Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits

Autores
Rodriguez, Ivana Fabiola; Torres, Sebastián; Sayago, Jorge Esteban; Zampini, Iris Catiana; Isla, Maria Ines; Ordóñez, Roxana Mabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon. The molecular mass of the enzyme was estimated to be 26 kDa by SDS-PAGE. The pH optimum determined was 5.5; the enzyme showed high stability in the pH range between 4.0 and 6.0. The temperature optimum was 37 ºC. Furthermore, a proteinaceous inhibitor of the polygalacturonase (PG) named PGIP was isolated from lemon albedo. This protein was able to maintain the inhibitory activity in a wide range of pH and temperature. The maximal interaction between PG-PGIP was reached after 20 min of contact. PGIP did not show toxic effect on Artemia salina in the concentration range that was active on PG enzyme. Also, the proteinaceous inhibitor did not show mutagenic nor phytotoxic effects.Our results suggest that, the PGIP isolated from lemon could be used as a natural product to decrease the propagation of pathogenic fungi responsible for postharvest diseases in citrus by the inhibition of hydrolytic enzymes secreted by pathogens.
Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Sayago, Jorge Esteban. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
POLYGALACTURONASE
GEOTRICHUM CANDIDUM
PG-INHIBITOR PROTEIN
LEMON FRUITS
PG-PGIP INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43662
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43662
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruitsRodriguez, Ivana FabiolaTorres, SebastiánSayago, Jorge EstebanZampini, Iris CatianaIsla, Maria InesOrdóñez, Roxana MabelPOLYGALACTURONASEGEOTRICHUM CANDIDUMPG-INHIBITOR PROTEINLEMON FRUITSPG-PGIP INTERACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon. The molecular mass of the enzyme was estimated to be 26 kDa by SDS-PAGE. The pH optimum determined was 5.5; the enzyme showed high stability in the pH range between 4.0 and 6.0. The temperature optimum was 37 ºC. Furthermore, a proteinaceous inhibitor of the polygalacturonase (PG) named PGIP was isolated from lemon albedo. This protein was able to maintain the inhibitory activity in a wide range of pH and temperature. The maximal interaction between PG-PGIP was reached after 20 min of contact. PGIP did not show toxic effect on Artemia salina in the concentration range that was active on PG enzyme. Also, the proteinaceous inhibitor did not show mutagenic nor phytotoxic effects.Our results suggest that, the PGIP isolated from lemon could be used as a natural product to decrease the propagation of pathogenic fungi responsible for postharvest diseases in citrus by the inhibition of hydrolytic enzymes secreted by pathogens.Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Sayago, Jorge Esteban. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaSlovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43662Rodriguez, Ivana Fabiola; Torres, Sebastián; Sayago, Jorge Esteban; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 6; 4; 3-2017; 1019-10251338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2017.6.4.1019-1025info:eu-repo/semantics/altIdentifier/url/http://www.jmbfs.org/jmbfs-1105-fabiola/?issue_id=4424&article_id=4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:05Zoai:ri.conicet.gov.ar:11336/43662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:05.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
title Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
spellingShingle Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
Rodriguez, Ivana Fabiola
POLYGALACTURONASE
GEOTRICHUM CANDIDUM
PG-INHIBITOR PROTEIN
LEMON FRUITS
PG-PGIP INTERACTION
title_short Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
title_full Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
title_fullStr Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
title_full_unstemmed Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
title_sort Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits
dc.creator.none.fl_str_mv Rodriguez, Ivana Fabiola
Torres, Sebastián
Sayago, Jorge Esteban
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author Rodriguez, Ivana Fabiola
author_facet Rodriguez, Ivana Fabiola
Torres, Sebastián
Sayago, Jorge Esteban
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author_role author
author2 Torres, Sebastián
Sayago, Jorge Esteban
Zampini, Iris Catiana
Isla, Maria Ines
Ordóñez, Roxana Mabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POLYGALACTURONASE
GEOTRICHUM CANDIDUM
PG-INHIBITOR PROTEIN
LEMON FRUITS
PG-PGIP INTERACTION
topic POLYGALACTURONASE
GEOTRICHUM CANDIDUM
PG-INHIBITOR PROTEIN
LEMON FRUITS
PG-PGIP INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon. The molecular mass of the enzyme was estimated to be 26 kDa by SDS-PAGE. The pH optimum determined was 5.5; the enzyme showed high stability in the pH range between 4.0 and 6.0. The temperature optimum was 37 ºC. Furthermore, a proteinaceous inhibitor of the polygalacturonase (PG) named PGIP was isolated from lemon albedo. This protein was able to maintain the inhibitory activity in a wide range of pH and temperature. The maximal interaction between PG-PGIP was reached after 20 min of contact. PGIP did not show toxic effect on Artemia salina in the concentration range that was active on PG enzyme. Also, the proteinaceous inhibitor did not show mutagenic nor phytotoxic effects.Our results suggest that, the PGIP isolated from lemon could be used as a natural product to decrease the propagation of pathogenic fungi responsible for postharvest diseases in citrus by the inhibition of hydrolytic enzymes secreted by pathogens.
Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Sayago, Jorge Esteban. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon. The molecular mass of the enzyme was estimated to be 26 kDa by SDS-PAGE. The pH optimum determined was 5.5; the enzyme showed high stability in the pH range between 4.0 and 6.0. The temperature optimum was 37 ºC. Furthermore, a proteinaceous inhibitor of the polygalacturonase (PG) named PGIP was isolated from lemon albedo. This protein was able to maintain the inhibitory activity in a wide range of pH and temperature. The maximal interaction between PG-PGIP was reached after 20 min of contact. PGIP did not show toxic effect on Artemia salina in the concentration range that was active on PG enzyme. Also, the proteinaceous inhibitor did not show mutagenic nor phytotoxic effects.Our results suggest that, the PGIP isolated from lemon could be used as a natural product to decrease the propagation of pathogenic fungi responsible for postharvest diseases in citrus by the inhibition of hydrolytic enzymes secreted by pathogens.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43662
Rodriguez, Ivana Fabiola; Torres, Sebastián; Sayago, Jorge Esteban; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 6; 4; 3-2017; 1019-1025
1338-5178
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43662
identifier_str_mv Rodriguez, Ivana Fabiola; Torres, Sebastián; Sayago, Jorge Esteban; Zampini, Iris Catiana; Isla, Maria Ines; et al.; Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits; Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 6; 4; 3-2017; 1019-1025
1338-5178
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.2017.6.4.1019-1025
info:eu-repo/semantics/altIdentifier/url/http://www.jmbfs.org/jmbfs-1105-fabiola/?issue_id=4424&article_id=4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences
publisher.none.fl_str_mv Slovak University of Agriculture in Nitra. Faculty of Biotechnology and Food Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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