Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases

Autores
Baigorria, Carina Giselle; Cerioni, Luciana; Debes, Mario Alberto; Ledesma, Ana Estela; Alastuey, Patricio; Tirado, Monica Cecilia; Volentini, Sabrina Inès; Rapisarda, Viviana Andrea
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Postharvest fungal diseases are the main cause of economic losses in lemon production. The continued use of synthetic fungicides to control the diseases favors the emergence of resistant strains, which encourages the search for alternatives. The aim of this study was to assess the efficacy of metallic nanoparticles (NPs) as antifungal agents against local isolates of Penicillium digitatum and Penicillium italicum, each of them in a fungicide-sensitive and -resistant version, and a Geotrichum citri-aurantii isolate. NPs of ZnO, CuO, and Ag were synthesized and characterized by spectroscopy and microscopy, presenting average sizes < 25 nm and spherical shapes. ZnO-NPs did not present antifungal activity at the assayed conditions, while the minimum fungicidal concentrations (MFCs) were 1000 and 10 µg mL−1 for CuO-NPs and Ag-NPs, respectively. The NPs’ antimicrobial action included conidial membrane permeability and strong intracellular disorganization. Moreover, the Ag-NPs reduced green mold incidence on inoculated lemons when applied to the fruit. Taken together, Ag-NPs were effective in inhibiting both fungicide-sensitive and -resistant isolates of the main lemon postharvest pathogens, suggesting their potential use as an alternative approach.
Fil: Baigorria, Carina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Debes, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Ledesma, Ana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Alastuey, Patricio. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; Argentina
Fil: Tirado, Monica Cecilia. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; Argentina
Fil: Volentini, Sabrina Inès. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
Geotrichum citri-aurantii
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256462
Acceder
id CONICETDig_d6f0543d31efe9913e53c31caaa9ac6e
oai_identifier_str oai:ri.conicet.gov.ar:11336/256462
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon DiseasesBaigorria, Carina GiselleCerioni, LucianaDebes, Mario AlbertoLedesma, Ana EstelaAlastuey, PatricioTirado, Monica CeciliaVolentini, Sabrina InèsRapisarda, Viviana Andreasilver nanoparticlesPenicillium italicumcupric oxide nanoparticlesPenicillium digitatumGeotrichum citri-aurantiisilver nanoparticlesPenicillium italicumcupric oxide nanoparticlesPenicillium digitatumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Postharvest fungal diseases are the main cause of economic losses in lemon production. The continued use of synthetic fungicides to control the diseases favors the emergence of resistant strains, which encourages the search for alternatives. The aim of this study was to assess the efficacy of metallic nanoparticles (NPs) as antifungal agents against local isolates of Penicillium digitatum and Penicillium italicum, each of them in a fungicide-sensitive and -resistant version, and a Geotrichum citri-aurantii isolate. NPs of ZnO, CuO, and Ag were synthesized and characterized by spectroscopy and microscopy, presenting average sizes < 25 nm and spherical shapes. ZnO-NPs did not present antifungal activity at the assayed conditions, while the minimum fungicidal concentrations (MFCs) were 1000 and 10 µg mL−1 for CuO-NPs and Ag-NPs, respectively. The NPs’ antimicrobial action included conidial membrane permeability and strong intracellular disorganization. Moreover, the Ag-NPs reduced green mold incidence on inoculated lemons when applied to the fruit. Taken together, Ag-NPs were effective in inhibiting both fungicide-sensitive and -resistant isolates of the main lemon postharvest pathogens, suggesting their potential use as an alternative approach.Fil: Baigorria, Carina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Debes, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Ledesma, Ana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; ArgentinaFil: Alastuey, Patricio. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; ArgentinaFil: Tirado, Monica Cecilia. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; ArgentinaFil: Volentini, Sabrina Inès. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaMDPI2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256462Baigorria, Carina Giselle; Cerioni, Luciana; Debes, Mario Alberto; Ledesma, Ana Estela; Alastuey, Patricio; et al.; Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases; MDPI; Journal of Fungi; 10; 11; 11-2024; 1-152309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/10/11/782info:eu-repo/semantics/altIdentifier/doi/10.3390/jof10110782info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:20Zoai:ri.conicet.gov.ar:11336/256462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:20.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
title Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
spellingShingle Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
Baigorria, Carina Giselle
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
Geotrichum citri-aurantii
