Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
- Autores
- Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; Cortiñas, Teresa Inés; Silva, Humberto de Jesus
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.
Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina - Materia
-
HELICOBACTER
BIOFILM
CYANOBACTERIAL
GENE-EXPRESSION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/270794
Ver los metadatos del registro completo
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Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial ExtractVega, Alba EdithPersia, AndresSalinas Ibañez, Angel GabrielAlarcón, María TeresaLucero Estrada, Cecilia Stella MarysCortiñas, Teresa InésSilva, Humberto de JesusHELICOBACTERBIOFILMCYANOBACTERIALGENE-EXPRESSIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; EspañaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaDavid Publishing Company2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270794Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-13271934-7391CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.davidpublisher.com/Home/Journal/JLSinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:37Zoai:ri.conicet.gov.ar:11336/270794instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:37.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
title |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
spellingShingle |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract Vega, Alba Edith HELICOBACTER BIOFILM CYANOBACTERIAL GENE-EXPRESSION |
title_short |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
title_full |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
title_fullStr |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
title_full_unstemmed |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
title_sort |
Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract |
dc.creator.none.fl_str_mv |
Vega, Alba Edith Persia, Andres Salinas Ibañez, Angel Gabriel Alarcón, María Teresa Lucero Estrada, Cecilia Stella Marys Cortiñas, Teresa Inés Silva, Humberto de Jesus |
author |
Vega, Alba Edith |
author_facet |
Vega, Alba Edith Persia, Andres Salinas Ibañez, Angel Gabriel Alarcón, María Teresa Lucero Estrada, Cecilia Stella Marys Cortiñas, Teresa Inés Silva, Humberto de Jesus |
author_role |
author |
author2 |
Persia, Andres Salinas Ibañez, Angel Gabriel Alarcón, María Teresa Lucero Estrada, Cecilia Stella Marys Cortiñas, Teresa Inés Silva, Humberto de Jesus |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
HELICOBACTER BIOFILM CYANOBACTERIAL GENE-EXPRESSION |
topic |
HELICOBACTER BIOFILM CYANOBACTERIAL GENE-EXPRESSION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments. Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina |
description |
The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/270794 Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-1327 1934-7391 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/270794 |
identifier_str_mv |
Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-1327 1934-7391 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.davidpublisher.com/Home/Journal/JLS |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
David Publishing Company |
publisher.none.fl_str_mv |
David Publishing Company |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613916688121856 |
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13.070432 |