Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)

Autores
Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.
Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Materia
ALOYSIA POLYSTACHYA
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT ACTIVITY
FLAVONOIDS
LIPPIA TURBINATA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104775

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network_name_str CONICET Digital (CONICET)
spelling Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)Aguado, María InésDudik, NéstorPérez Zamora, Cristina MariselTorres, Carola AnalíaNuñez, María BeatrizALOYSIA POLYSTACHYAANTIBACTERIAL ACTIVITYANTIOXIDANT ACTIVITYFLAVONOIDSLIPPIA TURBINATAhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaInnovare Academic Sciences2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104775Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-3952656-00970975-1491CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://innovareacademics.in/journals/index.php/ijpps/article/view/9856info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:28Zoai:ri.conicet.gov.ar:11336/104775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:28.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
title Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
spellingShingle Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
Aguado, María Inés
ALOYSIA POLYSTACHYA
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT ACTIVITY
FLAVONOIDS
LIPPIA TURBINATA
title_short Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
title_full Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
title_fullStr Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
title_full_unstemmed Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
title_sort Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae)
dc.creator.none.fl_str_mv Aguado, María Inés
Dudik, Néstor
Pérez Zamora, Cristina Marisel
Torres, Carola Analía
Nuñez, María Beatriz
author Aguado, María Inés
author_facet Aguado, María Inés
Dudik, Néstor
Pérez Zamora, Cristina Marisel
Torres, Carola Analía
Nuñez, María Beatriz
author_role author
author2 Dudik, Néstor
Pérez Zamora, Cristina Marisel
Torres, Carola Analía
Nuñez, María Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALOYSIA POLYSTACHYA
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT ACTIVITY
FLAVONOIDS
LIPPIA TURBINATA
topic ALOYSIA POLYSTACHYA
ANTIBACTERIAL ACTIVITY
ANTIOXIDANT ACTIVITY
FLAVONOIDS
LIPPIA TURBINATA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.
Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Dudik, Néstor. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Pérez Zamora, Cristina Marisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
description Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterialactivities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled toelectrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using FolinCiocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterialactivity was determined by theantibacterial synergism.Results: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented thehighest total phenolic content and antioxidant activity.employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect againstmethicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in therespective crude extracts. However, it could be considered that the found flavonoids play an important roleagar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possibleExtracts from both species would have bactericidal activity against Staphylococcus strainsin the development of these activities.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104775
Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-395
2656-0097
0975-1491
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104775
identifier_str_mv Aguado, María Inés; Dudik, Néstor; Pérez Zamora, Cristina Marisel; Torres, Carola Analía; Nuñez, María Beatriz; Antioxidant and antibacterial activities of hydroalcoholic extracts from aloysia polystachya griseb moldenke and lippia turbinata griseb (Verbenaceae); Innovare Academic Sciences; International Journal of Pharmacy and Pharmaceutical Sciences; 8; 3; 3-2016; 393-395
2656-0097
0975-1491
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://innovareacademics.in/journals/index.php/ijpps/article/view/9856
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Innovare Academic Sciences
publisher.none.fl_str_mv Innovare Academic Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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