Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development

Autores
Pinedo, Marcela Lilian; Lechner, L.; Creus, Cecilia Mónica; Simontacchi, Marcela Silvia; Aguirrezábal, Luis Adolfo Nazareno
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. Among them, peroxidases contribute to both loosening and stiffening of the cell wall. They appear to be regulated by various mechanisms, including the action of extracellular inhibitors. To obtain evidence of the role of the enzyme–inhibitor interaction during leaf development, the intercellular washing fluids from Helianthus annuus leaves of different ages were isolated using standard methods of vacuum infiltration and centrifugation. Peroxidase activities, assessed using tetramethylbenzidine as substrate, increased during leaf development, reaching a maximum value after the leaves were fully expanded. An inhibitor, chemically characterised as ascorbate, co-localised with the enzyme in the apoplast. Moreover, there was a strong negative correlation between the action of peroxidase and the micromolar concentration of ascorbate in the apoplastic fluid. The results show that in growing leaves, the in planta ascorbate concentration is able to restrain peroxidase enzyme activity. Then, at the time of growth cessation, the loss of extracellular ascorbate relieves the inhibition on this enzyme that contributes to wall fixation.
Fil: Pinedo, Marcela Lilian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lechner, L.. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Aguirrezábal, Luis Adolfo Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
Ascorbate
Cell Wall
Extracellular
Inhibitor
Leaf Growth
Peroxidase
Sunflower
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23976
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23976
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf developmentPinedo, Marcela LilianLechner, L.Creus, Cecilia MónicaSimontacchi, Marcela SilviaAguirrezábal, Luis Adolfo NazarenoAscorbateCell WallExtracellularInhibitorLeaf GrowthPeroxidaseSunflowerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. Among them, peroxidases contribute to both loosening and stiffening of the cell wall. They appear to be regulated by various mechanisms, including the action of extracellular inhibitors. To obtain evidence of the role of the enzyme–inhibitor interaction during leaf development, the intercellular washing fluids from Helianthus annuus leaves of different ages were isolated using standard methods of vacuum infiltration and centrifugation. Peroxidase activities, assessed using tetramethylbenzidine as substrate, increased during leaf development, reaching a maximum value after the leaves were fully expanded. An inhibitor, chemically characterised as ascorbate, co-localised with the enzyme in the apoplast. Moreover, there was a strong negative correlation between the action of peroxidase and the micromolar concentration of ascorbate in the apoplastic fluid. The results show that in growing leaves, the in planta ascorbate concentration is able to restrain peroxidase enzyme activity. Then, at the time of growth cessation, the loss of extracellular ascorbate relieves the inhibition on this enzyme that contributes to wall fixation.Fil: Pinedo, Marcela Lilian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lechner, L.. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Aguirrezábal, Luis Adolfo Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaWiley Blackwell Publishing, Inc2012-10-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23976Pinedo, Marcela Lilian; Lechner, L.; Creus, Cecilia Mónica; Simontacchi, Marcela Silvia; Aguirrezábal, Luis Adolfo Nazareno; Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 1; 8-10-2012; 60-661435-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2012.00620.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2012.00620.x/abstract?info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:01Zoai:ri.conicet.gov.ar:11336/23976instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:01.516CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
title Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
spellingShingle Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
Pinedo, Marcela Lilian
Ascorbate
Cell Wall
Extracellular
Inhibitor
Leaf Growth
Peroxidase
Sunflower
title_short Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
title_full Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
title_fullStr Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
title_full_unstemmed Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
title_sort Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development
dc.creator.none.fl_str_mv Pinedo, Marcela Lilian
Lechner, L.
Creus, Cecilia Mónica
Simontacchi, Marcela Silvia
Aguirrezábal, Luis Adolfo Nazareno
author Pinedo, Marcela Lilian
author_facet Pinedo, Marcela Lilian
Lechner, L.
Creus, Cecilia Mónica
Simontacchi, Marcela Silvia
Aguirrezábal, Luis Adolfo Nazareno
author_role author
author2 Lechner, L.
Creus, Cecilia Mónica
Simontacchi, Marcela Silvia
Aguirrezábal, Luis Adolfo Nazareno
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ascorbate
Cell Wall
Extracellular
Inhibitor
Leaf Growth
Peroxidase
Sunflower
topic Ascorbate
Cell Wall
Extracellular
Inhibitor
Leaf Growth
Peroxidase
Sunflower
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. Among them, peroxidases contribute to both loosening and stiffening of the cell wall. They appear to be regulated by various mechanisms, including the action of extracellular inhibitors. To obtain evidence of the role of the enzyme–inhibitor interaction during leaf development, the intercellular washing fluids from Helianthus annuus leaves of different ages were isolated using standard methods of vacuum infiltration and centrifugation. Peroxidase activities, assessed using tetramethylbenzidine as substrate, increased during leaf development, reaching a maximum value after the leaves were fully expanded. An inhibitor, chemically characterised as ascorbate, co-localised with the enzyme in the apoplast. Moreover, there was a strong negative correlation between the action of peroxidase and the micromolar concentration of ascorbate in the apoplastic fluid. The results show that in growing leaves, the in planta ascorbate concentration is able to restrain peroxidase enzyme activity. Then, at the time of growth cessation, the loss of extracellular ascorbate relieves the inhibition on this enzyme that contributes to wall fixation.
Fil: Pinedo, Marcela Lilian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lechner, L.. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Simontacchi, Marcela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Aguirrezábal, Luis Adolfo Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description Several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. Among them, peroxidases contribute to both loosening and stiffening of the cell wall. They appear to be regulated by various mechanisms, including the action of extracellular inhibitors. To obtain evidence of the role of the enzyme–inhibitor interaction during leaf development, the intercellular washing fluids from Helianthus annuus leaves of different ages were isolated using standard methods of vacuum infiltration and centrifugation. Peroxidase activities, assessed using tetramethylbenzidine as substrate, increased during leaf development, reaching a maximum value after the leaves were fully expanded. An inhibitor, chemically characterised as ascorbate, co-localised with the enzyme in the apoplast. Moreover, there was a strong negative correlation between the action of peroxidase and the micromolar concentration of ascorbate in the apoplastic fluid. The results show that in growing leaves, the in planta ascorbate concentration is able to restrain peroxidase enzyme activity. Then, at the time of growth cessation, the loss of extracellular ascorbate relieves the inhibition on this enzyme that contributes to wall fixation.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23976
Pinedo, Marcela Lilian; Lechner, L.; Creus, Cecilia Mónica; Simontacchi, Marcela Silvia; Aguirrezábal, Luis Adolfo Nazareno; Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 1; 8-10-2012; 60-66
1435-8603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23976
identifier_str_mv Pinedo, Marcela Lilian; Lechner, L.; Creus, Cecilia Mónica; Simontacchi, Marcela Silvia; Aguirrezábal, Luis Adolfo Nazareno; Endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development; Wiley Blackwell Publishing, Inc; Plant Biology; 15; 1; 8-10-2012; 60-66
1435-8603
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1438-8677.2012.00620.x
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2012.00620.x/abstract?
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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