Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery

Autores
Clemente, Tiago; Vieira, Narcisio J.; Cerliani, Juan Pablo; Adrain, Colin; Luthi, Alexander; Dominguez, Mariana; Yon, Monica; Barrence, Fernanda C.; Riul, Thalita B.; Cummings, Richard D.; Zorn, Thelma; Amigorena, Sebastian; Dias Baruffi, Marcelo; Rodriguez, Mauricio M.; Seamus, J. Martin; Rabinovich, Gabriel Adrián; Amarante Mendez, Gustavo P.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Secretory granules released by cytotoxic T lymphocytes (CTLs) are powerful weapons against intracellular microbes and tumor cells. Despite significant progress, there is still limited information on the molecular mechanisms implicated in target-driven degranulation, effector cell survival and composition and structure of the lytic granules. Here, using a proteomic approach we identified a panel of putative cytotoxic granule proteins, including some already known granule constituents and novel proteins that contribute to regulate the CTL lytic machinery. Particularly, we identified galectin-1 (Gal1), an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. Mechanistic studies revealed the ability of Gal1 to control the non-secretory lytic pathway by influencing Fas?Fas ligand interactions. This study offers new insights on the composition of the cytotoxic granule machinery, highlighting the dynamic cross talk between secretory and non-secretory pathways in controlling CTL lytic function.
Fil: Clemente, Tiago. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
Fil: Vieira, Narcisio J.. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
Fil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Adrain, Colin. Instituto Gulbenkian de Ciência; Portugal
Fil: Luthi, Alexander. Trinity College; Irlanda
Fil: Dominguez, Mariana. Universidade de Sao Paulo; Brasil
Fil: Yon, Monica. Universidade de Sao Paulo; Brasil
Fil: Barrence, Fernanda C.. Universidade de Sao Paulo; Brasil
Fil: Riul, Thalita B.. Universidade de Sao Paulo; Brasil
Fil: Cummings, Richard D.. Harvard Medical School; Estados Unidos
Fil: Zorn, Thelma. Universidade de Sao Paulo; Brasil
Fil: Amigorena, Sebastian. Inserm; Francia
Fil: Dias Baruffi, Marcelo. Universidade de Sao Paulo; Brasil
Fil: Rodriguez, Mauricio M.. Universidade de Sao Paulo; Brasil
Fil: Seamus, J. Martin. Trinity College; Irlanda
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Amarante Mendez, Gustavo P.. Trinity College; Irlanda. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
Materia
GALECTIN-1
T CELLS
GLYCANS
PROTEOMIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52134
Acceder
id CONICETDig_d1af963cd78a0c5f738e4bf336c75d45
oai_identifier_str oai:ri.conicet.gov.ar:11336/52134
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machineryClemente, TiagoVieira, Narcisio J.Cerliani, Juan PabloAdrain, ColinLuthi, AlexanderDominguez, MarianaYon, MonicaBarrence, Fernanda C.Riul, Thalita B.Cummings, Richard D.Zorn, ThelmaAmigorena, SebastianDias Baruffi, MarceloRodriguez, Mauricio M.Seamus, J. MartinRabinovich, Gabriel AdriánAmarante Mendez, Gustavo P.GALECTIN-1T CELLSGLYCANSPROTEOMIChttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Secretory granules released by cytotoxic T lymphocytes (CTLs) are powerful weapons against intracellular microbes and tumor cells. Despite significant progress, there is still limited information on the molecular mechanisms implicated in target-driven degranulation, effector cell survival and composition and structure of the lytic granules. Here, using a proteomic approach we identified a panel of putative cytotoxic granule proteins, including some already known granule constituents and novel proteins that contribute to regulate the CTL lytic machinery. Particularly, we identified galectin-1 (Gal1), an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. Mechanistic studies revealed the ability of Gal1 to control the non-secretory lytic pathway by influencing Fas?Fas ligand interactions. This study offers new insights on the composition of the cytotoxic granule machinery, highlighting the dynamic cross talk between secretory and non-secretory pathways in controlling CTL lytic function.Fil: Clemente, Tiago. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; BrasilFil: Vieira, Narcisio J.. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; BrasilFil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Adrain, Colin. Instituto Gulbenkian de Ciência; PortugalFil: Luthi, Alexander. Trinity College; IrlandaFil: Dominguez, Mariana. Universidade de Sao Paulo; BrasilFil: Yon, Monica. Universidade de Sao Paulo; BrasilFil: Barrence, Fernanda C.. Universidade de Sao Paulo; BrasilFil: Riul, Thalita B.. Universidade de Sao Paulo; BrasilFil: Cummings, Richard D.. Harvard Medical School; Estados UnidosFil: Zorn, Thelma. Universidade de Sao Paulo; BrasilFil: Amigorena, Sebastian. Inserm; FranciaFil: Dias Baruffi, Marcelo. Universidade de Sao Paulo; BrasilFil: Rodriguez, Mauricio M.. Universidade de Sao Paulo; BrasilFil: Seamus, J. Martin. Trinity College; IrlandaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Amarante Mendez, Gustavo P.. Trinity College; Irlanda. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; BrasilNature Publishing Group2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52134Clemente, Tiago; Vieira, Narcisio J.; Cerliani, Juan Pablo; Adrain, Colin; Luthi, Alexander; et al.; Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery; Nature Publishing Group; Cell Death and Disease; 8; 12; 12-2017; 1-102041-48892041-4889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/doifinder/10.1038/cddis.2017.506info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2017.506info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827204/info:eu-repo/semantics/altIdentifier/pmid/29215607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:30Zoai:ri.conicet.gov.ar:11336/52134instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:30.653CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
title Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
spellingShingle Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
Clemente, Tiago
GALECTIN-1
T CELLS
GLYCANS
PROTEOMIC
title_short Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
title_full Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
title_fullStr Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
title_full_unstemmed Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
title_sort Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery
dc.creator.none.fl_str_mv Clemente, Tiago
Vieira, Narcisio J.
