Cultures of marine microalgae: a potential source of natural antioxidants

Autores
Bauchi, Ana Victoria; Sequeira, María Alejandra; Faraoni, María Belén; Damiani, Maria Cecilia; Popovich, Cecilia Angelines
Año de publicación
2024
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Marine diatoms have garnered significant attention as a potential source of natural antioxidants including fucoxanthin (Fx). In particular, numerous studies have shown that Fx has promising applications in human health, indicating its potential in pharmaceutical and nutraceutical industries. A significant Fx production by the marine diatom Halamphora coffeaeformis, isolated from Bahía Blanca Estuary (Argentina), was evidenced in our previous studies. We also verified that cultures of this species adapted at 100 µE m-2 s-1 of light intensity showed remarkable antioxidant activity. Under this scenario, the purpose of this study was to evaluate variations in the enhancement of antioxidant activity of extracts from H. coffeaformis cultures depending on different light condition adaptation. For this, an optimized protocol for 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical (ABTS?+) bleaching test was implemented. The diatom was cultured in f/2 medium at 60 μE m-2 s-1 of light intensity under a photoperiod of 12 h light:12 h dark. Fx-enriched extract was obtained by ethanolic treatment of H. coffeaformis biomass and its antioxidant activity was spectrophotometrically achieved by ABTS?+ scavenging activity measurements. The results showed an antioxidant activity of 47% for Fx-enriched extract at a concentration of 0.02 mg mL-1 and it represented half of that obtained by ascorbic acid (93%), used as a reference positive control. In addition, it was evidenced lower activity for concentrations between 0.005 and 0.04 mg mL-1 compared to our previous studies with H. coffeaeformis cultures conditioned at 100 µE m-2 s-1. In conclusion, this study demonstrates that pigment production in H. coffeaeformis can be improved by optimizing light intensity conditions and it also underscores the potential of this native species as a promising alternative source of natural antioxidants.
Fil: Bauchi, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Sequeira, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Faraoni, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; Argentina
LVI Reunión Anual de la Asociación Argentina de Farmacología Experimental
Bahía Blanca
Argentina
Asociación Argentina de Farmacología Experimental
Materia
ANTIOXIDANTS
HALAMPHORA COFFEAEFORMIS
PHARMACEUTICAL AND NUTRACEUTICAL
ANTIOXIDANT ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279544
Acceder
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spelling Cultures of marine microalgae: a potential source of natural antioxidantsBauchi, Ana VictoriaSequeira, María AlejandraFaraoni, María BelénDamiani, Maria CeciliaPopovich, Cecilia AngelinesANTIOXIDANTSHALAMPHORA COFFEAEFORMISPHARMACEUTICAL AND NUTRACEUTICALANTIOXIDANT ACTIVITYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Marine diatoms have garnered significant attention as a potential source of natural antioxidants including fucoxanthin (Fx). In particular, numerous studies have shown that Fx has promising applications in human health, indicating its potential in pharmaceutical and nutraceutical industries. A significant Fx production by the marine diatom Halamphora coffeaeformis, isolated from Bahía Blanca Estuary (Argentina), was evidenced in our previous studies. We also verified that cultures of this species adapted at 100 µE m-2 s-1 of light intensity showed remarkable antioxidant activity. Under this scenario, the purpose of this study was to evaluate variations in the enhancement of antioxidant activity of extracts from H. coffeaformis cultures depending on different light condition adaptation. For this, an optimized protocol for 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical (ABTS?+) bleaching test was implemented. The diatom was cultured in f/2 medium at 60 μE m-2 s-1 of light intensity under a photoperiod of 12 h light:12 h dark. Fx-enriched extract was obtained by ethanolic treatment of H. coffeaformis biomass and its antioxidant activity was spectrophotometrically achieved by ABTS?+ scavenging activity measurements. The results showed an antioxidant activity of 47% for Fx-enriched extract at a concentration of 0.02 mg mL-1 and it represented half of that obtained by ascorbic acid (93%), used as a reference positive control. In addition, it was evidenced lower activity for concentrations between 0.005 and 0.04 mg mL-1 compared to our previous studies with H. coffeaeformis cultures conditioned at 100 µE m-2 s-1. In conclusion, this study demonstrates that pigment production in H. coffeaeformis can be improved by optimizing light intensity conditions and it also underscores the potential of this native species as a promising alternative source of natural antioxidants.Fil: Bauchi, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Sequeira, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Faraoni, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; ArgentinaLVI Reunión Anual de la Asociación Argentina de Farmacología ExperimentalBahía BlancaArgentinaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Farmacología Experimental2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279544Cultures of marine microalgae: a potential source of natural antioxidants; LVI Reunión Anual de la Asociación Argentina de Farmacología Experimental; Bahía Blanca; Argentina; 2024; 121-121978-631-90806-0-5CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/wp-content/uploads/2024/11/ABSTRACT-BOOK-AAFE-2024_Final.pdfinfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:02:25Zoai:ri.conicet.gov.ar:11336/279544instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:02:25.521CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cultures of marine microalgae: a potential source of natural antioxidants
title Cultures of marine microalgae: a potential source of natural antioxidants
spellingShingle Cultures of marine microalgae: a potential source of natural antioxidants
Bauchi, Ana Victoria
ANTIOXIDANTS
