Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features

Autores
Aldana, Ana Agustina; Malatto, Laura; Rehman, M. A. U.; Boccaccini, A. R.; Abraham, Gustavo Abel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.
Fil: Aldana, Ana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Malatto, Laura. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Rehman, M. A. U.. Universitat Erlangen-Nuremberg; Alemania
Fil: Boccaccini, A. R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
GELATIN
ELECTROSPINNING
TISSUE ENGINEERING
NANOFABRICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/119231
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological featuresAldana, Ana AgustinaMalatto, LauraRehman, M. A. U.Boccaccini, A. R.Abraham, Gustavo AbelGELATINELECTROSPINNINGTISSUE ENGINEERINGNANOFABRICATIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.Fil: Aldana, Ana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Malatto, Laura. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Rehman, M. A. U.. Universitat Erlangen-Nuremberg; AlemaniaFil: Boccaccini, A. R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaMDPI2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119231Aldana, Ana Agustina; Malatto, Laura; Rehman, M. A. U.; Boccaccini, A. R.; Abraham, Gustavo Abel; Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features; MDPI; Nanomaterials; 9; 1; 1-2019; 120-1322079-4991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-4991/9/1/120info:eu-repo/semantics/altIdentifier/doi/10.3390/nano9010120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:13Zoai:ri.conicet.gov.ar:11336/119231instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:13.266CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
title Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
spellingShingle Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
Aldana, Ana Agustina
GELATIN
ELECTROSPINNING
TISSUE ENGINEERING
NANOFABRICATION
title_short Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
title_full Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
title_fullStr Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
title_full_unstemmed Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
title_sort Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features
dc.creator.none.fl_str_mv Aldana, Ana Agustina
Malatto, Laura
Rehman, M. A. U.
Boccaccini, A. R.
Abraham, Gustavo Abel
author Aldana, Ana Agustina
author_facet Aldana, Ana Agustina
Malatto, Laura
Rehman, M. A. U.
Boccaccini, A. R.
Abraham, Gustavo Abel
author_role author
author2 Malatto, Laura
Rehman, M. A. U.
Boccaccini, A. R.
Abraham, Gustavo Abel
author2_role author
author
author
author
dc.subject.none.fl_str_mv GELATIN
ELECTROSPINNING
TISSUE ENGINEERING
NANOFABRICATION
topic GELATIN
ELECTROSPINNING
TISSUE ENGINEERING
NANOFABRICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.
Fil: Aldana, Ana Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Malatto, Laura. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Rehman, M. A. U.. Universitat Erlangen-Nuremberg; Alemania
Fil: Boccaccini, A. R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description The design of biomimetic biomaterials for cell culture has become a great tool to study and understand cell behavior, tissue degradation, and lesion. Topographical and morphological features play an important role in modulating cell behavior. In this study, a dual methodology was evaluated to generate novel gelatin methacrylate (GelMA)-based scaffolds with nano and micro topographical and morphological features. First, electrospinning parameters and crosslinking processes were optimized to obtain electrospun nanofibrous scaffolds. GelMA mats were characterized by SEM, FTIR, DSC, TGA, contact angle, and water uptake. Various nanofibrous GelMA mats with defect-free fibers and stability in aqueous media were obtained. Then, micropatterned molds produced by photolithography were used as collectors in the electrospinning process. Thus, biocompatible GelMA nanofibrous scaffolds with micro-patterns that mimic extracellular matrix were obtained successfully by combining two micro/nanofabrication techniques, electrospinning, and micromolding. Taking into account the cell viability results, the methodology used in this study could be considered a valuable tool to develop patterned GelMA based nanofibrous scaffolds for cell culture and tissue engineering.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119231
Aldana, Ana Agustina; Malatto, Laura; Rehman, M. A. U.; Boccaccini, A. R.; Abraham, Gustavo Abel; Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features; MDPI; Nanomaterials; 9; 1; 1-2019; 120-132
2079-4991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119231
identifier_str_mv Aldana, Ana Agustina; Malatto, Laura; Rehman, M. A. U.; Boccaccini, A. R.; Abraham, Gustavo Abel; Fabrication of gelatin methacrylate (GelMA) scaffolds with nano- and micro-topographical and morphological features; MDPI; Nanomaterials; 9; 1; 1-2019; 120-132
2079-4991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-4991/9/1/120
info:eu-repo/semantics/altIdentifier/doi/10.3390/nano9010120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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