Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs

Autores
Mastrochirico Filho, Vito Antonio; del Pazo, Felipe; Hata, Milene Elissa; Villanova, Gabriela Vanina; Foresti, Fausto; Vera, Manuel; Martínez, Paulino; Porto Foresti, Fábio; Hashimoto, Diogo Teruo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.
Fil: Mastrochirico Filho, Vito Antonio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: del Pazo, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; Argentina
Fil: Hata, Milene Elissa. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Villanova, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; Argentina
Fil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Vera, Manuel. Universidad de Santiago de Compostela; España
Fil: Martínez, Paulino. Universidad de Santiago de Compostela; España
Fil: Porto Foresti, Fábio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Hashimoto, Diogo Teruo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
AQUACULTURE
BREEDING PROGRAMS
GENETIC VARIABILITY
INBREEDING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150600
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRsMastrochirico Filho, Vito Antoniodel Pazo, FelipeHata, Milene ElissaVillanova, Gabriela VaninaForesti, FaustoVera, ManuelMartínez, PaulinoPorto Foresti, FábioHashimoto, Diogo TeruoAQUACULTUREBREEDING PROGRAMSGENETIC VARIABILITYINBREEDINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.Fil: Mastrochirico Filho, Vito Antonio. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: del Pazo, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; ArgentinaFil: Hata, Milene Elissa. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Villanova, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; ArgentinaFil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Vera, Manuel. Universidad de Santiago de Compostela; EspañaFil: Martínez, Paulino. Universidad de Santiago de Compostela; EspañaFil: Porto Foresti, Fábio. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Hashimoto, Diogo Teruo. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilMDPI2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150600Mastrochirico Filho, Vito Antonio; del Pazo, Felipe; Hata, Milene Elissa; Villanova, Gabriela Vanina; Foresti, Fausto; et al.; Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs; MDPI; Genes; 10; 9; 8-2019; 1-132073-4425CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/10/9/668info:eu-repo/semantics/altIdentifier/doi/10.3390/genes10090668info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:31Zoai:ri.conicet.gov.ar:11336/150600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:31.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
title Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
spellingShingle Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
Mastrochirico Filho, Vito Antonio
AQUACULTURE
BREEDING PROGRAMS
GENETIC VARIABILITY
INBREEDING
title_short Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
title_full Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
title_fullStr Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
title_full_unstemmed Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
title_sort Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs
dc.creator.none.fl_str_mv Mastrochirico Filho, Vito Antonio
del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto Foresti, Fábio
Hashimoto, Diogo Teruo
author Mastrochirico Filho, Vito Antonio
author_facet Mastrochirico Filho, Vito Antonio
del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto Foresti, Fábio
Hashimoto, Diogo Teruo
author_role author
author2 del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto Foresti, Fábio
Hashimoto, Diogo Teruo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AQUACULTURE
BREEDING PROGRAMS
GENETIC VARIABILITY
INBREEDING
topic AQUACULTURE
BREEDING PROGRAMS
GENETIC VARIABILITY
INBREEDING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.
Fil: Mastrochirico Filho, Vito Antonio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: del Pazo, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; Argentina
Fil: Hata, Milene Elissa. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Villanova, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Laboratorio de Biotecnología Acuática.; Argentina
Fil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Vera, Manuel. Universidad de Santiago de Compostela; España
Fil: Martínez, Paulino. Universidad de Santiago de Compostela; España
Fil: Porto Foresti, Fábio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Hashimoto, Diogo Teruo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150600
Mastrochirico Filho, Vito Antonio; del Pazo, Felipe; Hata, Milene Elissa; Villanova, Gabriela Vanina; Foresti, Fausto; et al.; Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs; MDPI; Genes; 10; 9; 8-2019; 1-13
2073-4425
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150600
identifier_str_mv Mastrochirico Filho, Vito Antonio; del Pazo, Felipe; Hata, Milene Elissa; Villanova, Gabriela Vanina; Foresti, Fausto; et al.; Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs; MDPI; Genes; 10; 9; 8-2019; 1-13
2073-4425
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/10/9/668
info:eu-repo/semantics/altIdentifier/doi/10.3390/genes10090668
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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