Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy

Autores
Velez, Eva Maria del Mar; Maldonado, Natalia Cecilia; Carmuega, E.; Weil, R.; Perdigon, Gabriela del Valle
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intestinal microbiota has an essential role in the maturation and function of the immune system. Changes in the microbiota, together withgenetic factors induce the development of allergy with a typical Th2 profile(1). Several studies have shown that probiotics have a beneficialeffect in disease and can stimulate the mucosal immune system by improving microbiota composition(2); hence, probiotics oral admin-istration is proposed to avoid the allergy development by balancing microbiota and modulating the immune system, not only at theintestinal mucosa, but also in other mucosal sites and at systemic level(3). This study was aimed to evaluate the adjuvant immune effect ofthe oral administration of a probiotic fermented milk (PFM) in the intestinal mucosa and the extent of these effects on distant mucosalsites (bronchus) in an experimental mouse model of allergic airway reactivity to ovoalbumin (OVA). Experimental groups: normal-control(NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P) (5d-PFM +OVA +H2O) and Continuous (C) (5d-PFM +OVA +PFM). SC, P and C were sensitized with OVA 1 % followed by daily exposures to OVA aerosols. At 7 and 15 days post-sensitization (dPS) specific-IgE, specific-IgG and IL-10 in serum, total S-IgA, IL-10 and IFN-gin intestinal fluid (IF), specific-IgE inbronchoalveolar lavage (BAL) were determined by ELISA. The number of IgA +, IL-10 +and IL-4 +cells in the lamina propria of smallintestine (SI) and lungs were determined by immunofluorescense assay. In large intestine (LI) total populations of total anaerobes,lactobacilli, bifidobacteria and enterobacteria in selective media were determined. Specific-IgE was reduced in treated groups with regardto SC in serum and BAL (DO 450nm) (Table 1). Specific-IgG increased in all groups but showed the highest values in C (Table 2).Table 1. Anti OVA IgE in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.08°0.01 0.09°0.017 dPS 0.87°0.07 0.38°0.13 0.38°0.01215 dPS 0.47°0.02 0.47°0.01 0.31°0.06Table 2. Anti OVA IgG in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.06°0.01 0.08°0.037 dPS 0.78°0.01 0.80°0.05 1,03°0.0715 dPS 0.67°0.06 0.65°0.13 0.92°0.10IL-10 values increased in SC in SI fluid compared to treated groups, with no changes in serum. Total S-IgA from SI fluid significantlyincreased in CS and P compared to NC, B and C. IL-4 +cells increased significantly in SC in lungs compared to the other groups at 7 and15 dPS; in SI no differences were found between controls and treated groups. The number of IL-10 +cells was significantly higher in thelamina propria of SI from SC group compared to P and C at 7 and 15 dPS. PFM induced a decrease of enterobacteria and increase ofbifidobacteria only in C, other populations were not affected. PFM reduced specific-IgE levels in serum and BAL in mice with continuoustreatment; this could be mediated by intestinal microbiota regulation that might have influence on the expression of cytokines IL-4(Th2 type) and IL-10 (regulatory cytokine) at the mucosal level (intestine and lungs). However systemic and mucosal normal immuneresponse was not affected because IgG and S-IgA production was not altered by IL-10.1. Bjorksten B, Sepp E, Julge K et al. (2001) J Allergy Clin Immunol 108, 516?520.2. Galdeano CM & Perdigon G (2006) Clin Vaccine Immunol 13, 219?226.3. de Moreno de LeBlanc A, Maldonado Galdeano C, Chaves, S and Perdigon, G. (2005) Eur J Inflamm 3, 25?30.
Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, E.. Nutritia; Argentina
Fil: Weil, R.. DANONE; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Materia
Probioticos
Sistema Inmune
Alergia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70944

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oai_identifier_str oai:ri.conicet.gov.ar:11336/70944
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergyVelez, Eva Maria del MarMaldonado, Natalia CeciliaCarmuega, E.Weil, R.Perdigon, Gabriela del ValleProbioticosSistema InmuneAlergiahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Intestinal microbiota has an essential role in the maturation and function of the immune system. Changes in the microbiota, together withgenetic factors induce the development of allergy with a typical Th2 profile(1). Several studies have shown that probiotics have a beneficialeffect in disease and can stimulate the mucosal immune system by improving microbiota composition(2); hence, probiotics oral admin-istration is proposed to avoid the allergy development by balancing microbiota and modulating the immune system, not only at theintestinal mucosa, but also in other mucosal sites and at systemic level(3). This study was aimed to evaluate the adjuvant immune effect ofthe oral administration of a probiotic fermented milk (PFM) in the intestinal mucosa and the extent of these effects on distant mucosalsites (bronchus) in an experimental mouse model of allergic airway reactivity to ovoalbumin (OVA). Experimental groups: normal-control(NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P) (5d-PFM +OVA +H2O) and Continuous (C) (5d-PFM +OVA +PFM). SC, P and C were sensitized with OVA 1 % followed by daily exposures to OVA aerosols. At 7 and 15 days post-sensitization (dPS) specific-IgE, specific-IgG and IL-10 in serum, total S-IgA, IL-10 and IFN-gin intestinal fluid (IF), specific-IgE inbronchoalveolar lavage (BAL) were determined by ELISA. The number of IgA +, IL-10 +and IL-4 +cells in the lamina propria of smallintestine (SI) and lungs were determined by immunofluorescense assay. In large intestine (LI) total populations of total anaerobes,lactobacilli, bifidobacteria and enterobacteria in selective media were determined. Specific-IgE was reduced in treated groups with regardto SC in serum and BAL (DO 450nm) (Table 1). Specific-IgG increased in all groups but showed the highest values in C (Table 2).Table 1. Anti OVA IgE in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.08°0.01 0.09°0.017 dPS 0.87°0.07 0.38°0.13 0.38°0.01215 dPS 0.47°0.02 0.47°0.01 0.31°0.06Table 2. Anti OVA IgG in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.06°0.01 0.08°0.037 dPS 0.78°0.01 0.80°0.05 1,03°0.0715 dPS 0.67°0.06 0.65°0.13 0.92°0.10IL-10 values increased in SC in SI fluid compared to treated groups, with no changes in serum. Total S-IgA from SI fluid significantlyincreased in CS and P compared to NC, B and C. IL-4 +cells increased significantly in SC in lungs compared to the other groups at 7 and15 dPS; in SI no differences were found between controls and treated groups. The number of IL-10 +cells was significantly higher in thelamina propria of SI from SC group compared to P and C at 7 and 15 dPS. PFM induced a decrease of enterobacteria and increase ofbifidobacteria only in C, other populations were not affected. PFM reduced specific-IgE levels in serum and BAL in mice with continuoustreatment; this could be mediated by intestinal microbiota regulation that might have influence on the expression of cytokines IL-4(Th2 type) and IL-10 (regulatory cytokine) at the mucosal level (intestine and lungs). However systemic and mucosal normal immuneresponse was not affected because IgG and S-IgA production was not altered by IL-10.1. Bjorksten B, Sepp E, Julge K et al. (2001) J Allergy Clin Immunol 108, 516?520.2. Galdeano CM & Perdigon G (2006) Clin Vaccine Immunol 13, 219?226.3. de Moreno de LeBlanc A, Maldonado Galdeano C, Chaves, S and Perdigon, G. (2005) Eur J Inflamm 3, 25?30.Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Carmuega, E.. Nutritia; ArgentinaFil: Weil, R.. DANONE; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaCambridge University Press2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70944Velez, Eva Maria del Mar; Maldonado, Natalia Cecilia; Carmuega, E.; Weil, R.; Perdigon, Gabriela del Valle; Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy; Cambridge University Press; Proceedings Of The Nutrition Society; 72; OCE1; 4-20130029-6651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journals.cambridge.org/abstract_S002966511300058Xinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S002966511300058Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:07Zoai:ri.conicet.gov.ar:11336/70944instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:07.977CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
title Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
spellingShingle Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
Velez, Eva Maria del Mar
Probioticos
Sistema Inmune
Alergia
title_short Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
title_full Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
title_fullStr Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
title_full_unstemmed Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
title_sort Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy
dc.creator.none.fl_str_mv Velez, Eva Maria del Mar
Maldonado, Natalia Cecilia
Carmuega, E.
