Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina

Autores
Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de Jesus
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To characterize Helicobacter pylori isolates from western Argentina using virulence markers and antimicrobial susceptibility patterns in order to assess the association between virulent genotypes, antimicrobial resistance, and disease. DNA fingerprinting was also evaluated for the segregation of virulent or resistant strain clusters. Methods: Genotyping of 299 H. pylori isolates was performed by PCR using specific primers for the cagA,vacA and iceA genes. Random amplification of polymorphic DNA (RAPD)-PCR and rep-PCR genetic clustering were assessed using five random primers and BOXA1R and ERIC primers, respectively. Resistance to clarithromycin (Cla) and metronidazole (Mtz) was assessed by the agar dilution method.Results: It was observed that 40.8% of the genotypes were cagA-positive; 66.9% were vacA s1m1 genotype and the iceA1 allele was found in 40.8%. A significant correlation (p = 0.0000) was observed between cagA positivity and vacA s1m1/iceA1 genotypes. Triple virulent genotypes were statistically associated with peptic ulcer (PU) (p = 0.0001) and Cla resistance (p = 0.0000). RAPD fingerprints obtained with AO2 primers identified clusters that were strongly associated with PU, virulence markers, and resistance to Cla and Mtz.Conclusions: The H. pylori isolates that harbored two or three virulence markers were more resistant to Cla and Mtz. Combined analysis of virulent genotypes and resistance patterns may permit identification of high-risk patients to prevent PU later in life or to avoid antimicrobial treatment failure.
Fil: Vega, Alba Edith. Universidad Nacional de San Luis; Argentina
Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis; Argentina
Fil: Centrobi, Olga Nélida. Universidad Nacional de San Luis; Argentina
Fil: Silva, Humberto de Jesus. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; Argentina
Materia
Helicobacter
Antimicrobial
Virulence
Rapd-Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13972
Acceder
id CONICETDig_7d75950f7e5b95747b566de1cec4f76b
oai_identifier_str oai:ri.conicet.gov.ar:11336/13972
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western ArgentinaVega, Alba EdithCortiñas, Teresa InésCentrobi, Olga NélidaSilva, Humberto de JesusHelicobacterAntimicrobialVirulenceRapd-Pcrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: To characterize Helicobacter pylori isolates from western Argentina using virulence markers and antimicrobial susceptibility patterns in order to assess the association between virulent genotypes, antimicrobial resistance, and disease. DNA fingerprinting was also evaluated for the segregation of virulent or resistant strain clusters. Methods: Genotyping of 299 H. pylori isolates was performed by PCR using specific primers for the cagA,vacA and iceA genes. Random amplification of polymorphic DNA (RAPD)-PCR and rep-PCR genetic clustering were assessed using five random primers and BOXA1R and ERIC primers, respectively. Resistance to clarithromycin (Cla) and metronidazole (Mtz) was assessed by the agar dilution method.Results: It was observed that 40.8% of the genotypes were cagA-positive; 66.9% were vacA s1m1 genotype and the iceA1 allele was found in 40.8%. A significant correlation (p = 0.0000) was observed between cagA positivity and vacA s1m1/iceA1 genotypes. Triple virulent genotypes were statistically associated with peptic ulcer (PU) (p = 0.0001) and Cla resistance (p = 0.0000). RAPD fingerprints obtained with AO2 primers identified clusters that were strongly associated with PU, virulence markers, and resistance to Cla and Mtz.Conclusions: The H. pylori isolates that harbored two or three virulence markers were more resistant to Cla and Mtz. Combined analysis of virulent genotypes and resistance patterns may permit identification of high-risk patients to prevent PU later in life or to avoid antimicrobial treatment failure.Fil: Vega, Alba Edith. Universidad Nacional de San Luis; ArgentinaFil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis; ArgentinaFil: Centrobi, Olga Nélida. Universidad Nacional de San Luis; ArgentinaFil: Silva, Humberto de Jesus. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; ArgentinaElsevier2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13972Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de Jesus; Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina; Elsevier; International Journal Of Infectious Diseases : Ijid : Official Publication Of The International Society For Infectious Diseases.; 14s; 3; 5-2010; 85-921201-9712enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1201971210022782info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijid.2009.11.022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:11Zoai:ri.conicet.gov.ar:11336/13972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:11.92CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
title Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
spellingShingle Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
Vega, Alba Edith
Helicobacter
Antimicrobial
Virulence
Rapd-Pcr
