Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia

Autores
Haro Durand, Luis Alberto; Baldi, Alberto; Fanovich, Maria Alejandra; Boccaccini, Aldo Roberto; Gorustovich Alonso, Alejandro Adrian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
One of the main complications of diabetes mellitus (DM) is the failure in the process of tissue repair due to cellular dysfunction as a result of hyperglycemia. Under hyperglycemic conditions, the main cells involved in restoring the functionality and integrity of tissues are unable to migrate, proliferate, and secrete growth factors and components of the extracellular matrix, all of which is favored by altered mechanisms of glycation and oxidation. This alteration invariably contributes to failures in repair processes. Thus, it is of biomedical interest to study different therapeutic strategies to optimize the repair and/or regeneration of tissues under hyperglycemic conditions. These strategies may include the use of different biomaterials in the form of three-dimensional porous matrices, known as scaffolds. The ideal design of these scaffolds should provide temporary biocompatible mechanical support and positively modulate the cellular response. Since it has been established that boron (B) plays a role in angiogenesis and tissue repair, it can be expected that the controlled and localized release of B ions from the bioactive glass-ceramic scaffolds could represent a promising alternative therapy in regenerative medicine of vascularized tissues in patients with DM. The aim of this work was to study the in vitro cellular response of ionic dissolution products from bioactive scaffolds manufactured from the controlled crystallization of a 45S5 glass (% w/w composition: 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5) added with 2% of B2O3 (45S5.2B), in primary cultures of fibroblasts (HDF) and endothelial cells (HUVECs) grown in hyperglycemic conditions (30 mM D-glucose). The results demonstrated, for the first time, that the ionic dissolution products released from the 45S5.2B bioactive glass-ceramic scaffolds positively modulate the in vitro cellular response of fibroblasts and endothelial cells grown under hyperglycemic conditions. This was corroborated by an increased proliferative and migratory response, greater ability to form tubules in vitro and an increase in the secretion of growth factors. These findings may be relevant in vascularized tissue engineering since scaffolds obtained from the 45S5.2B bioactive glass could act as inorganic agents that positively modulate the cellular response and favor processes of tissue repair and/or regeneration in patients with DM.
Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; Argentina
Fil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo Roberto. Universidad Católica de Salta; Argentina
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; Argentina
XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de Biología
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biociencias
Materia
BIOACTIVE GLASS-CERAMIC
SCAFFOLDS
BORON
HYPERGLYCEMIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161077
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/161077
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemiaHaro Durand, Luis AlbertoBaldi, AlbertoFanovich, Maria AlejandraBoccaccini, Aldo RobertoGorustovich Alonso, Alejandro AdrianBIOACTIVE GLASS-CERAMICSCAFFOLDSBORONHYPERGLYCEMIAhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3One of the main complications of diabetes mellitus (DM) is the failure in the process of tissue repair due to cellular dysfunction as a result of hyperglycemia. Under hyperglycemic conditions, the main cells involved in restoring the functionality and integrity of tissues are unable to migrate, proliferate, and secrete growth factors and components of the extracellular matrix, all of which is favored by altered mechanisms of glycation and oxidation. This alteration invariably contributes to failures in repair processes. Thus, it is of biomedical interest to study different therapeutic strategies to optimize the repair and/or regeneration of tissues under hyperglycemic conditions. These strategies may include the use of different biomaterials in the form of three-dimensional porous matrices, known as scaffolds. The ideal design of these scaffolds should provide temporary biocompatible mechanical support and positively modulate the cellular response. Since it has been established that boron (B) plays a role in angiogenesis and tissue repair, it can be expected that the controlled and localized release of B ions from the bioactive glass-ceramic scaffolds could represent a promising alternative therapy in regenerative medicine of vascularized tissues in patients with DM. The aim of this work was to study the in vitro cellular response of ionic dissolution products from bioactive scaffolds manufactured from the controlled crystallization of a 45S5 glass (% w/w composition: 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5) added with 2% of B2O3 (45S5.2B), in primary cultures of fibroblasts (HDF) and endothelial cells (HUVECs) grown in hyperglycemic conditions (30 mM D-glucose). The results demonstrated, for the first time, that the ionic dissolution products released from the 45S5.2B bioactive glass-ceramic scaffolds positively modulate the in vitro cellular response of fibroblasts and endothelial cells grown under hyperglycemic conditions. This was corroborated by an increased proliferative and migratory response, greater ability to form tubules in vitro and an increase in the secretion of growth factors. These findings may be relevant in vascularized tissue engineering since scaffolds obtained from the 45S5.2B bioactive glass could act as inorganic agents that positively modulate the cellular response and favor processes of tissue repair and/or regeneration in patients with DM.Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; ArgentinaFil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo Roberto. Universidad Católica de Salta; ArgentinaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; ArgentinaXX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de BiologíaBuenos AiresArgentinaSociedad Argentina de BiologíaSociedad Uruguaya de BiocienciasTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161077Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia; XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de Biología; Buenos Aires; Argentina; 2018; 44-450327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.3/33866Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:56Zoai:ri.conicet.gov.ar:11336/161077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:56.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
title Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
spellingShingle Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
Haro Durand, Luis Alberto
BIOACTIVE GLASS-CERAMIC
SCAFFOLDS
BORON
HYPERGLYCEMIA
title_short Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
title_full Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
