Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii

Autores
Cavalitto, Sebastián Fernando; Hours, Roque Alberto; Mignone, Carlos Fernando
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Física
endopolygalacturonases
Geotrichum klebahnii
pectin
pectin-releasing enzymes
protopectinases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/138784
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/138784
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahniiCavalitto, Sebastián FernandoHours, Roque AlbertoMignone, Carlos FernandoCiencias ExactasFísicaendopolygalacturonasesGeotrichum klebahniipectinpectin-releasing enzymesprotopectinasesPectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.Centro de Investigación y Desarrollo en Fermentaciones Industriales1999-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf385-390http://sedici.unlp.edu.ar/handle/10915/138784enginfo:eu-repo/semantics/altIdentifier/issn/0951-208xinfo:eu-repo/semantics/altIdentifier/issn/1573-6784info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1008917912296info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:03:58Zoai:sedici.unlp.edu.ar:10915/138784Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:03:58.479SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
title Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
spellingShingle Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
Cavalitto, Sebastián Fernando
Ciencias Exactas
Física
endopolygalacturonases
Geotrichum klebahnii
pectin
pectin-releasing enzymes
protopectinases
title_short Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
title_full Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
title_fullStr Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
title_full_unstemmed Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
title_sort Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii
dc.creator.none.fl_str_mv Cavalitto, Sebastián Fernando
Hours, Roque Alberto
Mignone, Carlos Fernando
author Cavalitto, Sebastián Fernando
author_facet Cavalitto, Sebastián Fernando
Hours, Roque Alberto
Mignone, Carlos Fernando
author_role author
author2 Hours, Roque Alberto
Mignone, Carlos Fernando
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Física
endopolygalacturonases
Geotrichum klebahnii
pectin
pectin-releasing enzymes
protopectinases
topic Ciencias Exactas
Física
endopolygalacturonases
Geotrichum klebahnii
pectin
pectin-releasing enzymes
protopectinases
dc.description.none.fl_txt_mv Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.
publishDate 1999
dc.date.none.fl_str_mv 1999-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/138784
url http://sedici.unlp.edu.ar/handle/10915/138784
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0951-208x
info:eu-repo/semantics/altIdentifier/issn/1573-6784
info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1008917912296
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
385-390
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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