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
title_short Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
title_full Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
title_fullStr Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
title_full_unstemmed Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
title_sort Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases
dc.creator.none.fl_str_mv Baigorria, Carina Giselle
Cerioni, Luciana
Debes, Mario Alberto
Ledesma, Ana Estela
Alastuey, Patricio
Tirado, Monica Cecilia
Volentini, Sabrina Inès
Rapisarda, Viviana Andrea
author Baigorria, Carina Giselle
author_facet Baigorria, Carina Giselle
Cerioni, Luciana
Debes, Mario Alberto
Ledesma, Ana Estela
Alastuey, Patricio
Tirado, Monica Cecilia
Volentini, Sabrina Inès
Rapisarda, Viviana Andrea
author_role author
author2 Cerioni, Luciana
Debes, Mario Alberto
Ledesma, Ana Estela
Alastuey, Patricio
Tirado, Monica Cecilia
Volentini, Sabrina Inès
Rapisarda, Viviana Andrea
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
Geotrichum citri-aurantii
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
topic silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
Geotrichum citri-aurantii
silver nanoparticles
Penicillium italicum
cupric oxide nanoparticles
Penicillium digitatum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Postharvest fungal diseases are the main cause of economic losses in lemon production. The continued use of synthetic fungicides to control the diseases favors the emergence of resistant strains, which encourages the search for alternatives. The aim of this study was to assess the efficacy of metallic nanoparticles (NPs) as antifungal agents against local isolates of Penicillium digitatum and Penicillium italicum, each of them in a fungicide-sensitive and -resistant version, and a Geotrichum citri-aurantii isolate. NPs of ZnO, CuO, and Ag were synthesized and characterized by spectroscopy and microscopy, presenting average sizes < 25 nm and spherical shapes. ZnO-NPs did not present antifungal activity at the assayed conditions, while the minimum fungicidal concentrations (MFCs) were 1000 and 10 µg mL−1 for CuO-NPs and Ag-NPs, respectively. The NPs’ antimicrobial action included conidial membrane permeability and strong intracellular disorganization. Moreover, the Ag-NPs reduced green mold incidence on inoculated lemons when applied to the fruit. Taken together, Ag-NPs were effective in inhibiting both fungicide-sensitive and -resistant isolates of the main lemon postharvest pathogens, suggesting their potential use as an alternative approach.
Fil: Baigorria, Carina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Debes, Mario Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Ledesma, Ana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Alastuey, Patricio. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; Argentina
Fil: Tirado, Monica Cecilia. Universidad Nacional de Tucumán. Instituto de Física del Noroeste Argentino. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Física del Noroeste Argentino; Argentina
Fil: Volentini, Sabrina Inès. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Postharvest fungal diseases are the main cause of economic losses in lemon production. The continued use of synthetic fungicides to control the diseases favors the emergence of resistant strains, which encourages the search for alternatives. The aim of this study was to assess the efficacy of metallic nanoparticles (NPs) as antifungal agents against local isolates of Penicillium digitatum and Penicillium italicum, each of them in a fungicide-sensitive and -resistant version, and a Geotrichum citri-aurantii isolate. NPs of ZnO, CuO, and Ag were synthesized and characterized by spectroscopy and microscopy, presenting average sizes < 25 nm and spherical shapes. ZnO-NPs did not present antifungal activity at the assayed conditions, while the minimum fungicidal concentrations (MFCs) were 1000 and 10 µg mL−1 for CuO-NPs and Ag-NPs, respectively. The NPs’ antimicrobial action included conidial membrane permeability and strong intracellular disorganization. Moreover, the Ag-NPs reduced green mold incidence on inoculated lemons when applied to the fruit. Taken together, Ag-NPs were effective in inhibiting both fungicide-sensitive and -resistant isolates of the main lemon postharvest pathogens, suggesting their potential use as an alternative approach.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256462
Baigorria, Carina Giselle; Cerioni, Luciana; Debes, Mario Alberto; Ledesma, Ana Estela; Alastuey, Patricio; et al.; Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases; MDPI; Journal of Fungi; 10; 11; 11-2024; 1-15
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256462
identifier_str_mv Baigorria, Carina Giselle; Cerioni, Luciana; Debes, Mario Alberto; Ledesma, Ana Estela; Alastuey, Patricio; et al.; Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases; MDPI; Journal of Fungi; 10; 11; 11-2024; 1-15
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/10/11/782
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof10110782
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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