Weil, R.
Perdigon, Gabriela del Valle
author Velez, Eva Maria del Mar
author_facet Velez, Eva Maria del Mar
Maldonado, Natalia Cecilia
Carmuega, E.
Weil, R.
Perdigon, Gabriela del Valle
author_role author
author2 Maldonado, Natalia Cecilia
Carmuega, E.
Weil, R.
Perdigon, Gabriela del Valle
author2_role author
author
author
author
dc.subject.none.fl_str_mv Probioticos
Sistema Inmune
Alergia
topic Probioticos
Sistema Inmune
Alergia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Intestinal microbiota has an essential role in the maturation and function of the immune system. Changes in the microbiota, together withgenetic factors induce the development of allergy with a typical Th2 profile(1). Several studies have shown that probiotics have a beneficialeffect in disease and can stimulate the mucosal immune system by improving microbiota composition(2); hence, probiotics oral admin-istration is proposed to avoid the allergy development by balancing microbiota and modulating the immune system, not only at theintestinal mucosa, but also in other mucosal sites and at systemic level(3). This study was aimed to evaluate the adjuvant immune effect ofthe oral administration of a probiotic fermented milk (PFM) in the intestinal mucosa and the extent of these effects on distant mucosalsites (bronchus) in an experimental mouse model of allergic airway reactivity to ovoalbumin (OVA). Experimental groups: normal-control(NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P) (5d-PFM +OVA +H2O) and Continuous (C) (5d-PFM +OVA +PFM). SC, P and C were sensitized with OVA 1 % followed by daily exposures to OVA aerosols. At 7 and 15 days post-sensitization (dPS) specific-IgE, specific-IgG and IL-10 in serum, total S-IgA, IL-10 and IFN-gin intestinal fluid (IF), specific-IgE inbronchoalveolar lavage (BAL) were determined by ELISA. The number of IgA +, IL-10 +and IL-4 +cells in the lamina propria of smallintestine (SI) and lungs were determined by immunofluorescense assay. In large intestine (LI) total populations of total anaerobes,lactobacilli, bifidobacteria and enterobacteria in selective media were determined. Specific-IgE was reduced in treated groups with regardto SC in serum and BAL (DO 450nm) (Table 1). Specific-IgG increased in all groups but showed the highest values in C (Table 2).Table 1. Anti OVA IgE in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.08°0.01 0.09°0.017 dPS 0.87°0.07 0.38°0.13 0.38°0.01215 dPS 0.47°0.02 0.47°0.01 0.31°0.06Table 2. Anti OVA IgG in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.06°0.01 0.08°0.037 dPS 0.78°0.01 0.80°0.05 1,03°0.0715 dPS 0.67°0.06 0.65°0.13 0.92°0.10IL-10 values increased in SC in SI fluid compared to treated groups, with no changes in serum. Total S-IgA from SI fluid significantlyincreased in CS and P compared to NC, B and C. IL-4 +cells increased significantly in SC in lungs compared to the other groups at 7 and15 dPS; in SI no differences were found between controls and treated groups. The number of IL-10 +cells was significantly higher in thelamina propria of SI from SC group compared to P and C at 7 and 15 dPS. PFM induced a decrease of enterobacteria and increase ofbifidobacteria only in C, other populations were not affected. PFM reduced specific-IgE levels in serum and BAL in mice with continuoustreatment; this could be mediated by intestinal microbiota regulation that might have influence on the expression of cytokines IL-4(Th2 type) and IL-10 (regulatory cytokine) at the mucosal level (intestine and lungs). However systemic and mucosal normal immuneresponse was not affected because IgG and S-IgA production was not altered by IL-10.1. Bjorksten B, Sepp E, Julge K et al. (2001) J Allergy Clin Immunol 108, 516?520.2. Galdeano CM & Perdigon G (2006) Clin Vaccine Immunol 13, 219?226.3. de Moreno de LeBlanc A, Maldonado Galdeano C, Chaves, S and Perdigon, G. (2005) Eur J Inflamm 3, 25?30.
