Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters

Autores
Zapata Zapata, Arley D.; Voget, Claudio Enrique
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study reports the use of borosilicate glass fiber microfilters (GFMs) for capturing and concentrating Geotrichum klebahnii polygalacturonase (GkPG) from the culture media through a simple coupled filtration-sorption process. GFM was characterized by scanning electron microscopy and intrusion mercury porosimetry. Adsorption of GkPG was almost instantaneous with maximum effectiveness at pH 3.0. Using the parameters of Langmuir model and a material balance, a filter bed consisting of a stack of GFM was designed for recovering GkPG from a certain volume of culture media. After adsorption and washing, the enzyme was efficiently eluted with a buffer solution at pH 5.0 containing 0.5 M NaCl. The filter bed was reused in several sorption cycles and enzyme recovery could be also done without prior cell removal with the benefits of using microfiltration for enzyme cell separation in a single step. Under the process conditions employed, GkPG recovery was about 80% with a concentration factor of about 4-fold.
Fil: Zapata Zapata, Arley D.. Universidad Nacional de Colombia; Colombia
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
Borosilicate Glass Fiber Microfilters
Enzyme Capturing
G. Klebahnii Polygalacturonase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82932
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfiltersZapata Zapata, Arley D.Voget, Claudio EnriqueBorosilicate Glass Fiber MicrofiltersEnzyme CapturingG. Klebahnii Polygalacturonasehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This study reports the use of borosilicate glass fiber microfilters (GFMs) for capturing and concentrating Geotrichum klebahnii polygalacturonase (GkPG) from the culture media through a simple coupled filtration-sorption process. GFM was characterized by scanning electron microscopy and intrusion mercury porosimetry. Adsorption of GkPG was almost instantaneous with maximum effectiveness at pH 3.0. Using the parameters of Langmuir model and a material balance, a filter bed consisting of a stack of GFM was designed for recovering GkPG from a certain volume of culture media. After adsorption and washing, the enzyme was efficiently eluted with a buffer solution at pH 5.0 containing 0.5 M NaCl. The filter bed was reused in several sorption cycles and enzyme recovery could be also done without prior cell removal with the benefits of using microfiltration for enzyme cell separation in a single step. Under the process conditions employed, GkPG recovery was about 80% with a concentration factor of about 4-fold.Fil: Zapata Zapata, Arley D.. Universidad Nacional de Colombia; ColombiaFil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82932Zapata Zapata, Arley D.; Voget, Claudio Enrique; Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters; Elsevier; Process Biochemistry; 47; 8; 8-2012; 1277-12811359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511312001742info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2012.04.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:23Zoai:ri.conicet.gov.ar:11336/82932instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:24.039CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
title Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
spellingShingle Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
Zapata Zapata, Arley D.
Borosilicate Glass Fiber Microfilters
Enzyme Capturing
G. Klebahnii Polygalacturonase
title_short Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
title_full Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
title_fullStr Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
title_full_unstemmed Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
title_sort Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters
dc.creator.none.fl_str_mv Zapata Zapata, Arley D.
Voget, Claudio Enrique
author Zapata Zapata, Arley D.
author_facet Zapata Zapata, Arley D.
Voget, Claudio Enrique
author_role author
author2 Voget, Claudio Enrique
author2_role author
dc.subject.none.fl_str_mv Borosilicate Glass Fiber Microfilters
Enzyme Capturing
G. Klebahnii Polygalacturonase
topic Borosilicate Glass Fiber Microfilters
Enzyme Capturing
G. Klebahnii Polygalacturonase
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This study reports the use of borosilicate glass fiber microfilters (GFMs) for capturing and concentrating Geotrichum klebahnii polygalacturonase (GkPG) from the culture media through a simple coupled filtration-sorption process. GFM was characterized by scanning electron microscopy and intrusion mercury porosimetry. Adsorption of GkPG was almost instantaneous with maximum effectiveness at pH 3.0. Using the parameters of Langmuir model and a material balance, a filter bed consisting of a stack of GFM was designed for recovering GkPG from a certain volume of culture media. After adsorption and washing, the enzyme was efficiently eluted with a buffer solution at pH 5.0 containing 0.5 M NaCl. The filter bed was reused in several sorption cycles and enzyme recovery could be also done without prior cell removal with the benefits of using microfiltration for enzyme cell separation in a single step. Under the process conditions employed, GkPG recovery was about 80% with a concentration factor of about 4-fold.
Fil: Zapata Zapata, Arley D.. Universidad Nacional de Colombia; Colombia
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description This study reports the use of borosilicate glass fiber microfilters (GFMs) for capturing and concentrating Geotrichum klebahnii polygalacturonase (GkPG) from the culture media through a simple coupled filtration-sorption process. GFM was characterized by scanning electron microscopy and intrusion mercury porosimetry. Adsorption of GkPG was almost instantaneous with maximum effectiveness at pH 3.0. Using the parameters of Langmuir model and a material balance, a filter bed consisting of a stack of GFM was designed for recovering GkPG from a certain volume of culture media. After adsorption and washing, the enzyme was efficiently eluted with a buffer solution at pH 5.0 containing 0.5 M NaCl. The filter bed was reused in several sorption cycles and enzyme recovery could be also done without prior cell removal with the benefits of using microfiltration for enzyme cell separation in a single step. Under the process conditions employed, GkPG recovery was about 80% with a concentration factor of about 4-fold.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82932
Zapata Zapata, Arley D.; Voget, Claudio Enrique; Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters; Elsevier; Process Biochemistry; 47; 8; 8-2012; 1277-1281
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82932
identifier_str_mv Zapata Zapata, Arley D.; Voget, Claudio Enrique; Primary isolation of Geotrichum klebahnii polygalacturonase by capturing with glass fiber microfilters; Elsevier; Process Biochemistry; 47; 8; 8-2012; 1277-1281
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511312001742
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2012.04.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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