HALAMPHORA COFFEAEFORMIS
PHARMACEUTICAL AND NUTRACEUTICAL
ANTIOXIDANT ACTIVITY
title_short Cultures of marine microalgae: a potential source of natural antioxidants
title_full Cultures of marine microalgae: a potential source of natural antioxidants
title_fullStr Cultures of marine microalgae: a potential source of natural antioxidants
title_full_unstemmed Cultures of marine microalgae: a potential source of natural antioxidants
title_sort Cultures of marine microalgae: a potential source of natural antioxidants
dc.creator.none.fl_str_mv Bauchi, Ana Victoria
Sequeira, María Alejandra
Faraoni, María Belén
Damiani, Maria Cecilia
Popovich, Cecilia Angelines
author Bauchi, Ana Victoria
author_facet Bauchi, Ana Victoria
Sequeira, María Alejandra
Faraoni, María Belén
Damiani, Maria Cecilia
Popovich, Cecilia Angelines
author_role author
author2 Sequeira, María Alejandra
Faraoni, María Belén
Damiani, Maria Cecilia
Popovich, Cecilia Angelines
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANTS
HALAMPHORA COFFEAEFORMIS
PHARMACEUTICAL AND NUTRACEUTICAL
ANTIOXIDANT ACTIVITY
topic ANTIOXIDANTS
HALAMPHORA COFFEAEFORMIS
PHARMACEUTICAL AND NUTRACEUTICAL
ANTIOXIDANT ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Marine diatoms have garnered significant attention as a potential source of natural antioxidants including fucoxanthin (Fx). In particular, numerous studies have shown that Fx has promising applications in human health, indicating its potential in pharmaceutical and nutraceutical industries. A significant Fx production by the marine diatom Halamphora coffeaeformis, isolated from Bahía Blanca Estuary (Argentina), was evidenced in our previous studies. We also verified that cultures of this species adapted at 100 µE m-2 s-1 of light intensity showed remarkable antioxidant activity. Under this scenario, the purpose of this study was to evaluate variations in the enhancement of antioxidant activity of extracts from H. coffeaformis cultures depending on different light condition adaptation. For this, an optimized protocol for 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical (ABTS?+) bleaching test was implemented. The diatom was cultured in f/2 medium at 60 μE m-2 s-1 of light intensity under a photoperiod of 12 h light:12 h dark. Fx-enriched extract was obtained by ethanolic treatment of H. coffeaformis biomass and its antioxidant activity was spectrophotometrically achieved by ABTS?+ scavenging activity measurements. The results showed an antioxidant activity of 47% for Fx-enriched extract at a concentration of 0.02 mg mL-1 and it represented half of that obtained by ascorbic acid (93%), used as a reference positive control. In addition, it was evidenced lower activity for concentrations between 0.005 and 0.04 mg mL-1 compared to our previous studies with H. coffeaeformis cultures conditioned at 100 µE m-2 s-1. In conclusion, this study demonstrates that pigment production in H. coffeaeformis can be improved by optimizing light intensity conditions and it also underscores the potential of this native species as a promising alternative source of natural antioxidants.
Fil: Bauchi, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Sequeira, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Faraoni, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Provincia de Buenos Aires. Dirección General de Cultura y Educación. Universidad Provincial del Sudoeste. Centro de Emprendedorismo y Desarrollo Territorial Sustentable; Argentina
LVI Reunión Anual de la Asociación Argentina de Farmacología Experimental
Bahía Blanca
Argentina
Asociación Argentina de Farmacología Experimental
description Marine diatoms have garnered significant attention as a potential source of natural antioxidants including fucoxanthin (Fx). In particular, numerous studies have shown that Fx has promising applications in human health, indicating its potential in pharmaceutical and nutraceutical industries. A significant Fx production by the marine diatom Halamphora coffeaeformis, isolated from Bahía Blanca Estuary (Argentina), was evidenced in our previous studies. We also verified that cultures of this species adapted at 100 µE m-2 s-1 of light intensity showed remarkable antioxidant activity. Under this scenario, the purpose of this study was to evaluate variations in the enhancement of antioxidant activity of extracts from H. coffeaformis cultures depending on different light condition adaptation. For this, an optimized protocol for 2,2?-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical (ABTS?+) bleaching test was implemented. The diatom was cultured in f/2 medium at 60 μE m-2 s-1 of light intensity under a photoperiod of 12 h light:12 h dark. Fx-enriched extract was obtained by ethanolic treatment of H. coffeaformis biomass and its antioxidant activity was spectrophotometrically achieved by ABTS?+ scavenging activity measurements. The results showed an antioxidant activity of 47% for Fx-enriched extract at a concentration of 0.02 mg mL-1 and it represented half of that obtained by ascorbic acid (93%), used as a reference positive control. In addition, it was evidenced lower activity for concentrations between 0.005 and 0.04 mg mL-1 compared to our previous studies with H. coffeaeformis cultures conditioned at 100 µE m-2 s-1. In conclusion, this study demonstrates that pigment production in H. coffeaeformis can be improved by optimizing light intensity conditions and it also underscores the potential of this native species as a promising alternative source of natural antioxidants.
publishDate 2024
dc.date.none.fl_str_mv 2024
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Cultures of marine microalgae: a potential source of natural antioxidants; LVI Reunión Anual de la Asociación Argentina de Farmacología Experimental; Bahía Blanca; Argentina; 2024; 121-121
978-631-90806-0-5
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279544
identifier_str_mv Cultures of marine microalgae: a potential source of natural antioxidants; LVI Reunión Anual de la Asociación Argentina de Farmacología Experimental; Bahía Blanca; Argentina; 2024; 121-121
978-631-90806-0-5
CONICET Digital
CONICET
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