Control of citrus pathogens by protein extracts from Solanum tuberosum tubers

Autores
Rodriguez, Ivana Fabiola; Sayago, Jorge E.; Torres, Sebastián; Zampini, Iris Catiana; Isla, Maria Ines; Ordóñez, Roxana Mabel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A national program for citrus certification was started in Argentina in 2005 in order to provide healthy fruits free of toxic residues. In line with this goal, the aim of this study was to evaluate the efficacy of natural products, protein extracts obtained from potato tubers for the control of fungi responsible for disease in post-harvest citrus fruits. Different protein fractions were obtained from Solanum tuberosum tubers (IF25, IF50, SF25 and SF50) and their effect were evaluated on Penicillium digitatum and Geotrichum candidum, two citrus-pathogenic fungi. All fractions showed antifungal activity against both fungi species, the intensity of this activity being dependent on the type of fungus and extract. The fraction IF25 was the most active as an antifungal agent: it inhibited the mycelia growth of both pathogens, the elongation of the germ tube ofP. digitatum and the conidial isotropic growth of G. candidum as well as its polygalacturonase activity. None of the IF25 concentrations were mutagenic in Salmonella typhimurium TA98 or TA100 strains. The efficacy of protein extracts to control P. digitatum and G. candidum growth was tested in artificially inoculated citrus fruits. Extracts were tested built-in to the wax used in citrus industry to coat and protect the fruit. The IF25 extract was effective in inhibiting the growth and development of P.digitatum and G. candidum. Consequently, the IF25 extract plus the wax could be used preventively in controlling fungal infection on post-harvest citrus.
Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Sayago, Jorge E.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Torres, Sebastián. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Potato Protein Extracts
Antifungal Activity
Citrus Fruit
Penicillium Digitatum
Geotrichum Candidum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6801
Acceder
id CONICETDig_daec7f06bfe005fd18c70e8b7b952452
oai_identifier_str oai:ri.conicet.gov.ar:11336/6801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Control of citrus pathogens by protein extracts from Solanum tuberosum tubersRodriguez, Ivana FabiolaSayago, Jorge E.Torres, SebastiánZampini, Iris CatianaIsla, Maria InesOrdóñez, Roxana MabelPotato Protein ExtractsAntifungal ActivityCitrus FruitPenicillium DigitatumGeotrichum Candidumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A national program for citrus certification was started in Argentina in 2005 in order to provide healthy fruits free of toxic residues. In line with this goal, the aim of this study was to evaluate the efficacy of natural products, protein extracts obtained from potato tubers for the control of fungi responsible for disease in post-harvest citrus fruits. Different protein fractions were obtained from Solanum tuberosum tubers (IF25, IF50, SF25 and SF50) and their effect were evaluated on Penicillium digitatum and Geotrichum candidum, two citrus-pathogenic fungi. All fractions showed antifungal activity against both fungi species, the intensity of this activity being dependent on the type of fungus and extract. The fraction IF25 was the most active as an antifungal agent: it inhibited the mycelia growth of both pathogens, the elongation of the germ tube ofP. digitatum and the conidial isotropic growth of G. candidum as well as its polygalacturonase activity. None of the IF25 concentrations were mutagenic in Salmonella typhimurium TA98 or TA100 strains. The efficacy of protein extracts to control P. digitatum and G. candidum growth was tested in artificially inoculated citrus fruits. Extracts were tested built-in to the wax used in citrus industry to coat and protect the fruit. The IF25 extract was effective in inhibiting the growth and development of P.digitatum and G. candidum. Consequently, the IF25 extract plus the wax could be used preventively in controlling fungal infection on post-harvest citrus.Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; ArgentinaFil: Sayago, Jorge E.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Torres, Sebastián. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ordóñez, Roxana Mabel. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaSpringer2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6801Rodriguez, Ivana Fabiola; Sayago, Jorge E.; Torres, Sebastián; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Control of citrus pathogens by protein extracts from Solanum tuberosum tubers; Springer; European Journal of Plant Pathology; 141; 3; 3-2015; 585-5950929-1873enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-014-0566-7info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10658-014-0566-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:34Zoai:ri.conicet.gov.ar:11336/6801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:35.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
title Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
spellingShingle Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
Rodriguez, Ivana Fabiola
Potato Protein Extracts
Antifungal Activity
Citrus Fruit
Penicillium Digitatum
Geotrichum Candidum
title_short Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
title_full Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
title_fullStr Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
title_full_unstemmed Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
title_sort Control of citrus pathogens by protein extracts from Solanum tuberosum tubers
dc.creator.none.fl_str_mv Rodriguez, Ivana Fabiola
Sayago, Jorge E.
