Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia

Autores
Ágredo-Campos, Ángela Sofía; Camussone, Cecilia María; Akineden, Ömer; Fernández Silva, Jorge A.; Ramírez Vásquez, Nicolás F.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia; Colombia
Fil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Akineden, Ömer. Justus Liebig Universitat Giessen.; Alemania
Fil: Fernández Silva, Jorge A.. Universidad de Antioquia; Colombia
Fil: Ramírez Vásquez, Nicolás F.. Universidad de Antioquia; Colombia
Materia
Bovine mastitis
Antimicrobial resistance
MRSA
Staphylococcal enterotoxins
PFGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281714
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in ColombiaÁgredo-Campos, Ángela SofíaCamussone, Cecilia MaríaAkineden, ÖmerFernández Silva, Jorge A.Ramírez Vásquez, Nicolás F.Bovine mastitisAntimicrobial resistanceMRSAStaphylococcal enterotoxinsPFGEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia; ColombiaFil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Akineden, Ömer. Justus Liebig Universitat Giessen.; AlemaniaFil: Fernández Silva, Jorge A.. Universidad de Antioquia; ColombiaFil: Ramírez Vásquez, Nicolás F.. Universidad de Antioquia; ColombiaElsevier2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281714Ágredo-Campos, Ángela Sofía; Camussone, Cecilia María; Akineden, Ömer; Fernández Silva, Jorge A.; Ramírez Vásquez, Nicolás F.; Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia; Elsevier; International Dairy Journal; 160; 1-2025; 1-80958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0958694624002085info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2024.106088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:22:41Zoai:ri.conicet.gov.ar:11336/281714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:22:41.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
spellingShingle Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
Ágredo-Campos, Ángela Sofía
Bovine mastitis
Antimicrobial resistance
MRSA
Staphylococcal enterotoxins
PFGE
title_short Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_full Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_fullStr Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_full_unstemmed Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_sort Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
dc.creator.none.fl_str_mv Ágredo-Campos, Ángela Sofía
Camussone, Cecilia María
Akineden, Ömer
Fernández Silva, Jorge A.
Ramírez Vásquez, Nicolás F.
author Ágredo-Campos, Ángela Sofía
author_facet Ágredo-Campos, Ángela Sofía
Camussone, Cecilia María
Akineden, Ömer
Fernández Silva, Jorge A.
Ramírez Vásquez, Nicolás F.
author_role author
author2 Camussone, Cecilia María
Akineden, Ömer
Fernández Silva, Jorge A.
Ramírez Vásquez, Nicolás F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine mastitis
Antimicrobial resistance
MRSA
Staphylococcal enterotoxins
PFGE
topic Bovine mastitis
Antimicrobial resistance
MRSA
Staphylococcal enterotoxins
PFGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia; Colombia
Fil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Akineden, Ömer. Justus Liebig Universitat Giessen.; Alemania
Fil: Fernández Silva, Jorge A.. Universidad de Antioquia; Colombia
Fil: Ramírez Vásquez, Nicolás F.. Universidad de Antioquia; Colombia
description Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281714
Ágredo-Campos, Ángela Sofía; Camussone, Cecilia María; Akineden, Ömer; Fernández Silva, Jorge A.; Ramírez Vásquez, Nicolás F.; Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia; Elsevier; International Dairy Journal; 160; 1-2025; 1-8
0958-6946
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281714
identifier_str_mv Ágredo-Campos, Ángela Sofía; Camussone, Cecilia María; Akineden, Ömer; Fernández Silva, Jorge A.; Ramírez Vásquez, Nicolás F.; Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia; Elsevier; International Dairy Journal; 160; 1-2025; 1-8
0958-6946
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0958694624002085
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2024.106088
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.822162

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