Cerliani, Juan Pablo
Adrain, Colin
Luthi, Alexander
Dominguez, Mariana
Yon, Monica
Barrence, Fernanda C.
Riul, Thalita B.
Cummings, Richard D.
Zorn, Thelma
Amigorena, Sebastian
Dias Baruffi, Marcelo
Rodriguez, Mauricio M.
Seamus, J. Martin
Rabinovich, Gabriel Adrián
Amarante Mendez, Gustavo P.
author Clemente, Tiago
author_facet Clemente, Tiago
Vieira, Narcisio J.
Cerliani, Juan Pablo
Adrain, Colin
Luthi, Alexander
Dominguez, Mariana
Yon, Monica
Barrence, Fernanda C.
Riul, Thalita B.
Cummings, Richard D.
Zorn, Thelma
Amigorena, Sebastian
Dias Baruffi, Marcelo
Rodriguez, Mauricio M.
Seamus, J. Martin
Rabinovich, Gabriel Adrián
Amarante Mendez, Gustavo P.
author_role author
author2 Vieira, Narcisio J.
Cerliani, Juan Pablo
Adrain, Colin
Luthi, Alexander
Dominguez, Mariana
Yon, Monica
Barrence, Fernanda C.
Riul, Thalita B.
Cummings, Richard D.
Zorn, Thelma
Amigorena, Sebastian
Dias Baruffi, Marcelo
Rodriguez, Mauricio M.
Seamus, J. Martin
Rabinovich, Gabriel Adrián
Amarante Mendez, Gustavo P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GALECTIN-1
T CELLS
GLYCANS
PROTEOMIC
topic GALECTIN-1
T CELLS
GLYCANS
PROTEOMIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Secretory granules released by cytotoxic T lymphocytes (CTLs) are powerful weapons against intracellular microbes and tumor cells. Despite significant progress, there is still limited information on the molecular mechanisms implicated in target-driven degranulation, effector cell survival and composition and structure of the lytic granules. Here, using a proteomic approach we identified a panel of putative cytotoxic granule proteins, including some already known granule constituents and novel proteins that contribute to regulate the CTL lytic machinery. Particularly, we identified galectin-1 (Gal1), an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. Mechanistic studies revealed the ability of Gal1 to control the non-secretory lytic pathway by influencing Fas?Fas ligand interactions. This study offers new insights on the composition of the cytotoxic granule machinery, highlighting the dynamic cross talk between secretory and non-secretory pathways in controlling CTL lytic function.
Fil: Clemente, Tiago. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
Fil: Vieira, Narcisio J.. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
Fil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Adrain, Colin. Instituto Gulbenkian de Ciência; Portugal
Fil: Luthi, Alexander. Trinity College; Irlanda
Fil: Dominguez, Mariana. Universidade de Sao Paulo; Brasil
Fil: Yon, Monica. Universidade de Sao Paulo; Brasil
Fil: Barrence, Fernanda C.. Universidade de Sao Paulo; Brasil
Fil: Riul, Thalita B.. Universidade de Sao Paulo; Brasil
Fil: Cummings, Richard D.. Harvard Medical School; Estados Unidos
Fil: Zorn, Thelma. Universidade de Sao Paulo; Brasil
Fil: Amigorena, Sebastian. Inserm; Francia
Fil: Dias Baruffi, Marcelo. Universidade de Sao Paulo; Brasil
Fil: Rodriguez, Mauricio M.. Universidade de Sao Paulo; Brasil
Fil: Seamus, J. Martin. Trinity College; Irlanda
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Amarante Mendez, Gustavo P.. Trinity College; Irlanda. Universidade de Sao Paulo; Brasil. Instituto Nacional de Ciência e Tecnologia ; Brasil
description Secretory granules released by cytotoxic T lymphocytes (CTLs) are powerful weapons against intracellular microbes and tumor cells. Despite significant progress, there is still limited information on the molecular mechanisms implicated in target-driven degranulation, effector cell survival and composition and structure of the lytic granules. Here, using a proteomic approach we identified a panel of putative cytotoxic granule proteins, including some already known granule constituents and novel proteins that contribute to regulate the CTL lytic machinery. Particularly, we identified galectin-1 (Gal1), an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. Mechanistic studies revealed the ability of Gal1 to control the non-secretory lytic pathway by influencing Fas?Fas ligand interactions. This study offers new insights on the composition of the cytotoxic granule machinery, highlighting the dynamic cross talk between secretory and non-secretory pathways in controlling CTL lytic function.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52134
Clemente, Tiago; Vieira, Narcisio J.; Cerliani, Juan Pablo; Adrain, Colin; Luthi, Alexander; et al.; Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery; Nature Publishing Group; Cell Death and Disease; 8; 12; 12-2017; 1-10
2041-4889
2041-4889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52134
identifier_str_mv Clemente, Tiago; Vieira, Narcisio J.; Cerliani, Juan Pablo; Adrain, Colin; Luthi, Alexander; et al.; Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery; Nature Publishing Group; Cell Death and Disease; 8; 12; 12-2017; 1-10
2041-4889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/doifinder/10.1038/cddis.2017.506
info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2017.506
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827204/
info:eu-repo/semantics/altIdentifier/pmid/29215607
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269289298526208
score 13.13397

Publicaciones similares