Volatilomic signatures of different strains of Helicobacter pylori

Autores
Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; Corvalan, Alejandro H.; Fernandez, Elmer Andres; Mayhew, Chris A.; Leja, Marcis; Mochalski, Pawel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.
Fil: Vangravs, Reinis. Universidad de Letonia; Letonia
Fil: Mezmale, Linda. Universidad de Letonia; Letonia
Fil: Slefarska Wolak, Daria. University of Innsbruck; Austria
Fil: Dauss, Edgars. Universidad de Letonia; Letonia
Fil: Ager, Clemens. Universidad de Letonia; Letonia
Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; Argentina
Fil: Mayhew, Chris A.. Universidad de Innsbruck; Austria
Fil: Leja, Marcis. Universidad de Letonia; Letonia
Fil: Mochalski, Pawel. Universidad de Innsbruck; Austria
Materia
cancer
gastrico
helicobacter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231586

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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Volatilomic signatures of different strains of Helicobacter pyloriVangravs, ReinisMezmale, LindaSlefarska Wolak, DariaDauss, EdgarsAger, ClemensCorvalan, Alejandro H.Fernandez, Elmer AndresMayhew, Chris A.Leja, MarcisMochalski, Pawelcancergastricohelicobacterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.Fil: Vangravs, Reinis. Universidad de Letonia; LetoniaFil: Mezmale, Linda. Universidad de Letonia; LetoniaFil: Slefarska Wolak, Daria. University of Innsbruck; AustriaFil: Dauss, Edgars. Universidad de Letonia; LetoniaFil: Ager, Clemens. Universidad de Letonia; LetoniaFil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; ChileFil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; ArgentinaFil: Mayhew, Chris A.. Universidad de Innsbruck; AustriaFil: Leja, Marcis. Universidad de Letonia; LetoniaFil: Mochalski, Pawel. Universidad de Innsbruck; AustriaWiley Blackwell Publishing, Inc2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231586Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-111083-43891523-5378CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/hel.13064info:eu-repo/semantics/altIdentifier/doi/10.1111/hel.13064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:19Zoai:ri.conicet.gov.ar:11336/231586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:19.305CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Volatilomic signatures of different strains of Helicobacter pylori
title Volatilomic signatures of different strains of Helicobacter pylori
spellingShingle Volatilomic signatures of different strains of Helicobacter pylori
Vangravs, Reinis
cancer
gastrico
helicobacter
title_short Volatilomic signatures of different strains of Helicobacter pylori
title_full Volatilomic signatures of different strains of Helicobacter pylori
title_fullStr Volatilomic signatures of different strains of Helicobacter pylori
title_full_unstemmed Volatilomic signatures of different strains of Helicobacter pylori
title_sort Volatilomic signatures of different strains of Helicobacter pylori
dc.creator.none.fl_str_mv Vangravs, Reinis
Mezmale, Linda
Slefarska Wolak, Daria
Dauss, Edgars
Ager, Clemens
Corvalan, Alejandro H.
Fernandez, Elmer Andres
Mayhew, Chris A.
Leja, Marcis
Mochalski, Pawel
author Vangravs, Reinis
author_facet Vangravs, Reinis
Mezmale, Linda
Slefarska Wolak, Daria
Dauss, Edgars
Ager, Clemens
Corvalan, Alejandro H.
Fernandez, Elmer Andres
Mayhew, Chris A.
Leja, Marcis
Mochalski, Pawel
author_role author
author2 Mezmale, Linda
Slefarska Wolak, Daria
Dauss, Edgars
Ager, Clemens
Corvalan, Alejandro H.
Fernandez, Elmer Andres
Mayhew, Chris A.
Leja, Marcis
Mochalski, Pawel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cancer
gastrico
helicobacter
topic cancer
gastrico
helicobacter
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.
Fil: Vangravs, Reinis. Universidad de Letonia; Letonia
Fil: Mezmale, Linda. Universidad de Letonia; Letonia
Fil: Slefarska Wolak, Daria. University of Innsbruck; Austria
Fil: Dauss, Edgars. Universidad de Letonia; Letonia
Fil: Ager, Clemens. Universidad de Letonia; Letonia
Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; Argentina
Fil: Mayhew, Chris A.. Universidad de Innsbruck; Austria
Fil: Leja, Marcis. Universidad de Letonia; Letonia
Fil: Mochalski, Pawel. Universidad de Innsbruck; Austria
description Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231586
Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-11
1083-4389
1523-5378
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231586
identifier_str_mv Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-11
1083-4389
1523-5378
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/hel.13064
info:eu-repo/semantics/altIdentifier/doi/10.1111/hel.13064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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