title_fullStr Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
title_full_unstemmed Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
title_sort Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia
dc.creator.none.fl_str_mv Haro Durand, Luis Alberto
Baldi, Alberto
Fanovich, Maria Alejandra
Boccaccini, Aldo Roberto
Gorustovich Alonso, Alejandro Adrian
author Haro Durand, Luis Alberto
author_facet Haro Durand, Luis Alberto
Baldi, Alberto
Fanovich, Maria Alejandra
Boccaccini, Aldo Roberto
Gorustovich Alonso, Alejandro Adrian
author_role author
author2 Baldi, Alberto
Fanovich, Maria Alejandra
Boccaccini, Aldo Roberto
Gorustovich Alonso, Alejandro Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOACTIVE GLASS-CERAMIC
SCAFFOLDS
BORON
HYPERGLYCEMIA
topic BIOACTIVE GLASS-CERAMIC
SCAFFOLDS
BORON
HYPERGLYCEMIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv One of the main complications of diabetes mellitus (DM) is the failure in the process of tissue repair due to cellular dysfunction as a result of hyperglycemia. Under hyperglycemic conditions, the main cells involved in restoring the functionality and integrity of tissues are unable to migrate, proliferate, and secrete growth factors and components of the extracellular matrix, all of which is favored by altered mechanisms of glycation and oxidation. This alteration invariably contributes to failures in repair processes. Thus, it is of biomedical interest to study different therapeutic strategies to optimize the repair and/or regeneration of tissues under hyperglycemic conditions. These strategies may include the use of different biomaterials in the form of three-dimensional porous matrices, known as scaffolds. The ideal design of these scaffolds should provide temporary biocompatible mechanical support and positively modulate the cellular response. Since it has been established that boron (B) plays a role in angiogenesis and tissue repair, it can be expected that the controlled and localized release of B ions from the bioactive glass-ceramic scaffolds could represent a promising alternative therapy in regenerative medicine of vascularized tissues in patients with DM. The aim of this work was to study the in vitro cellular response of ionic dissolution products from bioactive scaffolds manufactured from the controlled crystallization of a 45S5 glass (% w/w composition: 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5) added with 2% of B2O3 (45S5.2B), in primary cultures of fibroblasts (HDF) and endothelial cells (HUVECs) grown in hyperglycemic conditions (30 mM D-glucose). The results demonstrated, for the first time, that the ionic dissolution products released from the 45S5.2B bioactive glass-ceramic scaffolds positively modulate the in vitro cellular response of fibroblasts and endothelial cells grown under hyperglycemic conditions. This was corroborated by an increased proliferative and migratory response, greater ability to form tubules in vitro and an increase in the secretion of growth factors. These findings may be relevant in vascularized tissue engineering since scaffolds obtained from the 45S5.2B bioactive glass could act as inorganic agents that positively modulate the cellular response and favor processes of tissue repair and/or regeneration in patients with DM.
Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; Argentina
Fil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo Roberto. Universidad Católica de Salta; Argentina
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Catolica de Salta. Facultad de Ingeniería E Informatica. Instituto D/estudios Interdiciplinarios En Ingeniería; Argentina
XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de Biología
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biociencias
description One of the main complications of diabetes mellitus (DM) is the failure in the process of tissue repair due to cellular dysfunction as a result of hyperglycemia. Under hyperglycemic conditions, the main cells involved in restoring the functionality and integrity of tissues are unable to migrate, proliferate, and secrete growth factors and components of the extracellular matrix, all of which is favored by altered mechanisms of glycation and oxidation. This alteration invariably contributes to failures in repair processes. Thus, it is of biomedical interest to study different therapeutic strategies to optimize the repair and/or regeneration of tissues under hyperglycemic conditions. These strategies may include the use of different biomaterials in the form of three-dimensional porous matrices, known as scaffolds. The ideal design of these scaffolds should provide temporary biocompatible mechanical support and positively modulate the cellular response. Since it has been established that boron (B) plays a role in angiogenesis and tissue repair, it can be expected that the controlled and localized release of B ions from the bioactive glass-ceramic scaffolds could represent a promising alternative therapy in regenerative medicine of vascularized tissues in patients with DM. The aim of this work was to study the in vitro cellular response of ionic dissolution products from bioactive scaffolds manufactured from the controlled crystallization of a 45S5 glass (% w/w composition: 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5) added with 2% of B2O3 (45S5.2B), in primary cultures of fibroblasts (HDF) and endothelial cells (HUVECs) grown in hyperglycemic conditions (30 mM D-glucose). The results demonstrated, for the first time, that the ionic dissolution products released from the 45S5.2B bioactive glass-ceramic scaffolds positively modulate the in vitro cellular response of fibroblasts and endothelial cells grown under hyperglycemic conditions. This was corroborated by an increased proliferative and migratory response, greater ability to form tubules in vitro and an increase in the secretion of growth factors. These findings may be relevant in vascularized tissue engineering since scaffolds obtained from the 45S5.2B bioactive glass could act as inorganic agents that positively modulate the cellular response and favor processes of tissue repair and/or regeneration in patients with DM.
publishDate 2019
dc.date.none.fl_str_mv 2019
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info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161077
Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia; XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de Biología; Buenos Aires; Argentina; 2018; 44-45
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161077
identifier_str_mv Ionic dissolution products from bioactive glass-ceramic scaffolds 45S5.2B positively modulate the in vitro cell response under conditions of hyperglycemia; XX Jornadas Anuales de la Sociedad Argentina de Biología y XVII Jornadas de la Sociedad Uruguaya de Biociencias; Segundas Jornadas Rioplatenses de Biología; Buenos Aires; Argentina; 2018; 44-45
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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publisher.none.fl_str_mv Tech Science Press
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