Torres, Sebastián
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author Rodriguez, Ivana Fabiola
author_facet Rodriguez, Ivana Fabiola
Sayago, Jorge E.
Torres, Sebastián
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author_role author
author2 Sayago, Jorge E.
Torres, Sebastián
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Potato Protein Extracts
Antifungal Activity
Citrus Fruit
Penicillium Digitatum
Geotrichum Candidum
topic Potato Protein Extracts
Antifungal Activity
Citrus Fruit
Penicillium Digitatum
Geotrichum Candidum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A national program for citrus certification was started in Argentina in 2005 in order to provide healthy fruits free of toxic residues. In line with this goal, the aim of this study was to evaluate the efficacy of natural products, protein extracts obtained from potato tubers for the control of fungi responsible for disease in post-harvest citrus fruits. Different protein fractions were obtained from Solanum tuberosum tubers (IF25, IF50, SF25 and SF50) and their effect were evaluated on Penicillium digitatum and Geotrichum candidum, two citrus-pathogenic fungi. All fractions showed antifungal activity against both fungi species, the intensity of this activity being dependent on the type of fungus and extract. The fraction IF25 was the most active as an antifungal agent: it inhibited the mycelia growth of both pathogens, the elongation of the germ tube ofP. digitatum and the conidial isotropic growth of G. candidum as well as its polygalacturonase activity. None of the IF25 concentrations were mutagenic in Salmonella typhimurium TA98 or TA100 strains. The efficacy of protein extracts to control P. digitatum and G. candidum growth was tested in artificially inoculated citrus fruits. Extracts were tested built-in to the wax used in citrus industry to coat and protect the fruit. The IF25 extract was effective in inhibiting the growth and development of P.digitatum and G. candidum. Consequently, the IF25 extract plus the wax could be used preventively in controlling fungal infection on post-harvest citrus.
Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina
Fil: Sayago, Jorge E.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Torres, Sebastián. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales E Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description A national program for citrus certification was started in Argentina in 2005 in order to provide healthy fruits free of toxic residues. In line with this goal, the aim of this study was to evaluate the efficacy of natural products, protein extracts obtained from potato tubers for the control of fungi responsible for disease in post-harvest citrus fruits. Different protein fractions were obtained from Solanum tuberosum tubers (IF25, IF50, SF25 and SF50) and their effect were evaluated on Penicillium digitatum and Geotrichum candidum, two citrus-pathogenic fungi. All fractions showed antifungal activity against both fungi species, the intensity of this activity being dependent on the type of fungus and extract. The fraction IF25 was the most active as an antifungal agent: it inhibited the mycelia growth of both pathogens, the elongation of the germ tube ofP. digitatum and the conidial isotropic growth of G. candidum as well as its polygalacturonase activity. None of the IF25 concentrations were mutagenic in Salmonella typhimurium TA98 or TA100 strains. The efficacy of protein extracts to control P. digitatum and G. candidum growth was tested in artificially inoculated citrus fruits. Extracts were tested built-in to the wax used in citrus industry to coat and protect the fruit. The IF25 extract was effective in inhibiting the growth and development of P.digitatum and G. candidum. Consequently, the IF25 extract plus the wax could be used preventively in controlling fungal infection on post-harvest citrus.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6801
Rodriguez, Ivana Fabiola; Sayago, Jorge E.; Torres, Sebastián; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Control of citrus pathogens by protein extracts from Solanum tuberosum tubers; Springer; European Journal of Plant Pathology; 141; 3; 3-2015; 585-595
0929-1873
url http://hdl.handle.net/11336/6801
identifier_str_mv Rodriguez, Ivana Fabiola; Sayago, Jorge E.; Torres, Sebastián; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Control of citrus pathogens by protein extracts from Solanum tuberosum tubers; Springer; European Journal of Plant Pathology; 141; 3; 3-2015; 585-595
0929-1873
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-014-0566-7
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10658-014-0566-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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