Fil: Velez, Eva Maria del Mar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, E.. Nutritia; Argentina
Fil: Weil, R.. DANONE; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
description Intestinal microbiota has an essential role in the maturation and function of the immune system. Changes in the microbiota, together withgenetic factors induce the development of allergy with a typical Th2 profile(1). Several studies have shown that probiotics have a beneficialeffect in disease and can stimulate the mucosal immune system by improving microbiota composition(2); hence, probiotics oral admin-istration is proposed to avoid the allergy development by balancing microbiota and modulating the immune system, not only at theintestinal mucosa, but also in other mucosal sites and at systemic level(3). This study was aimed to evaluate the adjuvant immune effect ofthe oral administration of a probiotic fermented milk (PFM) in the intestinal mucosa and the extent of these effects on distant mucosalsites (bronchus) in an experimental mouse model of allergic airway reactivity to ovoalbumin (OVA). Experimental groups: normal-control(NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P) (5d-PFM +OVA +H2O) and Continuous (C) (5d-PFM +OVA +PFM). SC, P and C were sensitized with OVA 1 % followed by daily exposures to OVA aerosols. At 7 and 15 days post-sensitization (dPS) specific-IgE, specific-IgG and IL-10 in serum, total S-IgA, IL-10 and IFN-gin intestinal fluid (IF), specific-IgE inbronchoalveolar lavage (BAL) were determined by ELISA. The number of IgA +, IL-10 +and IL-4 +cells in the lamina propria of smallintestine (SI) and lungs were determined by immunofluorescense assay. In large intestine (LI) total populations of total anaerobes,lactobacilli, bifidobacteria and enterobacteria in selective media were determined. Specific-IgE was reduced in treated groups with regardto SC in serum and BAL (DO 450nm) (Table 1). Specific-IgG increased in all groups but showed the highest values in C (Table 2).Table 1. Anti OVA IgE in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.08°0.01 0.09°0.017 dPS 0.87°0.07 0.38°0.13 0.38°0.01215 dPS 0.47°0.02 0.47°0.01 0.31°0.06Table 2. Anti OVA IgG in serum (Absorbance, DO 450 nm) (media°SD)NC B SC P C5 dPFM 0.06°0.01 0.08°0.037 dPS 0.78°0.01 0.80°0.05 1,03°0.0715 dPS 0.67°0.06 0.65°0.13 0.92°0.10IL-10 values increased in SC in SI fluid compared to treated groups, with no changes in serum. Total S-IgA from SI fluid significantlyincreased in CS and P compared to NC, B and C. IL-4 +cells increased significantly in SC in lungs compared to the other groups at 7 and15 dPS; in SI no differences were found between controls and treated groups. The number of IL-10 +cells was significantly higher in thelamina propria of SI from SC group compared to P and C at 7 and 15 dPS. PFM induced a decrease of enterobacteria and increase ofbifidobacteria only in C, other populations were not affected. PFM reduced specific-IgE levels in serum and BAL in mice with continuoustreatment; this could be mediated by intestinal microbiota regulation that might have influence on the expression of cytokines IL-4(Th2 type) and IL-10 (regulatory cytokine) at the mucosal level (intestine and lungs). However systemic and mucosal normal immuneresponse was not affected because IgG and S-IgA production was not altered by IL-10.1. Bjorksten B, Sepp E, Julge K et al. (2001) J Allergy Clin Immunol 108, 516?520.2. Galdeano CM & Perdigon G (2006) Clin Vaccine Immunol 13, 219?226.3. de Moreno de LeBlanc A, Maldonado Galdeano C, Chaves, S and Perdigon, G. (2005) Eur J Inflamm 3, 25?30.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70944
Velez, Eva Maria del Mar; Maldonado, Natalia Cecilia; Carmuega, E.; Weil, R.; Perdigon, Gabriela del Valle; Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy; Cambridge University Press; Proceedings Of The Nutrition Society; 72; OCE1; 4-2013
0029-6651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70944
identifier_str_mv Velez, Eva Maria del Mar; Maldonado, Natalia Cecilia; Carmuega, E.; Weil, R.; Perdigon, Gabriela del Valle; Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy; Cambridge University Press; Proceedings Of The Nutrition Society; 72; OCE1; 4-2013
0029-6651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1017/S002966511300058X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
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