Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies

Autores
Manavella, Pablo Andrés; Chan, Raquel Lia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
plant transformation
sunflower
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104062
Acceder
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spelling Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studiesManavella, Pablo AndrésChan, Raquel Liaplant transformationsunflowerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaNature Publishing Group2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104062Manavella, Pablo Andrés; Chan, Raquel Lia; Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies; Nature Publishing Group; Nature Protocols; 4; 11; 10-2009; 1699-17071754-2189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/nprot/journal/v4/n11/full/nprot.2009.178.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1038/nprot.2009.178info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:30Zoai:ri.conicet.gov.ar:11336/104062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:30.761CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
title Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
spellingShingle Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
Manavella, Pablo Andrés
plant transformation
sunflower
title_short Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
title_full Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
title_fullStr Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
title_full_unstemmed Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
title_sort Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
dc.creator.none.fl_str_mv Manavella, Pablo Andrés
Chan, Raquel Lia
author Manavella, Pablo Andrés
author_facet Manavella, Pablo Andrés
Chan, Raquel Lia
author_role author
author2 Chan, Raquel Lia
author2_role author
dc.subject.none.fl_str_mv plant transformation
sunflower
topic plant transformation
sunflower
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104062
Manavella, Pablo Andrés; Chan, Raquel Lia; Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies; Nature Publishing Group; Nature Protocols; 4; 11; 10-2009; 1699-1707
1754-2189
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104062
identifier_str_mv Manavella, Pablo Andrés; Chan, Raquel Lia; Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies; Nature Publishing Group; Nature Protocols; 4; 11; 10-2009; 1699-1707
1754-2189
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/nprot/journal/v4/n11/full/nprot.2009.178.html
info:eu-repo/semantics/altIdentifier/doi/10.1038/nprot.2009.178
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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