Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
- Autores
- Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.
Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina - Materia
-
ALOYSIA POLYSTACHYA
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT ACTIVITY
FLAVONOIDS
LIPPIA TURBINATA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/104775
Ver los metadatos del registro completo
id |
CONICETDig_e94900c9c07145404eeb9e41f4838867 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/104775 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)Aguado, María InésDudik, NéstorPérez Zamora, Cristina MariselTorres, Carola AnalíaNuñez, María BeatrizALOYSIA POLYSTACHYAANTIBACTERIAL ACTIVITYANTIOXIDANT ACTIVITYFLAVONOIDSLIPPIA TURBINATAhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaInnovare Academic Sciences2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104775Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-3952656-00970975-1491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://innovareacademics.in/journals/index.php/ijpps/article/view/9856info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:28Zoai:ri.conicet.gov.ar:11336/104775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:28.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
title |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
spellingShingle |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) Aguado, María Inés ALOYSIA POLYSTACHYA ANTIBACTERIAL ACTIVITY ANTIOXIDANT ACTIVITY FLAVONOIDS LIPPIA TURBINATA |
title_short |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
title_full |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
title_fullStr |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
title_full_unstemmed |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
title_sort |
Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae) |
dc.creator.none.fl_str_mv |
Aguado, María Inés Dudik, Néstor Pérez Zamora, Cristina Marisel Torres, Carola Analía Nuñez, María Beatriz |
author |
Aguado, María Inés |
author_facet |
Aguado, María Inés Dudik, Néstor Pérez Zamora, Cristina Marisel Torres, Carola Analía Nuñez, María Beatriz |
author_role |
author |
author2 |
Dudik, Néstor Pérez Zamora, Cristina Marisel Torres, Carola Analía Nuñez, María Beatriz |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ALOYSIA POLYSTACHYA ANTIBACTERIAL ACTIVITY ANTIOXIDANT ACTIVITY FLAVONOIDS LIPPIA TURBINATA |
topic |
ALOYSIA POLYSTACHYA ANTIBACTERIAL ACTIVITY ANTIOXIDANT ACTIVITY FLAVONOIDS LIPPIA TURBINATA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.7 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities. Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina Fil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina Fil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina |
description |
Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/104775 Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-395 2656-0097 0975-1491 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/104775 |
identifier_str_mv |
Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-395 2656-0097 0975-1491 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://innovareacademics.in/journals/index.php/ijpps/article/view/9856 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Innovare Academic Sciences |
publisher.none.fl_str_mv |
Innovare Academic Sciences |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614457540476928 |
score |
13.070432 |