Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana

Autores
Mohamed, Ana Mariel; Salinas, A.; Mattana, C.; Satorres, S.; Ortega, C.; Favier, L.; Cifuente, Diego Alberto
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work we investigate the changes in the antibacterial activity and the antioxidant properties of modified extracts of Zinnia peruviana (L.) L. campesterol, stigmasterol and β-sitosterol were identified as majortriterpenoids in the natural extract, by CG-EIMS and NIST Mass Spectrometry Data Center. The acetonic extract was chemically modified by reactions of acid hydrolysis, sulfonylation, acetylation, methylation and silylation. The in-vitro antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria. The acetonic extract showed significant activity only against Gram-positive bacteria (MIC/MBC = 2/9 mg/mL). The hydrolyzed extract improve the bioactivity to both, Gram-positive and Gram-negative (MIC/MBC = 2/8 mg/mL). Selectively, the acetylated extract was only effective toward Staphylococcus (MIC/MBC = 7/7 mg/mL). The tosylated extract was active against Gram-positive and P. aeruginosa (MIC/MBC = 24/24 mg/mL) and the silylated against Grampositiveand E. coli. (MIC/MBC = 15/15 mg/mL). The antioxidant activity was determining by DPPH and ABTS assays. Hydrolyzed extract increased the antioxidant properties (DPPH, IC50,(mg/mL) = 0.077 ± 0.007; ABTS IC50,(mg/mL) = 0.032 ± 0.005). The acylated and alkylated extracts reduce the bioactivity and silylated derivative eliminated the bioactivity. These results could be considered as a possible strategy to obtain antimicrobial agents from herbal medicines.
Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mattana, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Satorres, S.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Ortega, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Favier, L.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Materia
ZINNIA PERUVIANA
MODIFIED EXTRACTS
PHYTOSTEROLS
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT PROPERTIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69623
Acceder
id CONICETDig_dd45ae48a1201ed38ece7f27b60ea7e3
oai_identifier_str oai:ri.conicet.gov.ar:11336/69623
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruvianaMohamed, Ana MarielSalinas, A.Mattana, C.Satorres, S.Ortega, C.Favier, L.Cifuente, Diego AlbertoZINNIA PERUVIANAMODIFIED EXTRACTSPHYTOSTEROLSANTIBACTERIAL ACTIVITYANTIOXIDANT PROPERTIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present work we investigate the changes in the antibacterial activity and the antioxidant properties of modified extracts of Zinnia peruviana (L.) L. campesterol, stigmasterol and β-sitosterol were identified as majortriterpenoids in the natural extract, by CG-EIMS and NIST Mass Spectrometry Data Center. The acetonic extract was chemically modified by reactions of acid hydrolysis, sulfonylation, acetylation, methylation and silylation. The in-vitro antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria. The acetonic extract showed significant activity only against Gram-positive bacteria (MIC/MBC = 2/9 mg/mL). The hydrolyzed extract improve the bioactivity to both, Gram-positive and Gram-negative (MIC/MBC = 2/8 mg/mL). Selectively, the acetylated extract was only effective toward Staphylococcus (MIC/MBC = 7/7 mg/mL). The tosylated extract was active against Gram-positive and P. aeruginosa (MIC/MBC = 24/24 mg/mL) and the silylated against Grampositiveand E. coli. (MIC/MBC = 15/15 mg/mL). The antioxidant activity was determining by DPPH and ABTS assays. Hydrolyzed extract increased the antioxidant properties (DPPH, IC50,(mg/mL) = 0.077 ± 0.007; ABTS IC50,(mg/mL) = 0.032 ± 0.005). The acylated and alkylated extracts reduce the bioactivity and silylated derivative eliminated the bioactivity. These results could be considered as a possible strategy to obtain antimicrobial agents from herbal medicines.Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Mattana, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Satorres, S.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Ortega, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Favier, L.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaSocietà Italo-Latinoamericana di Etnomedicina2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69623Mohamed, Ana Mariel; Salinas, A.; Mattana, C.; Satorres, S.; Ortega, C.; et al.; Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana; Società Italo-Latinoamericana di Etnomedicina; PharmacologyOnLine; 2; 8-2017; 162-1681827-8620CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/files/archives/2017/vol2/PhOL_2017_2_A015_Mohamed_162_168.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:54Zoai:ri.conicet.gov.ar:11336/69623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:54.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
title Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
spellingShingle Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
Mohamed, Ana Mariel
ZINNIA PERUVIANA
MODIFIED EXTRACTS
PHYTOSTEROLS
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT PROPERTIES
title_short Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
title_full Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
title_fullStr Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
title_full_unstemmed Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
title_sort Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana
dc.creator.none.fl_str_mv Mohamed, Ana Mariel
Salinas, A.
