Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis

Autores
Sauka, Diego Herman; Benintende, Graciela Beatriz
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
Bacillus Thuringiensis
Cyt1
Cyt2
Pcr-Rflp
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28884
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensisSauka, Diego HermanBenintende, Graciela BeatrizBacillus ThuringiensisCyt1Cyt2Pcr-Rflphttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaAfrican Journals Online2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28884Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-81607-4106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajol.info/index.php/jtmb/article/view/90994info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:23Zoai:ri.conicet.gov.ar:11336/28884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:23.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
title Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
spellingShingle Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
Sauka, Diego Herman
Bacillus Thuringiensis
Cyt1
Cyt2
Pcr-Rflp
title_short Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
title_full Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
title_fullStr Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
title_full_unstemmed Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
title_sort Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
dc.creator.none.fl_str_mv Sauka, Diego Herman
Benintende, Graciela Beatriz
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
Benintende, Graciela Beatriz
author_role author
author2 Benintende, Graciela Beatriz
author2_role author
dc.subject.none.fl_str_mv Bacillus Thuringiensis
Cyt1
Cyt2
Pcr-Rflp
topic Bacillus Thuringiensis
Cyt1
Cyt2
Pcr-Rflp
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28884
Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-8
1607-4106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28884
identifier_str_mv Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-8
1607-4106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ajol.info/index.php/jtmb/article/view/90994
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv African Journals Online
publisher.none.fl_str_mv African Journals Online
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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