Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA

Autores
de Freitas, Milena V.; Ariede, Raquel B.; Hata, Milene E.; Mastrochirico Filho, Vito A.; del Pazo, Felipe; Villanova, Gabriela Vanina; Mendonça, Fernando F.; Foresti, Fábio Porto; Hashimoto, Diogo T.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.
Fil: de Freitas, Milena V.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Ariede, Raquel B.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Hata, Milene E.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Mastrochirico Filho, Vito A.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: del Pazo, Felipe. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Villanova, Gabriela Vanina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mendonça, Fernando F.. Universidade Federal de Sao Paulo; Brasil
Fil: Foresti, Fábio Porto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Hashimoto, Diogo T.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
AQUACULTURE
CONTROL-REGION
GENETIC BREEDING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157146
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNAde Freitas, Milena V.Ariede, Raquel B.Hata, Milene E.Mastrochirico Filho, Vito A.del Pazo, FelipeVillanova, Gabriela VaninaMendonça, Fernando F.Foresti, Fábio PortoHashimoto, Diogo T.AQUACULTURECONTROL-REGIONGENETIC BREEDINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.Fil: de Freitas, Milena V.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; BrasilFil: Ariede, Raquel B.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; BrasilFil: Hata, Milene E.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; BrasilFil: Mastrochirico Filho, Vito A.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; BrasilFil: del Pazo, Felipe. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Villanova, Gabriela Vanina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Mendonça, Fernando F.. Universidade Federal de Sao Paulo; BrasilFil: Foresti, Fábio Porto. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Hashimoto, Diogo T.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilSociedade Brasileira de Genética2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157146de Freitas, Milena V.; Ariede, Raquel B.; Hata, Milene E.; Mastrochirico Filho, Vito A.; del Pazo, Felipe; et al.; Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 44; 1; 6-2021; 1-61678-46851415-4757CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1590/1678-4685-GMB-2020-0249info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:50Zoai:ri.conicet.gov.ar:11336/157146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:51.142CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
title Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
spellingShingle Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
de Freitas, Milena V.
AQUACULTURE
CONTROL-REGION
GENETIC BREEDING
title_short Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
title_full Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
title_fullStr Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
title_full_unstemmed Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
title_sort Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA
dc.creator.none.fl_str_mv de Freitas, Milena V.
Ariede, Raquel B.
Hata, Milene E.
Mastrochirico Filho, Vito A.
del Pazo, Felipe
Villanova, Gabriela Vanina
Mendonça, Fernando F.
Foresti, Fábio Porto
Hashimoto, Diogo T.
author de Freitas, Milena V.
author_facet de Freitas, Milena V.
Ariede, Raquel B.
Hata, Milene E.
Mastrochirico Filho, Vito A.
del Pazo, Felipe
Villanova, Gabriela Vanina
Mendonça, Fernando F.
Foresti, Fábio Porto
Hashimoto, Diogo T.
author_role author
author2 Ariede, Raquel B.
Hata, Milene E.
Mastrochirico Filho, Vito A.
del Pazo, Felipe
Villanova, Gabriela Vanina
Mendonça, Fernando F.
Foresti, Fábio Porto
Hashimoto, Diogo T.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AQUACULTURE
CONTROL-REGION
GENETIC BREEDING
topic AQUACULTURE
CONTROL-REGION
GENETIC BREEDING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.
Fil: de Freitas, Milena V.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Ariede, Raquel B.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Hata, Milene E.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: Mastrochirico Filho, Vito A.. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia; Brasil
Fil: del Pazo, Felipe. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Villanova, Gabriela Vanina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Mendonça, Fernando F.. Universidade Federal de Sao Paulo; Brasil
Fil: Foresti, Fábio Porto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Hashimoto, Diogo T.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157146
de Freitas, Milena V.; Ariede, Raquel B.; Hata, Milene E.; Mastrochirico Filho, Vito A.; del Pazo, Felipe; et al.; Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 44; 1; 6-2021; 1-6
1678-4685
1415-4757
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157146
identifier_str_mv de Freitas, Milena V.; Ariede, Raquel B.; Hata, Milene E.; Mastrochirico Filho, Vito A.; del Pazo, Felipe; et al.; Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 44; 1; 6-2021; 1-6
1678-4685
1415-4757
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1590/1678-4685-GMB-2020-0249
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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