Phosphorous pentoxide-free bioactive glass exhibits dose-dependent angiogenic and osteogenic capacities which are retained in glass polymeric composite scaffolds

• Autores: Font Tellado, Sonia; Delgado, José Angel; Poh, Su Ping Patrina; Zhang, Wen; García Vallés, Maite; Martínez, Salvador; Gorustovich Alonso, Alejandro Adrian; Morejón, Lizette; van Griensven, Martijn; Balmayor, Elizabeth Rosado
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: BIOACTIVE GLASS; ANGIOGENESIS; OSTEOGENESIS; GLASS POLYMERIC COMPOSITE SCAFFOLDS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Bioactive glasses (BGs) are attractive materials for bone tissue engineering because of their bioactivity and osteoinductivity. In this study, we report the synthesis of a novel phosphorous pentoxide-free, silicate-based bioactive glass (52S-BG) composed of 52.1% SiO2, 23.2% Na2O and 22.6% CaO (wt%). The glass was thoroughly characterized. The biocompatibility and osteogenic properties of 52S-BG particles were analyzed in vitro with human adipose-derived mesenchymal stem cells (AdMSCs) and human osteoblasts. 52S-BG particles were biocompatible and induced mineralized matrix deposition and the expression of osteogenic markers (RunX2, alkaline phosphatase, osteocalcin, osteopontin, collagen I) and the angiogenic marker vascular endothelial growth factor (VEGF). Angiogenic properties were additionally confirmed in a zebrafish embryo model. 52S-BG was added to poly-ϵ-caprolactone (PCL) to obtain a composite with 10 wt% glass content. Composite PCL/52S-BG scaffolds were fabricated by additive manufacturing and displayed high porosity (76%) and pore interconnectivity. The incorporation of 52S-BG particles increased the Young's modulus of PCL scaffolds from 180 to 230 MPa. AdMSC seeding efficiency and proliferation were higher in PCL/52S-BG compared to PCL scaffolds, indicating improved biocompatibility. Finally, 52S-BG incorporation improved the scaffolds' osteogenic and angiogenic properties by increasing mineral deposition and inducing relevant gene expression and VEGF protein secretion. Overall, 52S-BG particles and PCL/52S-BG composites may be attractive for diverse bone engineering applications requiring concomitant angiogenic properties. This journal is
Fil: Font Tellado, Sonia. Universitat Technical Zu Munich; Alemania
Fil: Delgado, José Angel. Universitat Internacional de Catalunya; España. University of Havana; Cuba
Fil: Poh, Su Ping Patrina. Universität zu Berlin; Alemania. Universitat Technical Zu Munich; Alemania
Fil: Zhang, Wen. Universitat Technical Zu Munich; Alemania. Ethris GmbH; Alemania
Fil: García Vallés, Maite. Universidad de Barcelona; España
Fil: Martínez, Salvador. Universidad de La Habana; Cuba. Universidad de Barcelona; España
Fil: Gorustovich Alonso, Alejandro Adrian. Universidad Católica de Salta. Facultad de Ingeniería e Informática; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina
Fil: Morejón, Lizette. Universidad de La Habana; Cuba
Fil: van Griensven, Martijn. Universitat Technical Zu Munich; Alemania. Universiteit Maastricht. Faculty of Health Medicine and Life Sciences. Institute for Technology Inspired Regenerative Medicine; Países Bajos
Fil: Balmayor, Elizabeth Rosado. Universitat Technical Zu Munich; Alemania. Universiteit Maastricht. Faculty of Health Medicine and Life Sciences. Institute for Technology Inspired Regenerative Medicine; Países Bajos