Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering

Autores
Gorustovich Alonso, Alejandro Adrian; Vargas, Gabriela Elizabet; Bretcanu, O.; Vera Mesones, Rosa; Porto Lopez, Jose Manuel; Boccaccini, Aldo Roberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to investigate a novel ex ovo bioassay for the first time using the chick embryo chorioallantoic membrane (CAM) for testing tissue engineering bioceramic scaffolds. Bioglass based scaffolds with porosity in the range of 90–95% were fabricated using the foam replica technique and sintering at 1100°C for 1 h. Scaffolds (5 × 5 × 2 mm3) were placed on the CAM at 10 days of total incubation. The embryos were killed 5 days after implantation. The scaffolds and CAM were explanted, fixed in formalin solution and processed for embedding in methyl methacrylate. Histological analysis using ground sections showed that the scaffolds were surrounded by CAM. There was no occurrence of macrophages or related inflammatory cells. The results described in this paper indicate that the developed bioassay is an appropriate approach as an alternative to conventional animal models to evaluate the biocompatibility of scaffold biomaterials for tissue engineering and regenerative medicine.
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Vargas, Gabriela Elizabet. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bretcanu, O.. Imperial College London; Reino Unido
Fil: Vera Mesones, Rosa. Universidad Católica de Salta; Argentina
Fil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo Roberto. Imperial College London; Reino Unido
Materia
SCAFFOLDS
BIOACTIVE GLASS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243395
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineeringGorustovich Alonso, Alejandro AdrianVargas, Gabriela ElizabetBretcanu, O.Vera Mesones, RosaPorto Lopez, Jose ManuelBoccaccini, Aldo RobertoSCAFFOLDSBIOACTIVE GLASShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The aim of the present study was to investigate a novel ex ovo bioassay for the first time using the chick embryo chorioallantoic membrane (CAM) for testing tissue engineering bioceramic scaffolds. Bioglass based scaffolds with porosity in the range of 90–95% were fabricated using the foam replica technique and sintering at 1100°C for 1 h. Scaffolds (5 × 5 × 2 mm3) were placed on the CAM at 10 days of total incubation. The embryos were killed 5 days after implantation. The scaffolds and CAM were explanted, fixed in formalin solution and processed for embedding in methyl methacrylate. Histological analysis using ground sections showed that the scaffolds were surrounded by CAM. There was no occurrence of macrophages or related inflammatory cells. The results described in this paper indicate that the developed bioassay is an appropriate approach as an alternative to conventional animal models to evaluate the biocompatibility of scaffold biomaterials for tissue engineering and regenerative medicine.Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; ArgentinaFil: Vargas, Gabriela Elizabet. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bretcanu, O.. Imperial College London; Reino UnidoFil: Vera Mesones, Rosa. Universidad Católica de Salta; ArgentinaFil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo Roberto. Imperial College London; Reino UnidoManey Publishing2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243395Gorustovich Alonso, Alejandro Adrian; Vargas, Gabriela Elizabet; Bretcanu, O.; Vera Mesones, Rosa; Porto Lopez, Jose Manuel; et al.; Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering; Maney Publishing; Advances In Applied Ceramics; 107; 5; 7-2013; 274-2761743-6753CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/174367508X306541info:eu-repo/semantics/altIdentifier/doi/10.1179/174367508X306541info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:08Zoai:ri.conicet.gov.ar:11336/243395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:08.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
title Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
spellingShingle Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
Gorustovich Alonso, Alejandro Adrian
SCAFFOLDS
BIOACTIVE GLASS
title_short Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
title_full Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
title_fullStr Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
title_full_unstemmed Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
title_sort Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering
dc.creator.none.fl_str_mv Gorustovich Alonso, Alejandro Adrian
Vargas, Gabriela Elizabet
Bretcanu, O.
Vera Mesones, Rosa
Porto Lopez, Jose Manuel
Boccaccini, Aldo Roberto
author Gorustovich Alonso, Alejandro Adrian
author_facet Gorustovich Alonso, Alejandro Adrian
Vargas, Gabriela Elizabet
Bretcanu, O.
Vera Mesones, Rosa
Porto Lopez, Jose Manuel
Boccaccini, Aldo Roberto
author_role author
author2 Vargas, Gabriela Elizabet
Bretcanu, O.
Vera Mesones, Rosa
Porto Lopez, Jose Manuel
Boccaccini, Aldo Roberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SCAFFOLDS
BIOACTIVE GLASS
topic SCAFFOLDS
BIOACTIVE GLASS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present study was to investigate a novel ex ovo bioassay for the first time using the chick embryo chorioallantoic membrane (CAM) for testing tissue engineering bioceramic scaffolds. Bioglass based scaffolds with porosity in the range of 90–95% were fabricated using the foam replica technique and sintering at 1100°C for 1 h. Scaffolds (5 × 5 × 2 mm3) were placed on the CAM at 10 days of total incubation. The embryos were killed 5 days after implantation. The scaffolds and CAM were explanted, fixed in formalin solution and processed for embedding in methyl methacrylate. Histological analysis using ground sections showed that the scaffolds were surrounded by CAM. There was no occurrence of macrophages or related inflammatory cells. The results described in this paper indicate that the developed bioassay is an appropriate approach as an alternative to conventional animal models to evaluate the biocompatibility of scaffold biomaterials for tissue engineering and regenerative medicine.
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Vargas, Gabriela Elizabet. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bretcanu, O.. Imperial College London; Reino Unido
Fil: Vera Mesones, Rosa. Universidad Católica de Salta; Argentina
Fil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo Roberto. Imperial College London; Reino Unido
description The aim of the present study was to investigate a novel ex ovo bioassay for the first time using the chick embryo chorioallantoic membrane (CAM) for testing tissue engineering bioceramic scaffolds. Bioglass based scaffolds with porosity in the range of 90–95% were fabricated using the foam replica technique and sintering at 1100°C for 1 h. Scaffolds (5 × 5 × 2 mm3) were placed on the CAM at 10 days of total incubation. The embryos were killed 5 days after implantation. The scaffolds and CAM were explanted, fixed in formalin solution and processed for embedding in methyl methacrylate. Histological analysis using ground sections showed that the scaffolds were surrounded by CAM. There was no occurrence of macrophages or related inflammatory cells. The results described in this paper indicate that the developed bioassay is an appropriate approach as an alternative to conventional animal models to evaluate the biocompatibility of scaffold biomaterials for tissue engineering and regenerative medicine.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243395
Gorustovich Alonso, Alejandro Adrian; Vargas, Gabriela Elizabet; Bretcanu, O.; Vera Mesones, Rosa; Porto Lopez, Jose Manuel; et al.; Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering; Maney Publishing; Advances In Applied Ceramics; 107; 5; 7-2013; 274-276
1743-6753
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243395
identifier_str_mv Gorustovich Alonso, Alejandro Adrian; Vargas, Gabriela Elizabet; Bretcanu, O.; Vera Mesones, Rosa; Porto Lopez, Jose Manuel; et al.; Novel bioassay to evaluate biocompatibility of bioactive glass scaffolds for tissue engineering; Maney Publishing; Advances In Applied Ceramics; 107; 5; 7-2013; 274-276
1743-6753
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/174367508X306541
info:eu-repo/semantics/altIdentifier/doi/10.1179/174367508X306541
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Maney Publishing
publisher.none.fl_str_mv Maney Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613523229900800
score 13.070432

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