Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance

Autores
Sheikh Beig Goharrizi, M. A.; Dejahang, A.; Tohidfar, M.; Izadi Darbandi, A.; Carrillo, Nestor Jose; Hajirezaei, M. R.; Vahdati, K.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Somatic embryos of Persian walnut were transformed with two strains of Agrobacterium tumefaciens i.e. LBA4404 and C58, and two plasmids, namely, pBI121 with nptII and gus genes for improving the transformation protocol, and p6u-ubi-FVTI plasmid containing the hpt and fld genes. The transformation frequency was 10%. PCR and RT-PCR analysis proved the presence and expression of the genes. The transgenic and non-transgenic somatic embryos of Persian walnut were exposed to four salinity levels (0, 50, 100, and 200 mM NaCl) and four osmotic stress (0, 1.5, 5, and 10% PEG) levels. After 20 days, the number of survived, secondary and cotyledonary somatic embryos, as well as fresh and dry weights of embryos were evaluated. In addition, the transgenic and non-transgenic regenerated plantlets with 3 leaves and 2.5 cm length were subjected to 200 mM NaCl. In both experiments, the main effects of fld-transformation and stress treatments on evaluated parameters were significant. Transgenic somatic embryos showed no significant differences at 0 and 200 mM NaCl and 0 and 1.5% PEG. Significant differences of transgenic vs. non-transgenic somatic embryos were observed at 50 and 100 mM NaCl and 5 and 10% PEG. Non-transgenic plantlets on medium containing 200 mM NaCl showed complete necrosis and died after 10 days, while transgenic lines continued growth until 45 days. Our results clearly showed that expression of fld gene increased stress tolerance in fld transformant lines of walnut, and also revealed that expression of this specific cyanobacterial protein may provide a powerful tool to improve tolerance to environmental stresses.
Fil: Sheikh Beig Goharrizi, M. A.. Universidad de Teherán; Irán
Fil: Dejahang, A.. Universidad de Teherán; Irán
Fil: Tohidfar, M.. Shahid Beheshti University; Irán
Fil: Izadi Darbandi, A.. Universidad de Teherán; Irán
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, M. R.. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Vahdati, K.. Universidad de Teherán; Irán
Materia
PLANT TRANSFORMATION
AGROBACTERIUM
WALNUT
FLAVODOXIN
SALINITY STRESS TOLERANCE
WALNUT TRANSFORMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52627
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52627
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress ToleranceSheikh Beig Goharrizi, M. A.Dejahang, A.Tohidfar, M.Izadi Darbandi, A.Carrillo, Nestor JoseHajirezaei, M. R.Vahdati, K.PLANT TRANSFORMATIONAGROBACTERIUMWALNUTFLAVODOXINSALINITY STRESS TOLERANCEWALNUT TRANSFORMATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Somatic embryos of Persian walnut were transformed with two strains of Agrobacterium tumefaciens i.e. LBA4404 and C58, and two plasmids, namely, pBI121 with nptII and gus genes for improving the transformation protocol, and p6u-ubi-FVTI plasmid containing the hpt and fld genes. The transformation frequency was 10%. PCR and RT-PCR analysis proved the presence and expression of the genes. The transgenic and non-transgenic somatic embryos of Persian walnut were exposed to four salinity levels (0, 50, 100, and 200 mM NaCl) and four osmotic stress (0, 1.5, 5, and 10% PEG) levels. After 20 days, the number of survived, secondary and cotyledonary somatic embryos, as well as fresh and dry weights of embryos were evaluated. In addition, the transgenic and non-transgenic regenerated plantlets with 3 leaves and 2.5 cm length were subjected to 200 mM NaCl. In both experiments, the main effects of fld-transformation and stress treatments on evaluated parameters were significant. Transgenic somatic embryos showed no significant differences at 0 and 200 mM NaCl and 0 and 1.5% PEG. Significant differences of transgenic vs. non-transgenic somatic embryos were observed at 50 and 100 mM NaCl and 5 and 10% PEG. Non-transgenic plantlets on medium containing 200 mM NaCl showed complete necrosis and died after 10 days, while transgenic lines continued growth until 45 days. Our results clearly showed that expression of fld gene increased stress tolerance in fld transformant lines of walnut, and also revealed that expression of this specific cyanobacterial protein may provide a powerful tool to improve tolerance to environmental stresses.Fil: Sheikh Beig Goharrizi, M. A.. Universidad de Teherán; IránFil: Dejahang, A.. Universidad de Teherán; IránFil: Tohidfar, M.. Shahid Beheshti University; IránFil: Izadi Darbandi, A.. Universidad de Teherán; IránFil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hajirezaei, M. R.. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Vahdati, K.. Universidad de Teherán; IránTarbiat Modares University2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52627Sheikh Beig Goharrizi, M. A.; Dejahang, A.; Tohidfar, M.; Izadi Darbandi, A.; Carrillo, Nestor Jose; et al.; Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance; Tarbiat Modares University; Journal of Agricultural Science and Technology; 18; 2-2016; 423-4351680-7073CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.modares.ac.ir/article-23-5369-en.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:13Zoai:ri.conicet.gov.ar:11336/52627instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:14.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
title Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
spellingShingle Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
Sheikh Beig Goharrizi, M. A.
PLANT TRANSFORMATION
AGROBACTERIUM
WALNUT
FLAVODOXIN
SALINITY STRESS TOLERANCE
WALNUT TRANSFORMATION
title_short Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
title_full Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
title_fullStr Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
title_full_unstemmed Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
title_sort Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
dc.creator.none.fl_str_mv Sheikh Beig Goharrizi, M. A.
