Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms

Autores
Lasagno, Mirta Cristina; Reinoso, Elina Beatríz; Dieser, Silvana Andrea; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana; Bogni, Cristina Ines; Odierno, Liliana Mónica
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare not associated with the virulence profiles.
Fil: Lasagno, Mirta Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Bogni, Cristina Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
STREP. UBERIS
BOVINA SUBCLINICAL MASTITIS
PFGE
VIRULENCE FACTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195503

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spelling Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farmsLasagno, Mirta CristinaReinoso, Elina BeatrízDieser, Silvana AndreaCalvinho, Luis FernandoBuzzola, Fernanda RoxanaBogni, Cristina InesOdierno, Liliana MónicaSTREP. UBERISBOVINA SUBCLINICAL MASTITISPFGEVIRULENCE FACTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare not associated with the virulence profiles.Fil: Lasagno, Mirta Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Bogni, Cristina Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaAsociación Argentina de Microbiología2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195503Lasagno, Mirta Cristina; Reinoso, Elina Beatríz; Dieser, Silvana Andrea; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana; et al.; Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 43; 3; 7-2011; 212-2170325-75411851-7617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/www.scielo.org.ar/scielo.php?pid=0325-7541&script=sci_serialinfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0325-75412011000300009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:03Zoai:ri.conicet.gov.ar:11336/195503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:03.407CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
title Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
spellingShingle Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
Lasagno, Mirta Cristina
STREP. UBERIS
BOVINA SUBCLINICAL MASTITIS
PFGE
VIRULENCE FACTORS
title_short Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
title_full Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
title_fullStr Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
title_full_unstemmed Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
title_sort Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms
dc.creator.none.fl_str_mv Lasagno, Mirta Cristina
Reinoso, Elina Beatríz
Dieser, Silvana Andrea
Calvinho, Luis Fernando
Buzzola, Fernanda Roxana
Bogni, Cristina Ines
Odierno, Liliana Mónica
author Lasagno, Mirta Cristina
author_facet Lasagno, Mirta Cristina
Reinoso, Elina Beatríz
Dieser, Silvana Andrea
Calvinho, Luis Fernando
Buzzola, Fernanda Roxana
Bogni, Cristina Ines
Odierno, Liliana Mónica
author_role author
author2 Reinoso, Elina Beatríz
Dieser, Silvana Andrea
Calvinho, Luis Fernando
Buzzola, Fernanda Roxana
Bogni, Cristina Ines
Odierno, Liliana Mónica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv STREP. UBERIS
BOVINA SUBCLINICAL MASTITIS
PFGE
VIRULENCE FACTORS
topic STREP. UBERIS
BOVINA SUBCLINICAL MASTITIS
PFGE
VIRULENCE FACTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare not associated with the virulence profiles.
Fil: Lasagno, Mirta Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Bogni, Cristina Ines. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberiswere isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. TheS. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profileswere identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similaritywere identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberisstrains with the same PFGE pattern showed different virulence profiles. Bovine S. uberisstrains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypiccharacteristics, and the PFGE patternsare not associated with the virulence profiles.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195503
Lasagno, Mirta Cristina; Reinoso, Elina Beatríz; Dieser, Silvana Andrea; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana; et al.; Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 43; 3; 7-2011; 212-217
0325-7541
1851-7617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195503
identifier_str_mv Lasagno, Mirta Cristina; Reinoso, Elina Beatríz; Dieser, Silvana Andrea; Calvinho, Luis Fernando; Buzzola, Fernanda Roxana; et al.; Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 43; 3; 7-2011; 212-217
0325-7541
1851-7617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1590/S0325-75412011000300009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
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instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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