Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats

Autores
Mastaglia, Silvina Rosana; Pellegrini, Gretel Gisela; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol; Friedman, Silvia María; Zeni, Susana Noemi
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+ D). They were then assigned during an additional 45 days to receive either + D or a diet lacking vit D (- D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in - D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either + D or - D according to experiment 1 and were treated with either placebo or 16 μg olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in - D/OPD than in + D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, + D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than - D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.
Fil: Mastaglia, Silvina Rosana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Gonzales Chaves, Macarena Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Materia
BISPHOSPHONATES
BONE MARKERS
OSTEOPENIA
RATS
VITAMIN D INSUFFICIENCY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117687
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117687
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in ratsMastaglia, Silvina RosanaPellegrini, Gretel GiselaMandalunis, Patricia MónicaGonzales Chaves, Macarena Maria SolFriedman, Silvia MaríaZeni, Susana NoemiBISPHOSPHONATESBONE MARKERSOSTEOPENIARATSVITAMIN D INSUFFICIENCYhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+ D). They were then assigned during an additional 45 days to receive either + D or a diet lacking vit D (- D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in - D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either + D or - D according to experiment 1 and were treated with either placebo or 16 μg olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in - D/OPD than in + D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, + D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than - D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.Fil: Mastaglia, Silvina Rosana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Gonzales Chaves, Macarena Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaElsevier Science Inc2006-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117687Mastaglia, Silvina Rosana; Pellegrini, Gretel Gisela; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol; Friedman, Silvia María; et al.; Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats; Elsevier Science Inc; Bone; 39; 4; 10-2006; 837-8448756-3282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S8756328206004200info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2006.04.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:20Zoai:ri.conicet.gov.ar:11336/117687instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:20.441CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
title Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
spellingShingle Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
Mastaglia, Silvina Rosana
BISPHOSPHONATES
BONE MARKERS
OSTEOPENIA
RATS
VITAMIN D INSUFFICIENCY
title_short Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
title_full Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
title_fullStr Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
title_full_unstemmed Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
title_sort Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats
dc.creator.none.fl_str_mv Mastaglia, Silvina Rosana
Pellegrini, Gretel Gisela
Mandalunis, Patricia Mónica
Gonzales Chaves, Macarena Maria Sol
Friedman, Silvia María
Zeni, Susana Noemi
author Mastaglia, Silvina Rosana
author_facet Mastaglia, Silvina Rosana
Pellegrini, Gretel Gisela
Mandalunis, Patricia Mónica
Gonzales Chaves, Macarena Maria Sol
Friedman, Silvia María
Zeni, Susana Noemi
author_role author
author2 Pellegrini, Gretel Gisela
Mandalunis, Patricia Mónica
Gonzales Chaves, Macarena Maria Sol
Friedman, Silvia María
Zeni, Susana Noemi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BISPHOSPHONATES
BONE MARKERS
OSTEOPENIA
RATS
VITAMIN D INSUFFICIENCY
topic BISPHOSPHONATES
BONE MARKERS
OSTEOPENIA
RATS
VITAMIN D INSUFFICIENCY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+ D). They were then assigned during an additional 45 days to receive either + D or a diet lacking vit D (- D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in - D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either + D or - D according to experiment 1 and were treated with either placebo or 16 μg olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in - D/OPD than in + D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, + D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than - D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.
Fil: Mastaglia, Silvina Rosana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Pellegrini, Gretel Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mandalunis, Patricia Mónica. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Gonzales Chaves, Macarena Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Friedman, Silvia María. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
description The present study was carried out to obtain an experimental model of vitamin D (vit D) insufficiency and established osteopenia (experiment 1) to then investigate whether vit D status, i.e. normal or insufficient, interferes with bone mass recovery resulting from bisphosphonate therapy (experiment 2). Rats (n = 40) underwent OVX (n = 32) or a sham operation (n = 8). The first 15 days post-surgery, all groups were kept under fluorescent tube lighting and fed a diet containing 200 IU% vit D (+ D). They were then assigned during an additional 45 days to receive either + D or a diet lacking vit D (- D) and kept under 12 h light/dark cycles using fluorescent or red lighting. Serum 25HOD was significantly lower in - D rats (P < 0.0001). The type of lighting did not induce differences in 25OHD, calcium (sCa), phosphorus (sP), bone alkaline phosphatase (b-AL), CTX, bone density or histology. No osteoid was observed in undecalcified bone sections. Experiment 2 (105 days): rats were fed either + D or - D according to experiment 1 and were treated with either placebo or 16 μg olpadronate (OPD)/100 g rat/week during the last 45 days. Whereas 25HOD was significantly lower (P < 0.0001) in - D/OPD than in + D/OPD rats, no significant differences in sCa, sP, b-AL or CTX were observed. OPD prevented the loss of lumbar spine (LS) and proximal tibia (PT) BMD and the decrease in bone volume (BV/TV) (P < 0.05) and in the number of trabeculae observed in untreated rats. However, + D/OPD animals presented significantly higher values of LS BMD, PT BMD and BV/TV than - D/OPD rats (P < 0.05). No osteoid was observed in undecalcified sections of bone. In summary, this is the first experimental study to provide evidence that differences in vit D status may affect the anticatabolic response to bisphosphonate treatment. However, the molecular mechanism through which vit D insufficiency reduces the effect of the aminobisphosphonate remains to be defined.
publishDate 2006
dc.date.none.fl_str_mv 2006-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117687
Mastaglia, Silvina Rosana; Pellegrini, Gretel Gisela; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol; Friedman, Silvia María; et al.; Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats; Elsevier Science Inc; Bone; 39; 4; 10-2006; 837-844
8756-3282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117687
identifier_str_mv Mastaglia, Silvina Rosana; Pellegrini, Gretel Gisela; Mandalunis, Patricia Mónica; Gonzales Chaves, Macarena Maria Sol; Friedman, Silvia María; et al.; Vitamin D insufficiency reduces the protective effect of bisphosphonate on ovariectomy-induced bone loss in rats; Elsevier Science Inc; Bone; 39; 4; 10-2006; 837-844
8756-3282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S8756328206004200
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2006.04.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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