title_short Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
title_full Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
title_fullStr Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
title_full_unstemmed Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
title_sort Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina
dc.creator.none.fl_str_mv Vega, Alba Edith
Cortiñas, Teresa Inés
Centrobi, Olga Nélida
Silva, Humberto de Jesus
author Vega, Alba Edith
author_facet Vega, Alba Edith
Cortiñas, Teresa Inés
Centrobi, Olga Nélida
Silva, Humberto de Jesus
author_role author
author2 Cortiñas, Teresa Inés
Centrobi, Olga Nélida
Silva, Humberto de Jesus
author2_role author
author
author
dc.subject.none.fl_str_mv Helicobacter
Antimicrobial
Virulence
Rapd-Pcr
topic Helicobacter
Antimicrobial
Virulence
Rapd-Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: To characterize Helicobacter pylori isolates from western Argentina using virulence markers and antimicrobial susceptibility patterns in order to assess the association between virulent genotypes, antimicrobial resistance, and disease. DNA fingerprinting was also evaluated for the segregation of virulent or resistant strain clusters. Methods: Genotyping of 299 H. pylori isolates was performed by PCR using specific primers for the cagA,vacA and iceA genes. Random amplification of polymorphic DNA (RAPD)-PCR and rep-PCR genetic clustering were assessed using five random primers and BOXA1R and ERIC primers, respectively. Resistance to clarithromycin (Cla) and metronidazole (Mtz) was assessed by the agar dilution method.Results: It was observed that 40.8% of the genotypes were cagA-positive; 66.9% were vacA s1m1 genotype and the iceA1 allele was found in 40.8%. A significant correlation (p = 0.0000) was observed between cagA positivity and vacA s1m1/iceA1 genotypes. Triple virulent genotypes were statistically associated with peptic ulcer (PU) (p = 0.0001) and Cla resistance (p = 0.0000). RAPD fingerprints obtained with AO2 primers identified clusters that were strongly associated with PU, virulence markers, and resistance to Cla and Mtz.Conclusions: The H. pylori isolates that harbored two or three virulence markers were more resistant to Cla and Mtz. Combined analysis of virulent genotypes and resistance patterns may permit identification of high-risk patients to prevent PU later in life or to avoid antimicrobial treatment failure.
Fil: Vega, Alba Edith. Universidad Nacional de San Luis; Argentina
Fil: Cortiñas, Teresa Inés. Universidad Nacional de San Luis; Argentina
Fil: Centrobi, Olga Nélida. Universidad Nacional de San Luis; Argentina
Fil: Silva, Humberto de Jesus. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis; Argentina
description Objective: To characterize Helicobacter pylori isolates from western Argentina using virulence markers and antimicrobial susceptibility patterns in order to assess the association between virulent genotypes, antimicrobial resistance, and disease. DNA fingerprinting was also evaluated for the segregation of virulent or resistant strain clusters. Methods: Genotyping of 299 H. pylori isolates was performed by PCR using specific primers for the cagA,vacA and iceA genes. Random amplification of polymorphic DNA (RAPD)-PCR and rep-PCR genetic clustering were assessed using five random primers and BOXA1R and ERIC primers, respectively. Resistance to clarithromycin (Cla) and metronidazole (Mtz) was assessed by the agar dilution method.Results: It was observed that 40.8% of the genotypes were cagA-positive; 66.9% were vacA s1m1 genotype and the iceA1 allele was found in 40.8%. A significant correlation (p = 0.0000) was observed between cagA positivity and vacA s1m1/iceA1 genotypes. Triple virulent genotypes were statistically associated with peptic ulcer (PU) (p = 0.0001) and Cla resistance (p = 0.0000). RAPD fingerprints obtained with AO2 primers identified clusters that were strongly associated with PU, virulence markers, and resistance to Cla and Mtz.Conclusions: The H. pylori isolates that harbored two or three virulence markers were more resistant to Cla and Mtz. Combined analysis of virulent genotypes and resistance patterns may permit identification of high-risk patients to prevent PU later in life or to avoid antimicrobial treatment failure.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13972
Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de Jesus; Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina; Elsevier; International Journal Of Infectious Diseases : Ijid : Official Publication Of The International Society For Infectious Diseases.; 14s; 3; 5-2010; 85-92
1201-9712
url http://hdl.handle.net/11336/13972
identifier_str_mv Vega, Alba Edith; Cortiñas, Teresa Inés; Centrobi, Olga Nélida; Silva, Humberto de Jesus; Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina; Elsevier; International Journal Of Infectious Diseases : Ijid : Official Publication Of The International Society For Infectious Diseases.; 14s; 3; 5-2010; 85-92
1201-9712
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1201971210022782
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijid.2009.11.022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613574988660736
score 13.070432

Publicaciones similares