Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling

Autores
Sampson, James F.; Suryawanshi, Amol; Chen, Wei Sheng; Rabinovich, Gabriel Adrián; Panjwani, Noorjahan
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases.
Fil: Sampson, James F.. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados Unidos
Fil: Suryawanshi, Amol. Tufts University School of Medicine; Estados Unidos
Fil: Chen, Wei Sheng. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados Unidos
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Panjwani, Noorjahan. Tufts University School of Medicine; Estados Unidos
Materia
Galectin 8
T Cells
Tolerance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22979
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signalingSampson, James F.Suryawanshi, AmolChen, Wei ShengRabinovich, Gabriel AdriánPanjwani, NoorjahanGalectin 8T CellsTolerancehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases.Fil: Sampson, James F.. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados UnidosFil: Suryawanshi, Amol. Tufts University School of Medicine; Estados UnidosFil: Chen, Wei Sheng. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados UnidosFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Panjwani, Noorjahan. Tufts University School of Medicine; Estados UnidosAustralian Society for Immunology2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22979Sampson, James F.; Suryawanshi, Amol; Chen, Wei Sheng; Rabinovich, Gabriel Adrián; Panjwani, Noorjahan; Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling; Australian Society for Immunology; Immunology and Cell Biology; 94; 2; 2-2016; 213-2190818-96411440-1711CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/icb/journal/v94/n2/full/icb201572a.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/icb.2015.72info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747822/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:16Zoai:ri.conicet.gov.ar:11336/22979instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:16.364CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
title Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
spellingShingle Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
Sampson, James F.
Galectin 8
T Cells
Tolerance
title_short Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
title_full Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
title_fullStr Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
title_full_unstemmed Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
title_sort Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
dc.creator.none.fl_str_mv Sampson, James F.
Suryawanshi, Amol
Chen, Wei Sheng
Rabinovich, Gabriel Adrián
Panjwani, Noorjahan
author Sampson, James F.
author_facet Sampson, James F.
Suryawanshi, Amol
Chen, Wei Sheng
Rabinovich, Gabriel Adrián
Panjwani, Noorjahan
author_role author
author2 Suryawanshi, Amol
Chen, Wei Sheng
Rabinovich, Gabriel Adrián
Panjwani, Noorjahan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Galectin 8
T Cells
Tolerance
topic Galectin 8
T Cells
Tolerance
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases.
Fil: Sampson, James F.. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados Unidos
Fil: Suryawanshi, Amol. Tufts University School of Medicine; Estados Unidos
Fil: Chen, Wei Sheng. Tufts University. Sackler School of Graduate Biomedical Sciences; Estados Unidos
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Panjwani, Noorjahan. Tufts University School of Medicine; Estados Unidos
description Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22979
Sampson, James F.; Suryawanshi, Amol; Chen, Wei Sheng; Rabinovich, Gabriel Adrián; Panjwani, Noorjahan; Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling; Australian Society for Immunology; Immunology and Cell Biology; 94; 2; 2-2016; 213-219
0818-9641
1440-1711
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22979
identifier_str_mv Sampson, James F.; Suryawanshi, Amol; Chen, Wei Sheng; Rabinovich, Gabriel Adrián; Panjwani, Noorjahan; Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling; Australian Society for Immunology; Immunology and Cell Biology; 94; 2; 2-2016; 213-219
0818-9641
1440-1711
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/icb/journal/v94/n2/full/icb201572a.html
info:eu-repo/semantics/altIdentifier/doi/10.1038/icb.2015.72
info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747822/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Australian Society for Immunology
publisher.none.fl_str_mv Australian Society for Immunology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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