Volatilomic signatures of different strains of Helicobacter pylori
- Autores
- Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; Corvalan, Alejandro H.; Fernandez, Elmer Andres; Mayhew, Chris A.; Leja, Marcis; Mochalski, Pawel
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.
Fil: Vangravs, Reinis. Universidad de Letonia; Letonia
Fil: Mezmale, Linda. Universidad de Letonia; Letonia
Fil: Slefarska Wolak, Daria. University of Innsbruck; Austria
Fil: Dauss, Edgars. Universidad de Letonia; Letonia
Fil: Ager, Clemens. Universidad de Letonia; Letonia
Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; Argentina
Fil: Mayhew, Chris A.. Universidad de Innsbruck; Austria
Fil: Leja, Marcis. Universidad de Letonia; Letonia
Fil: Mochalski, Pawel. Universidad de Innsbruck; Austria - Materia
-
cancer
gastrico
helicobacter - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/231586
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oai:ri.conicet.gov.ar:11336/231586 |
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Volatilomic signatures of different strains of Helicobacter pyloriVangravs, ReinisMezmale, LindaSlefarska Wolak, DariaDauss, EdgarsAger, ClemensCorvalan, Alejandro H.Fernandez, Elmer AndresMayhew, Chris A.Leja, MarcisMochalski, Pawelcancergastricohelicobacterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.Fil: Vangravs, Reinis. Universidad de Letonia; LetoniaFil: Mezmale, Linda. Universidad de Letonia; LetoniaFil: Slefarska Wolak, Daria. University of Innsbruck; AustriaFil: Dauss, Edgars. Universidad de Letonia; LetoniaFil: Ager, Clemens. Universidad de Letonia; LetoniaFil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; ChileFil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; ArgentinaFil: Mayhew, Chris A.. Universidad de Innsbruck; AustriaFil: Leja, Marcis. Universidad de Letonia; LetoniaFil: Mochalski, Pawel. Universidad de Innsbruck; AustriaWiley Blackwell Publishing, Inc2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231586Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-111083-43891523-5378CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/hel.13064info:eu-repo/semantics/altIdentifier/doi/10.1111/hel.13064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:19Zoai:ri.conicet.gov.ar:11336/231586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:19.305CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Volatilomic signatures of different strains of Helicobacter pylori |
title |
Volatilomic signatures of different strains of Helicobacter pylori |
spellingShingle |
Volatilomic signatures of different strains of Helicobacter pylori Vangravs, Reinis cancer gastrico helicobacter |
title_short |
Volatilomic signatures of different strains of Helicobacter pylori |
title_full |
Volatilomic signatures of different strains of Helicobacter pylori |
title_fullStr |
Volatilomic signatures of different strains of Helicobacter pylori |
title_full_unstemmed |
Volatilomic signatures of different strains of Helicobacter pylori |
title_sort |
Volatilomic signatures of different strains of Helicobacter pylori |
dc.creator.none.fl_str_mv |
Vangravs, Reinis Mezmale, Linda Slefarska Wolak, Daria Dauss, Edgars Ager, Clemens Corvalan, Alejandro H. Fernandez, Elmer Andres Mayhew, Chris A. Leja, Marcis Mochalski, Pawel |
author |
Vangravs, Reinis |
author_facet |
Vangravs, Reinis Mezmale, Linda Slefarska Wolak, Daria Dauss, Edgars Ager, Clemens Corvalan, Alejandro H. Fernandez, Elmer Andres Mayhew, Chris A. Leja, Marcis Mochalski, Pawel |
author_role |
author |
author2 |
Mezmale, Linda Slefarska Wolak, Daria Dauss, Edgars Ager, Clemens Corvalan, Alejandro H. Fernandez, Elmer Andres Mayhew, Chris A. Leja, Marcis Mochalski, Pawel |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
cancer gastrico helicobacter |
topic |
cancer gastrico helicobacter |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers. Fil: Vangravs, Reinis. Universidad de Letonia; Letonia Fil: Mezmale, Linda. Universidad de Letonia; Letonia Fil: Slefarska Wolak, Daria. University of Innsbruck; Austria Fil: Dauss, Edgars. Universidad de Letonia; Letonia Fil: Ager, Clemens. Universidad de Letonia; Letonia Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Fundación para el Progreso de la Medicina; Argentina Fil: Mayhew, Chris A.. Universidad de Innsbruck; Austria Fil: Leja, Marcis. Universidad de Letonia; Letonia Fil: Mochalski, Pawel. Universidad de Innsbruck; Austria |
description |
Background: Helicobacter pylori (H. pylori) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, H. pylori will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non-invasive diagnostic methods for determining infection. Here we characterize the H. pylori volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different H. pylori isolates. Materials and Methods: Using needle trap extraction, volatiles in the headspace above H. pylori cultures were collected and, following thermal desorption at 290°C in a splitless mode, were analyzed using gas chromatography–mass spectrometry. The resulting volatilomic signatures of H. pylori cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only. Results: Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2-pentanone, 5-methyl-3-heptanone, 2-heptanone, and 2-nonanone), three alcohols (2-methyl-1-propanol, 3-methyl-1-butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2-ethyl-hexanal), one hydrocarbon (noctane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2-methylpropanal, benzaldehyde, 3-methylbutanal, and butanal), two heterocyclic compounds (2-ethylfuran and 2-pentylfuran), one ketone (2-butanone), one aromatic (benzene), one alcohol (2-butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3-methyl-1-butanol, 1-butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed. Conclusions: The volatiles emitted by H. pylori provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of H. pylori. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/231586 Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-11 1083-4389 1523-5378 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/231586 |
identifier_str_mv |
Vangravs, Reinis; Mezmale, Linda; Slefarska Wolak, Daria; Dauss, Edgars; Ager, Clemens; et al.; Volatilomic signatures of different strains of Helicobacter pylori; Wiley Blackwell Publishing, Inc; Helicobacter; 29; 2; 3-2024; 1-11 1083-4389 1523-5378 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/hel.13064 info:eu-repo/semantics/altIdentifier/doi/10.1111/hel.13064 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614251558207488 |
score |
13.070432 |