaminants of emerging concern; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 105-105CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:49:02Zoai:ri.conicet.gov.ar:11336/210386instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:49:02.423CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Actinobacteria resistant to contaminants of emerging concern
title Actinobacteria resistant to contaminants of emerging concern
spellingShingle Actinobacteria resistant to contaminants of emerging concern
Gonzalez Holc, Victoria Guadalupe
Actinobacteria
Emerging concern
Bioremediation
title_short Actinobacteria resistant to contaminants of emerging concern
title_full Actinobacteria resistant to contaminants of emerging concern
title_fullStr Actinobacteria resistant to contaminants of emerging concern
title_full_unstemmed Actinobacteria resistant to contaminants of emerging concern
title_sort Actinobacteria resistant to contaminants of emerging concern
dc.creator.none.fl_str_mv Gonzalez Holc, Victoria Guadalupe
Aparicio, Juan Daniel
Costa Gutierrez, Stefanie Bernardette
Raimondo, Enzo Emanuel
Polti, Marta Alejandra
author Gonzalez Holc, Victoria Guadalupe
author_facet Gonzalez Holc, Victoria Guadalupe
Aparicio, Juan Daniel
Costa Gutierrez, Stefanie Bernardette
Raimondo, Enzo Emanuel
Polti, Marta Alejandra
author_role author
author2 Aparicio, Juan Daniel
Costa Gutierrez, Stefanie Bernardette
Raimondo, Enzo Emanuel
Polti, Marta Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Actinobacteria
Emerging concern
Bioremediation
topic Actinobacteria
Emerging concern
Bioremediation
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The Contaminants of Emerging Concern (CECs) are synthetic or naturally occurring chemicals that are not commonly monitored in the environment, but have the potential to enter the environment and cause adverse ecological and/or health effects. A promising technology to clean up environments contaminated with CECs is bioremediation using actinobacteria, which are microorganisms with great metabolic diversity and ability to detoxify different organic and inorganic compounds. In this context, the objective of the present work was to select actinobacteria resistant to CECs of regional relevance. The resistance to CECs of 12 actinobacterial strains, previously isolated from contaminated environments, was qualitatively evaluated. The CECs studied were Diclofenac (DIC), Sildenafil (SIL) and Ivermectin (IVE). These CECs were selected because they were detected in several domestic and hospital effluents in the northwestern region of Argentina, and they belong to different chemical groups. The qualitative screening assay was carried out in Petri dish plates containing 20 ml of casein starch agar medium (CSA). For the DIC and SIL assays, rectangular troughs (1.5 x 6 cm) were cut in the centre of plates and filled with 1 mL of the solution to be tested. For the IVE assays, due to its insolubility, the solution was added directly to the CSA medium. The concentrations tested were: 1, 5 and 10 mg/mL. The strains were inoculated perpendicular to the rectangular troughs (DIC, SIL) or equidistant (IVE). Plates were incubated at 30 ºC for 7 days. Control plates were also performed, using sterile distilled water instead of CECs. For each strain, growth, spore formation and pigment production were evaluated in comparison to that observed on control plates. For DIC and SIL, 2 strains, different for each CEC, were able to grow, form spores and produce pigments, at levels comparable to their corresponding controls. For IVE, 12 strains were able to grow and produce pigments, but only 9 of them formed spores. None of the strains studied showed tolerance to the three CECs, although several strains showed tolerance to 2 CECs. These results demonstrate the great potential of actinobacteria to grow in presence of several types of CECs, also indicating that the metabolic pathways involved in each type of tolerance may be different. The strains with the highest resistance to each CEC were selected for degradation tests in liquid culture media, in order to evaluate their ability to use the CEC as the only source of carbon and energy.
Fil: Gonzalez Holc, Victoria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Costa Gutierrez, Stefanie Bernardette. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Raimondo, Enzo Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Polti, Marta Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
description The Contaminants of Emerging Concern (CECs) are synthetic or naturally occurring chemicals that are not commonly monitored in the environment, but have the potential to enter the environment and cause adverse ecological and/or health effects. A promising technology to clean up environments contaminated with CECs is bioremediation using actinobacteria, which are microorganisms with great metabolic diversity and ability to detoxify different organic and inorganic compounds. In this context, the objective of the present work was to select actinobacteria resistant to CECs of regional relevance. The resistance to CECs of 12 actinobacterial strains, previously isolated from contaminated environments, was qualitatively evaluated. The CECs studied were Diclofenac (DIC), Sildenafil (SIL) and Ivermectin (IVE). These CECs were selected because they were detected in several domestic and hospital effluents in the northwestern region of Argentina, and they belong to different chemical groups. The qualitative screening assay was carried out in Petri dish plates containing 20 ml of casein starch agar medium (CSA). For the DIC and SIL assays, rectangular troughs (1.5 x 6 cm) were cut in the centre of plates and filled with 1 mL of the solution to be tested. For the IVE assays, due to its insolubility, the solution was added directly to the CSA medium. The concentrations tested were: 1, 5 and 10 mg/mL. The strains were inoculated perpendicular to the rectangular troughs (DIC, SIL) or equidistant (IVE). Plates were incubated at 30 ºC for 7 days. Control plates were also performed, using sterile distilled water instead of CECs. For each strain, growth, spore formation and pigment production were evaluated in comparison to that observed on control plates. For DIC and SIL, 2 strains, different for each CEC, were able to grow, form spores and produce pigments, at levels comparable to their corresponding controls. For IVE, 12 strains were able to grow and produce pigments, but only 9 of them formed spores. None of the strains studied showed tolerance to the three CECs, although several strains showed tolerance to 2 CECs. These results demonstrate the great potential of actinobacteria to grow in presence of several types of CECs, also indicating that the metabolic pathways involved in each type of tolerance may be different. The strains with the highest resistance to each CEC were selected for degradation tests in liquid culture media, in order to evaluate their ability to use the CEC as the only source of carbon and energy.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210386
Actinobacteria resistant to contaminants of emerging concern; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 105-105
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210386
identifier_str_mv Actinobacteria resistant to contaminants of emerging concern; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 105-105
CONICET Digital
CONICET
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language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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