Mattana, C.
Satorres, S.
Ortega, C.
Favier, L.
Cifuente, Diego Alberto
author Mohamed, Ana Mariel
author_facet Mohamed, Ana Mariel
Salinas, A.
Mattana, C.
Satorres, S.
Ortega, C.
Favier, L.
Cifuente, Diego Alberto
author_role author
author2 Salinas, A.
Mattana, C.
Satorres, S.
Ortega, C.
Favier, L.
Cifuente, Diego Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ZINNIA PERUVIANA
MODIFIED EXTRACTS
PHYTOSTEROLS
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT PROPERTIES
topic ZINNIA PERUVIANA
MODIFIED EXTRACTS
PHYTOSTEROLS
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT PROPERTIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work we investigate the changes in the antibacterial activity and the antioxidant properties of modified extracts of Zinnia peruviana (L.) L. campesterol, stigmasterol and β-sitosterol were identified as majortriterpenoids in the natural extract, by CG-EIMS and NIST Mass Spectrometry Data Center. The acetonic extract was chemically modified by reactions of acid hydrolysis, sulfonylation, acetylation, methylation and silylation. The in-vitro antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria. The acetonic extract showed significant activity only against Gram-positive bacteria (MIC/MBC = 2/9 mg/mL). The hydrolyzed extract improve the bioactivity to both, Gram-positive and Gram-negative (MIC/MBC = 2/8 mg/mL). Selectively, the acetylated extract was only effective toward Staphylococcus (MIC/MBC = 7/7 mg/mL). The tosylated extract was active against Gram-positive and P. aeruginosa (MIC/MBC = 24/24 mg/mL) and the silylated against Grampositiveand E. coli. (MIC/MBC = 15/15 mg/mL). The antioxidant activity was determining by DPPH and ABTS assays. Hydrolyzed extract increased the antioxidant properties (DPPH, IC50,(mg/mL) = 0.077 ± 0.007; ABTS IC50,(mg/mL) = 0.032 ± 0.005). The acylated and alkylated extracts reduce the bioactivity and silylated derivative eliminated the bioactivity. These results could be considered as a possible strategy to obtain antimicrobial agents from herbal medicines.
Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Mattana, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Satorres, S.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Ortega, C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Favier, L.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
description In the present work we investigate the changes in the antibacterial activity and the antioxidant properties of modified extracts of Zinnia peruviana (L.) L. campesterol, stigmasterol and β-sitosterol were identified as majortriterpenoids in the natural extract, by CG-EIMS and NIST Mass Spectrometry Data Center. The acetonic extract was chemically modified by reactions of acid hydrolysis, sulfonylation, acetylation, methylation and silylation. The in-vitro antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria. The acetonic extract showed significant activity only against Gram-positive bacteria (MIC/MBC = 2/9 mg/mL). The hydrolyzed extract improve the bioactivity to both, Gram-positive and Gram-negative (MIC/MBC = 2/8 mg/mL). Selectively, the acetylated extract was only effective toward Staphylococcus (MIC/MBC = 7/7 mg/mL). The tosylated extract was active against Gram-positive and P. aeruginosa (MIC/MBC = 24/24 mg/mL) and the silylated against Grampositiveand E. coli. (MIC/MBC = 15/15 mg/mL). The antioxidant activity was determining by DPPH and ABTS assays. Hydrolyzed extract increased the antioxidant properties (DPPH, IC50,(mg/mL) = 0.077 ± 0.007; ABTS IC50,(mg/mL) = 0.032 ± 0.005). The acylated and alkylated extracts reduce the bioactivity and silylated derivative eliminated the bioactivity. These results could be considered as a possible strategy to obtain antimicrobial agents from herbal medicines.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69623
Mohamed, Ana Mariel; Salinas, A.; Mattana, C.; Satorres, S.; Ortega, C.; et al.; Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana; Società Italo-Latinoamericana di Etnomedicina; PharmacologyOnLine; 2; 8-2017; 162-168
1827-8620
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69623
identifier_str_mv Mohamed, Ana Mariel; Salinas, A.; Mattana, C.; Satorres, S.; Ortega, C.; et al.; Improving in the antibacterial activity and the antioxidant properties of chemically modified extracts of Zinnia peruviana; Società Italo-Latinoamericana di Etnomedicina; PharmacologyOnLine; 2; 8-2017; 162-168
1827-8620
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/files/archives/2017/vol2/PhOL_2017_2_A015_Mohamed_162_168.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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