Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis
- Autores
- Sauka, Diego Herman; Benintende, Graciela Beatriz
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina - Materia
-
Bacillus Thuringiensis
Cyt1
Cyt2
Pcr-Rflp - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/28884
Ver los metadatos del registro completo
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Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensisSauka, Diego HermanBenintende, Graciela BeatrizBacillus ThuringiensisCyt1Cyt2Pcr-Rflphttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis.Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaAfrican Journals Online2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28884Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-81607-4106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajol.info/index.php/jtmb/article/view/90994info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:20Zoai:ri.conicet.gov.ar:11336/28884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:21.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
title |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
spellingShingle |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis Sauka, Diego Herman Bacillus Thuringiensis Cyt1 Cyt2 Pcr-Rflp |
title_short |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
title_full |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
title_fullStr |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
title_full_unstemmed |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
title_sort |
Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis |
dc.creator.none.fl_str_mv |
Sauka, Diego Herman Benintende, Graciela Beatriz |
author |
Sauka, Diego Herman |
author_facet |
Sauka, Diego Herman Benintende, Graciela Beatriz |
author_role |
author |
author2 |
Benintende, Graciela Beatriz |
author2_role |
author |
dc.subject.none.fl_str_mv |
Bacillus Thuringiensis Cyt1 Cyt2 Pcr-Rflp |
topic |
Bacillus Thuringiensis Cyt1 Cyt2 Pcr-Rflp |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis. Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronomicas. Instituto de Microbiología y Zoología Agrícola; Argentina |
description |
Polymerase chain reaction-restriction fragment length polymorphism methods for identification of cyt subclasses from Bacillus thuringiensis were established. Eight of 68 standard and ten of 107 Argentine B. thuringiensis strains harbor at least one cyt gene. The combination of cyt1Aa/cyt2Ba genes was identified in four standard and in ten native strains, whereas the cyt1Ba, cyt2Aa and cyt2Bb genes alone and the cyt1Ab/cyt2Bc genes together were found in four different standard strains respectively. The cyt2Ba genes from three B. thuringiensis svar israelensis strains and two Argentine B. thuringiensis strains were cloned, sequenced and designated as cyt2Ba10 to cyt2Ba14 respectively. These results suggest that these methods are relevant tools for identification of toxin genes encoding Cyt proteins in B. thuringiensis. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/28884 Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-8 1607-4106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/28884 |
identifier_str_mv |
Sauka, Diego Herman; Benintende, Graciela Beatriz; Identification of toxin genes encoding Cyt proteins from standard and Argentine strains of Bacillus thuringiensis; African Journals Online; Journal of Tropical Microbiology and Biotechnology; 8; 1; -1-2012; 3-8 1607-4106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ajol.info/index.php/jtmb/article/view/90994 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
African Journals Online |
publisher.none.fl_str_mv |
African Journals Online |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613100768067584 |
score |
13.070432 |