Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract

Autores
Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; Cortiñas, Teresa Inés; Silva, Humberto de Jesus
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.
Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
HELICOBACTER
BIOFILM
CYANOBACTERIAL
GENE-EXPRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270794

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network_name_str CONICET Digital (CONICET)
spelling Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial ExtractVega, Alba EdithPersia, AndresSalinas Ibañez, Angel GabrielAlarcón, María TeresaLucero Estrada, Cecilia Stella MarysCortiñas, Teresa InésSilva, Humberto de JesusHELICOBACTERBIOFILMCYANOBACTERIALGENE-EXPRESSIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; EspañaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaDavid Publishing Company2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270794Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-13271934-7391CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.davidpublisher.com/Home/Journal/JLSinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:37Zoai:ri.conicet.gov.ar:11336/270794instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:37.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
title Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
spellingShingle Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
Vega, Alba Edith
HELICOBACTER
BIOFILM
CYANOBACTERIAL
GENE-EXPRESSION
title_short Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
title_full Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
title_fullStr Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
title_full_unstemmed Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
title_sort Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract
dc.creator.none.fl_str_mv Vega, Alba Edith
Persia, Andres
Salinas Ibañez, Angel Gabriel
Alarcón, María Teresa
Lucero Estrada, Cecilia Stella Marys
Cortiñas, Teresa Inés
Silva, Humberto de Jesus
author Vega, Alba Edith
author_facet Vega, Alba Edith
Persia, Andres
Salinas Ibañez, Angel Gabriel
Alarcón, María Teresa
Lucero Estrada, Cecilia Stella Marys
Cortiñas, Teresa Inés
Silva, Humberto de Jesus
author_role author
author2 Persia, Andres
Salinas Ibañez, Angel Gabriel
Alarcón, María Teresa
Lucero Estrada, Cecilia Stella Marys
Cortiñas, Teresa Inés
Silva, Humberto de Jesus
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HELICOBACTER
BIOFILM
CYANOBACTERIAL
GENE-EXPRESSION
topic HELICOBACTER
BIOFILM
CYANOBACTERIAL
GENE-EXPRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.
Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Salinas Ibañez, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Alarcón, María Teresa. Universidad Autonoma de Madrid. Hospital Universitario de la Princesa; España
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description The effects of a cyanobacterial extract (CE) on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in Mueller-Hinton broth supplemented with 5% fetal calf serum (FCS) or 1% CE. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with CE. Cells attachment was higher with CE than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the omp18 gene using the CE supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by CE favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270794
Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-1327
1934-7391
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270794
identifier_str_mv Vega, Alba Edith; Persia, Andres; Salinas Ibañez, Angel Gabriel; Alarcón, María Teresa; Lucero Estrada, Cecilia Stella Marys; et al.; Helicobacter pylori Biofilm Formation and Gene Expression on Abiotic Surfaces Using a Cyanobacterial Extract; David Publishing Company; Journal of Life Sciences; 6; 12-2012; 1317-1327
1934-7391
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.davidpublisher.com/Home/Journal/JLS
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv David Publishing Company
publisher.none.fl_str_mv David Publishing Company
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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