New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins

Autores
Sauka, Diego Herman; Rodriguez, Sonia E.; Benintende, Graciela Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis is an entomopathogenic bacterium characterized by producing parasporal proteinaceous insecticidal crystal inclusions during sporulation. Many strains are capable to also express other insecticidal proteins during the vegetative growing phase that are called Vip. Particularly, Vip3A proteins have activity against certain Lepidoptera species through a unique mechanism of action which emphasized their possible use in resistance management strategies against resistant pests. The aim of the work was to develop a PCR-RFLP method that can distinguish between vip3A genes from B. thuringiensis strains. In addition, four novel vip3Aa genes were cloned and sequenced. The method was originally based on amplification of a single PCR amplicon and the use of two restriction enzymes with recognition sites that facilitate simultaneous detection. Subsequently, a third restriction enzyme was use to distinguished between vip3A variants. Thirteen vip3Aa genes were identified in strains belonging to ten different B. thuringiensis serovars. Three intra-subclass variants of vip3Aa genes could be differentiated. The presented method can serve as an invaluable tool for the investigation of known and novel vip3A genes in B. thuringiensis strains. To the best of our knowledge, it is the first report where variants of a same subclass of insecticidal genes could be distinguished following PCR-RFLP.
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Sonia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
BACILLUS THURINGIENSIS
POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM
SEQUENCING
VIP3A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199525
Acceder
id CONICETDig_d9532d73eb59162eebf3c85baa340986
oai_identifier_str oai:ri.conicet.gov.ar:11336/199525
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteinsSauka, Diego HermanRodriguez, Sonia E.Benintende, Graciela BeatrizBACILLUS THURINGIENSISPOLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISMSEQUENCINGVIP3Ahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus thuringiensis is an entomopathogenic bacterium characterized by producing parasporal proteinaceous insecticidal crystal inclusions during sporulation. Many strains are capable to also express other insecticidal proteins during the vegetative growing phase that are called Vip. Particularly, Vip3A proteins have activity against certain Lepidoptera species through a unique mechanism of action which emphasized their possible use in resistance management strategies against resistant pests. The aim of the work was to develop a PCR-RFLP method that can distinguish between vip3A genes from B. thuringiensis strains. In addition, four novel vip3Aa genes were cloned and sequenced. The method was originally based on amplification of a single PCR amplicon and the use of two restriction enzymes with recognition sites that facilitate simultaneous detection. Subsequently, a third restriction enzyme was use to distinguished between vip3A variants. Thirteen vip3Aa genes were identified in strains belonging to ten different B. thuringiensis serovars. Three intra-subclass variants of vip3Aa genes could be differentiated. The presented method can serve as an invaluable tool for the investigation of known and novel vip3A genes in B. thuringiensis strains. To the best of our knowledge, it is the first report where variants of a same subclass of insecticidal genes could be distinguished following PCR-RFLP.Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Sonia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaKarger2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199525Sauka, Diego Herman; Rodriguez, Sonia E.; Benintende, Graciela Beatriz; New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins; Karger; Journal of Molecular Microbiology and Biotechnology; 22; 6; 3-2013; 373-3801464-1801CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://karger.com/mmb/article-abstract/22/6/373/197209/New-Variants-of-Lepidoptericidal-Toxin-Genes?redirectedFrom=fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1159/000345911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:56:26Zoai:ri.conicet.gov.ar:11336/199525instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:56:26.279CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
title New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
spellingShingle New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
Sauka, Diego Herman
BACILLUS THURINGIENSIS
POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM
SEQUENCING
VIP3A
title_short New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
title_full New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
title_fullStr New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
title_full_unstemmed New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
title_sort New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins
dc.creator.none.fl_str_mv Sauka, Diego Herman
Rodriguez, Sonia E.
Benintende, Graciela Beatriz
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
Rodriguez, Sonia E.
Benintende, Graciela Beatriz
author_role author
author2 Rodriguez, Sonia E.
Benintende, Graciela Beatriz
author2_role author
author
dc.subject.none.fl_str_mv BACILLUS THURINGIENSIS
POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM
SEQUENCING
VIP3A
topic BACILLUS THURINGIENSIS
POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM
SEQUENCING
VIP3A
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus thuringiensis is an entomopathogenic bacterium characterized by producing parasporal proteinaceous insecticidal crystal inclusions during sporulation. Many strains are capable to also express other insecticidal proteins during the vegetative growing phase that are called Vip. Particularly, Vip3A proteins have activity against certain Lepidoptera species through a unique mechanism of action which emphasized their possible use in resistance management strategies against resistant pests. The aim of the work was to develop a PCR-RFLP method that can distinguish between vip3A genes from B. thuringiensis strains. In addition, four novel vip3Aa genes were cloned and sequenced. The method was originally based on amplification of a single PCR amplicon and the use of two restriction enzymes with recognition sites that facilitate simultaneous detection. Subsequently, a third restriction enzyme was use to distinguished between vip3A variants. Thirteen vip3Aa genes were identified in strains belonging to ten different B. thuringiensis serovars. Three intra-subclass variants of vip3Aa genes could be differentiated. The presented method can serve as an invaluable tool for the investigation of known and novel vip3A genes in B. thuringiensis strains. To the best of our knowledge, it is the first report where variants of a same subclass of insecticidal genes could be distinguished following PCR-RFLP.
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Sonia E.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description Bacillus thuringiensis is an entomopathogenic bacterium characterized by producing parasporal proteinaceous insecticidal crystal inclusions during sporulation. Many strains are capable to also express other insecticidal proteins during the vegetative growing phase that are called Vip. Particularly, Vip3A proteins have activity against certain Lepidoptera species through a unique mechanism of action which emphasized their possible use in resistance management strategies against resistant pests. The aim of the work was to develop a PCR-RFLP method that can distinguish between vip3A genes from B. thuringiensis strains. In addition, four novel vip3Aa genes were cloned and sequenced. The method was originally based on amplification of a single PCR amplicon and the use of two restriction enzymes with recognition sites that facilitate simultaneous detection. Subsequently, a third restriction enzyme was use to distinguished between vip3A variants. Thirteen vip3Aa genes were identified in strains belonging to ten different B. thuringiensis serovars. Three intra-subclass variants of vip3Aa genes could be differentiated. The presented method can serve as an invaluable tool for the investigation of known and novel vip3A genes in B. thuringiensis strains. To the best of our knowledge, it is the first report where variants of a same subclass of insecticidal genes could be distinguished following PCR-RFLP.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199525
Sauka, Diego Herman; Rodriguez, Sonia E.; Benintende, Graciela Beatriz; New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins; Karger; Journal of Molecular Microbiology and Biotechnology; 22; 6; 3-2013; 373-380
1464-1801
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199525
identifier_str_mv Sauka, Diego Herman; Rodriguez, Sonia E.; Benintende, Graciela Beatriz; New variants of lepidoptericidal toxin genes encoding bacillus thuringiensis Vip3Aa proteins; Karger; Journal of Molecular Microbiology and Biotechnology; 22; 6; 3-2013; 373-380
1464-1801
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://karger.com/mmb/article-abstract/22/6/373/197209/New-Variants-of-Lepidoptericidal-Toxin-Genes?redirectedFrom=fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1159/000345911
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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