ComB proteins expression levels determine Helicobacter pylori competence capacity

Autores
Corbinais, Christopher; Mathieu, Aurélie; Damke, Prashant P.; Kortulewski, Thierry; Busso, Didier; Prado Acosta, Mariano; Radicella, J. Pablo; Marsin, Stéphanie
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Helicobacter pylori chronically colonises half of the world's human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency.
Fil: Corbinais, Christopher. Inserm; Francia. Institute of Molecular and Cellular Radiobiology; Francia. Université Paris Sud; Francia
Fil: Mathieu, Aurélie. Institute of Molecular and Cellular Radiobiology; Francia
Fil: Damke, Prashant P.. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Kortulewski, Thierry. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Busso, Didier. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Prado Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institute of Molecular and Cellular Radiobiology; Francia
Fil: Radicella, J. Pablo. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Marsin, Stéphanie. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Materia
Helicobacter Pylori
Transformation
Dna
Competence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84358
Acceder
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network_name_str CONICET Digital (CONICET)
spelling ComB proteins expression levels determine Helicobacter pylori competence capacityCorbinais, ChristopherMathieu, AurélieDamke, Prashant P.Kortulewski, ThierryBusso, DidierPrado Acosta, MarianoRadicella, J. PabloMarsin, StéphanieHelicobacter PyloriTransformationDnaCompetencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Helicobacter pylori chronically colonises half of the world's human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency.Fil: Corbinais, Christopher. Inserm; Francia. Institute of Molecular and Cellular Radiobiology; Francia. Université Paris Sud; FranciaFil: Mathieu, Aurélie. Institute of Molecular and Cellular Radiobiology; FranciaFil: Damke, Prashant P.. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; FranciaFil: Kortulewski, Thierry. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; FranciaFil: Busso, Didier. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; FranciaFil: Prado Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institute of Molecular and Cellular Radiobiology; FranciaFil: Radicella, J. Pablo. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; FranciaFil: Marsin, Stéphanie. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; FranciaNature Publishing Group2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84358Corbinais, Christopher; Mathieu, Aurélie; Damke, Prashant P.; Kortulewski, Thierry; Busso, Didier; et al.; ComB proteins expression levels determine Helicobacter pylori competence capacity; Nature Publishing Group; Scientific Reports; 7; 4149; 1-2017; 1-92045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep41495info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep41495info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:21Zoai:ri.conicet.gov.ar:11336/84358instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:22.152CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ComB proteins expression levels determine Helicobacter pylori competence capacity
title ComB proteins expression levels determine Helicobacter pylori competence capacity
spellingShingle ComB proteins expression levels determine Helicobacter pylori competence capacity
Corbinais, Christopher
Helicobacter Pylori
Transformation
Dna
Competence
title_short ComB proteins expression levels determine Helicobacter pylori competence capacity
title_full ComB proteins expression levels determine Helicobacter pylori competence capacity
title_fullStr ComB proteins expression levels determine Helicobacter pylori competence capacity
title_full_unstemmed ComB proteins expression levels determine Helicobacter pylori competence capacity
title_sort ComB proteins expression levels determine Helicobacter pylori competence capacity
dc.creator.none.fl_str_mv Corbinais, Christopher
Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
author Corbinais, Christopher
author_facet Corbinais, Christopher
Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
author_role author
author2 Mathieu, Aurélie
Damke, Prashant P.
Kortulewski, Thierry
Busso, Didier
Prado Acosta, Mariano
Radicella, J. Pablo
Marsin, Stéphanie
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Helicobacter Pylori
Transformation
Dna
Competence
topic Helicobacter Pylori
Transformation
Dna
Competence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Helicobacter pylori chronically colonises half of the world's human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency.
Fil: Corbinais, Christopher. Inserm; Francia. Institute of Molecular and Cellular Radiobiology; Francia. Université Paris Sud; Francia
Fil: Mathieu, Aurélie. Institute of Molecular and Cellular Radiobiology; Francia
Fil: Damke, Prashant P.. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Kortulewski, Thierry. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Busso, Didier. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Prado Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institute of Molecular and Cellular Radiobiology; Francia
Fil: Radicella, J. Pablo. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
Fil: Marsin, Stéphanie. Institute of Molecular and Cellular Radiobiology; Francia. Inserm; Francia. Université Paris Sud; Francia
description Helicobacter pylori chronically colonises half of the world's human population and is the main cause of ulcers and gastric cancers. Its prevalence and the increase in antibiotic resistance observed recently reflect the high genetic adaptability of this pathogen. Together with high mutation rates and an efficient DNA recombination system, horizontal gene transfer through natural competence makes of H. pylori one of the most genetically diverse bacteria. We show here that transformation capacity is enhanced in strains defective for recN, extending previous work with other homologous recombination genes. However, inactivation of either mutY or polA has no effect on DNA transformation, suggesting that natural competence can be boosted in H. pylori by the persistence of DNA breaks but not by enhanced mutagenesis. The transformation efficiency of the different DNA repair impaired strains correlates with the number of transforming DNA foci formed on the cell surface and with the expression of comB8 and comB10 competence genes. Overexpression of the comB6-B10 operon is sufficient to increase the transformation capacity of a wild type strain, indicating that the ComB complex, present in the bacterial wall and essential for DNA uptake, can be a limiting factor for transformation efficiency.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84358
Corbinais, Christopher; Mathieu, Aurélie; Damke, Prashant P.; Kortulewski, Thierry; Busso, Didier; et al.; ComB proteins expression levels determine Helicobacter pylori competence capacity; Nature Publishing Group; Scientific Reports; 7; 4149; 1-2017; 1-9
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84358
identifier_str_mv Corbinais, Christopher; Mathieu, Aurélie; Damke, Prashant P.; Kortulewski, Thierry; Busso, Didier; et al.; ComB proteins expression levels determine Helicobacter pylori competence capacity; Nature Publishing Group; Scientific Reports; 7; 4149; 1-2017; 1-9
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep41495
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep41495
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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