Dejahang, A.
Tohidfar, M.
Izadi Darbandi, A.
Carrillo, Nestor Jose
Hajirezaei, M. R.
Vahdati, K.
author Sheikh Beig Goharrizi, M. A.
author_facet Sheikh Beig Goharrizi, M. A.
Dejahang, A.
Tohidfar, M.
Izadi Darbandi, A.
Carrillo, Nestor Jose
Hajirezaei, M. R.
Vahdati, K.
author_role author
author2 Dejahang, A.
Tohidfar, M.
Izadi Darbandi, A.
Carrillo, Nestor Jose
Hajirezaei, M. R.
Vahdati, K.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT TRANSFORMATION
AGROBACTERIUM
WALNUT
FLAVODOXIN
SALINITY STRESS TOLERANCE
WALNUT TRANSFORMATION
topic PLANT TRANSFORMATION
AGROBACTERIUM
WALNUT
FLAVODOXIN
SALINITY STRESS TOLERANCE
WALNUT TRANSFORMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Somatic embryos of Persian walnut were transformed with two strains of Agrobacterium tumefaciens i.e. LBA4404 and C58, and two plasmids, namely, pBI121 with nptII and gus genes for improving the transformation protocol, and p6u-ubi-FVTI plasmid containing the hpt and fld genes. The transformation frequency was 10%. PCR and RT-PCR analysis proved the presence and expression of the genes. The transgenic and non-transgenic somatic embryos of Persian walnut were exposed to four salinity levels (0, 50, 100, and 200 mM NaCl) and four osmotic stress (0, 1.5, 5, and 10% PEG) levels. After 20 days, the number of survived, secondary and cotyledonary somatic embryos, as well as fresh and dry weights of embryos were evaluated. In addition, the transgenic and non-transgenic regenerated plantlets with 3 leaves and 2.5 cm length were subjected to 200 mM NaCl. In both experiments, the main effects of fld-transformation and stress treatments on evaluated parameters were significant. Transgenic somatic embryos showed no significant differences at 0 and 200 mM NaCl and 0 and 1.5% PEG. Significant differences of transgenic vs. non-transgenic somatic embryos were observed at 50 and 100 mM NaCl and 5 and 10% PEG. Non-transgenic plantlets on medium containing 200 mM NaCl showed complete necrosis and died after 10 days, while transgenic lines continued growth until 45 days. Our results clearly showed that expression of fld gene increased stress tolerance in fld transformant lines of walnut, and also revealed that expression of this specific cyanobacterial protein may provide a powerful tool to improve tolerance to environmental stresses.
Fil: Sheikh Beig Goharrizi, M. A.. Universidad de Teherán; Irán
Fil: Dejahang, A.. Universidad de Teherán; Irán
Fil: Tohidfar, M.. Shahid Beheshti University; Irán
Fil: Izadi Darbandi, A.. Universidad de Teherán; Irán
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, M. R.. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Vahdati, K.. Universidad de Teherán; Irán
description Somatic embryos of Persian walnut were transformed with two strains of Agrobacterium tumefaciens i.e. LBA4404 and C58, and two plasmids, namely, pBI121 with nptII and gus genes for improving the transformation protocol, and p6u-ubi-FVTI plasmid containing the hpt and fld genes. The transformation frequency was 10%. PCR and RT-PCR analysis proved the presence and expression of the genes. The transgenic and non-transgenic somatic embryos of Persian walnut were exposed to four salinity levels (0, 50, 100, and 200 mM NaCl) and four osmotic stress (0, 1.5, 5, and 10% PEG) levels. After 20 days, the number of survived, secondary and cotyledonary somatic embryos, as well as fresh and dry weights of embryos were evaluated. In addition, the transgenic and non-transgenic regenerated plantlets with 3 leaves and 2.5 cm length were subjected to 200 mM NaCl. In both experiments, the main effects of fld-transformation and stress treatments on evaluated parameters were significant. Transgenic somatic embryos showed no significant differences at 0 and 200 mM NaCl and 0 and 1.5% PEG. Significant differences of transgenic vs. non-transgenic somatic embryos were observed at 50 and 100 mM NaCl and 5 and 10% PEG. Non-transgenic plantlets on medium containing 200 mM NaCl showed complete necrosis and died after 10 days, while transgenic lines continued growth until 45 days. Our results clearly showed that expression of fld gene increased stress tolerance in fld transformant lines of walnut, and also revealed that expression of this specific cyanobacterial protein may provide a powerful tool to improve tolerance to environmental stresses.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52627
Sheikh Beig Goharrizi, M. A.; Dejahang, A.; Tohidfar, M.; Izadi Darbandi, A.; Carrillo, Nestor Jose; et al.; Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance; Tarbiat Modares University; Journal of Agricultural Science and Technology; 18; 2-2016; 423-435
1680-7073
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52627
identifier_str_mv Sheikh Beig Goharrizi, M. A.; Dejahang, A.; Tohidfar, M.; Izadi Darbandi, A.; Carrillo, Nestor Jose; et al.; Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance; Tarbiat Modares University; Journal of Agricultural Science and Technology; 18; 2-2016; 423-435
1680-7073
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.modares.ac.ir/article-23-5369-en.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Tarbiat Modares University
publisher.none.fl_str_mv Tarbiat Modares University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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